In order to dissect elite waxy maize germplasm resources from landraces in maize breeding， the genetic diversity 207 Guangxi waxy maize landraces and 6 reference waxy inbred lines was analyzed by genome-wide scanning based on 10K SNP markers. The results showed that the overall genetic diversity of this population was relatively high， with an average expected heterozygosity （He） of 0.31， observed heterozygosity （Ho） of 0.23， and an average polymorphic information content （PIC） of 0.25. The genetic diversity in central Guangxi was the highest （He=0.32， Ho=0.24， MAF=0.24， PIC=0.26） at the population level. These landraces were divided into four major groups by population structure analysis. A correlation on the genetic relationship between populations and the geographical originations of landraces was not observed. In general， the genetic similarity coefficient among Guangxi waxy maize landraces is low， thus suggesting abundant the genetic diversity. Analysis of variance for molecular variation （AMOVA） showed that 99% of the genetic variation is contributed by the intra-population diversity. The genetic differentiation coefficient （F_ST） between groups was 0.008，indicating a low-level differentiation among groups. In summary， the present study unlocked the genetic diversity and relationship of waxy maize landraces in different regions of Guangxi， and provided a theoretical guidance for waxy maize germplasm improvement and breeding of new varieties in Guangxi.