Agropyron cristatum， as one of the important wild relatives of wheat， contains many excellent genes that are useful in wheat improvement. The wheat-A. cristatum addition line， which contains a complete A. cristatum chromosome， serves as an important bridge to utilize the A. cristatum derived excellent genes. As a perennial tetraploid outcrossing species that carries four haploid sub-genomes， the wheat-A. cristatum additional lines carrying each of different chromosomes from the same homologous group might represent the different agronomic characters. In this study， the heading time， plant type， panicle character， grain character， resistance to powdery mildew and leaf rust were identified and analyzed employing cytological identification， molecular marker detection， agronomic character investigation and disease resistance identification. The wheat-A. cristatum 2P addition line II-9-3 was genetically stable， compact， immune to leaf rust and highly resistant to powdery mildew， which could be used for plant architecture improvement and disease resistance breeding. Wheat-A. cristatum 2P addition line II-3-1b was observed with obvious reduction on plant height， early heading and immune to leaf rust， which could be used for plant height improvement and leaf rust resistance breeding. Wheat-A. cristatum 2P addition line II-23-72 is nearly immune to endemic leaf rust and powdery mildew species， and might be useful in disease-resistance breeding. These three 2P addition lines provided raw materials for further producing 2P translocation and deletion lines. These 2P addition lines with different characteristics on plant type， plant height and disease resistance， might provide a basis for future genetic mapping and effective use of excellent genes applicable in wheat.