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首页 > 过刊浏览>2023年第24卷第3期 >661-670. DOI:10.13430/j.cnki.jpgr.20221031002
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基于SNP标记揭示广西糯玉米地方品种的遗传多样性与群体遗传结构
作者:
作者单位:

1.广西壮族自治区亚热带作物研究所，南宁 530001;2.广西壮族自治区农业科学院玉米研究所/国家玉米改良中心广西分中心，南宁 530007

作者简介:

研究方向为玉米种质资源研究及遗传育种，E-mail： jingshengfan@yeah.net；

通讯作者:

江禹奉，研究方向为玉米种质资源研究及遗传育种，E-mail： 82668259@qq.com

基金项目:

广西科技厅广西科技基地和人才专项（桂科AD20159093）；广西科技重大专项（桂科AA17204045-2）；广西农业科学院基本科研业务专项 （桂农科2021YT017） ；国家作物种质资源数据中心（NAES018GR03）


Exploring the Genetic Diversity and Population Structure of Guangxi Waxy Maize Landraces Based on SNP Markers
Author:
Affiliation:

1.Guangxi Subtropical Crops Research Institute，Nanning 530001;2.Institute of Maize Research， Guangxi Academy of Agricultural Sciences/Guangxi Sub-center of National Maize Improvement Center， Nanning 530007

Fund Project:

Foundation projects： Guangxi Science and Technology Base and Talent Special Project of Guangxi Science and Technology Department（GuikeAD20159093）； Guangxi Major Science and Technology Projects（GuikeAA17204045-2）； Basic Scientific Research Projects of Guangxi Academy of Agricultural Sciences（Guinongke2021YT017）；National Crop Germplasm Resources Data Center （NAES018GR03）

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    摘要:

    为了挖掘优异糯玉米种质资源，提高糯玉米育种效率，本研究利用10K玉米基因芯片对207个广西糯玉米地方品种和6份参考自交系进行全基因组扫描，解析广西糯玉米地方品种之间的遗传多样性与群体遗传结构。结果表明，广西糯玉米种群总体的遗传多样性较高，平均期望杂合度（He）为0.31，平均观察杂合度（Ho）为0.23，多态信息含量（PIC）平均值为0.25，最小等位基因频率（MAF）平均值为0.23。种群水平上，桂中区域的遗传多样性水平最高（He=0.32，Ho=0.24，MAF=0.24，PIC=0.26）。群体遗传结构分析将地方品种划分为4大类群，种群间遗传关系和类群归属与地理位置不完全相关，品种间总体遗传相似系数偏低，遗传多样性丰富。分子变异方差分析结果显示99%的遗传变异来自种群内。群体间遗传分化系数（FST）为0.008，群体间分化程度较低。研究结果确定了广西不同区域糯玉米地方品种多态性及亲缘关系，为广西糯玉米种质改良和指导新品种培育提供理论依据。

    Abstract:

    In order to dissect elite waxy maize germplasm resources from landraces in maize breeding， the genetic diversity 207 Guangxi waxy maize landraces and 6 reference waxy inbred lines was analyzed by genome-wide scanning based on 10K SNP markers. The results showed that the overall genetic diversity of this population was relatively high， with an average expected heterozygosity （He） of 0.31， observed heterozygosity （Ho） of 0.23， and an average polymorphic information content （PIC） of 0.25. The genetic diversity in central Guangxi was the highest （He=0.32， Ho=0.24， MAF=0.24， PIC=0.26） at the population level. These landraces were divided into four major groups by population structure analysis. A correlation on the genetic relationship between populations and the geographical originations of landraces was not observed. In general， the genetic similarity coefficient among Guangxi waxy maize landraces is low， thus suggesting abundant the genetic diversity. Analysis of variance for molecular variation （AMOVA） showed that 99% of the genetic variation is contributed by the intra-population diversity. The genetic differentiation coefficient （FST） between groups was 0.008，indicating a low-level differentiation among groups. In summary， the present study unlocked the genetic diversity and relationship of waxy maize landraces in different regions of Guangxi， and provided a theoretical guidance for waxy maize germplasm improvement and breeding of new varieties in Guangxi.

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范競升,谢和霞,谢小东,等.基于SNP标记揭示广西糯玉米地方品种的遗传多样性与群体遗传结构[J].植物遗传资源学报,2023,24(3):661-670.

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  • 收稿日期:2022-10-31
  • 最后修改日期:2022-12-14
  • 在线发布日期: 2023-04-27
  • 出版日期: 2023-04-27
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