我国部分审定小麦品种的品质性状及基因型分析
作者:
作者单位:

1.北京市农林科学院杂交小麦研究所/杂交小麦分子遗传北京市重点实验室,北京 100097;2.北京农学院植物科学技术学院,北京 102206

作者简介:

研究方向为杂交小麦遗传育种,E-mail:quanwei360@sina.com

通讯作者:

赵昌平,研究方向为小麦杂种优势利用,E-mail:cp_zhao@vip.sohu.com

中图分类号:

基金项目:

北京市农林科学院科技创新能力建设专项(KJCX20200115, KJCX20200304);国家重点研发计划项目(2016YFD0102000)


Quality Analysis in a Collection of Wheat Varieties Approved in China
Author:
Affiliation:

1.Research Stitute of Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences / Beijing Municipal Key Laboratory of Molecular Genetics of Hybrid Wheat, Beijing 100097;2.College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206

Fund Project:

Foundation projects: Beijing Academy of Agriculture and Forestry Sciences Sci-Tech Innovation Capacity Building Program (KJCX20200115, KJCX20200304); National Key Research and Development Program of China (2016YFD0102000)

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    摘要:

    为了解我国小麦品种的品质相关基因分布及品质性状表现,本研究针对近年来我国审定的530份小麦品种,进行容重、粗蛋白质含量、湿面筋含量、吸水率和稳定时间等性状的分析,同时利用13个品质相关的KASP标记检测基因型,明析不同的品质种植区域内的审定品种优异品质基因等位变异的分布及聚合情况。检测的优异品质基因等位变异在不同区域间的分布不均衡,其中1BL/1RS(-)1Ax 1/1Ax 2*Pinb-D1bPinb-B2b优异等位变异在品质区域间分布频率呈显著性差异,而1Bx17+1By18TaPsy-D1aTaPod-A1b等优异等位变异的分布在区域间无差异;同时进行多个优异等位变异聚合情况分析发现:5个面筋质量相关基因中筛选出同时含有1B/1R(-)1Ax 1/1Ax 2*1Dx5+1Dy10glu-B3g+ 4个优异等位变异的材料12份;3个籽粒硬度相关基因中未检测到同时含有Pina-D1bPinb-D1bPinb-B2b的材料,检测到含有Pina-D1b/Pinb-B2b组合的材料16份,含有Pinb-D1b/Pinb-B2b组合的材料88份。5个籽粒颜色相关基因中同时聚合5个优异等位变异的材料10份。检测的13个品质相关基因KASP标记中,未发现聚合11个以上优异等位变异基因的材料,聚合10个优异等位变异基因的材料有4份,聚合9个优异等位变异的材料有16份。品质分析结果显示:不同区域间品质指标值也存在区域性的差异,且稳定时间与蛋白质含量和湿面筋含量指标不协调。面筋品质相关基因优异等位变异1BL/1RS(-)1Ax1/1Ax2*1Dx5+1Dy10在强筋,中强筋和中筋品种间的分布频率呈极显著差异,且与品质表现呈极显著正相关。

    Abstract:

    In order to clarify the situation of the quality-related genes and corresponding traits of Chinese wheat varieties, 530 wheat varieties released in China in the recent years were analyzed for volume weight, crude protein content, wet gluten content, water absorption and stability time. This collection was genotyped with 13 quality related KASP markers, enabling deciphering the distribution and pyramiding of favorable alleles in wheat-planting areas. The frequency of favorable alleles among different regions was unevenly distributed. The frequencies of 1BL/1RS (-)1Ax 1/1Ax 2* Pinb-D1b and Pinb-B2b were significantly different among different regions, while no difference on the frequency of 1Bx17+1By18TaPsy-D1a and TaPod-A1b among different regions was observed. Twelve genotypes containing four elite alleles of 1B/1R (-)1Ax 1/1Ax 2*1Dx5+1Dy10 and glu-B3g+ at five gluten quality related loci were identified. For three grain hardness genes, the elite alleles combination (Pina-D1b + Pinb-D1b + Pinb-B2b) was not detected, while the combinations of either Pina-D1b + Pinb-B2b or Pinb-D1b + Pinb-B2b were found in 16 and 88 samples, respectively. Ten samples were found containing favorable allelic variants of five color related genes. Four genotypes simultaneously containing 10 favorable alleles were obtained, and 16 varieties with 9 favorable allelic variants were found. The results of quality analysis showed that there were regional differences in quality traits, and the stability time was inconsistent with protein content and wet gluten content. The frequency of elite alleles at gluten quality related genes, such as 1BL/1RS (-)1Ax1/1Ax2* and 1Dx5+1Dy10, was significantly different in the wheat varieties with strong gluten, medium strong gluten and medium gluten, and the allele frequency was positively correlated with the quality.

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引用本文

权威,马锦绣,华正蓉,等.我国部分审定小麦品种的品质性状及基因型分析[J].植物遗传资源学报,2023,24(3):701-718.

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  • 收稿日期:2022-10-31
  • 最后修改日期:2022-12-27
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  • 在线发布日期: 2023-04-27
  • 出版日期: 2023-04-27
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