1.青岛农业大学园艺学院,青岛 266109;2.山东省园艺作物基因改良工程实验室,青岛 266109
研究方向为番茄遗传育种,E-mail:1097109189@qq.com
朱文莹,研究方向为番茄分子育种与分子生物学,E-mail:zhuwy@qau.edu.cn
山东省农业良种工程项目(2020LZGC005);科技特派员行动计划项目(21-1-4-ny-25-nsh);山东省现代农业产业技术体系项目(SDAIT-05);青岛农业大学博士启动基金(663-1119028)
1.College of Horticulture, Qingdao Agricultural University, Qingdao 266109;2.Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province, Qingdao 266109
Foundation projects: Shandong Province Agricultural Improved Seed Project(2020LZGC005);Project of Action Plan of Science and Technology Commissioner(21-1-4-ny-25-nsh);Shandong Modern Agricultural Industrial Technology System Project(SDAIT-05);Doctor Start-up Fund from Qingdao Agricultural University(663-1119028)
对具有单性结实和自交不亲和特性的番茄自交系85-6进行了研究。通过花的外观观察、花粉活力测定和胚珠发育观察,发现85-6具有正常的花结构,花粉活力正常,胚珠发育正常,表明85-6不存在雌蕊或雄蕊的败育现象。通过自然自交、人工辅助自交和杂交发现,85-6在正常温度环境下存在自交不亲和的现象,自交后得到无籽果实,而作为母本或父本进行杂交时,均可获得杂交后代。在花期遇到温度胁迫(日平均气温<10 ℃或>35 ℃)后,85-6则可完成正常的授粉受精得到正常的有籽果实,但受精后的果实发育迟缓,果实变小,且得到的种子萌发率只有29.75%。在正常温度和温度胁迫条件下,蕾期去雄后均可获得无籽果实,表明85-6番茄具有单性结实的特性。通过对已报道的单性结实基因的序列比对,发现4个单性结实相关结实基因pat-2、pat-k、pad-1、hydra在85-6和79-1之间不存在基因序列上的差异,推测控制85-6单性结实的基因可能是一个新的基因。85-6番茄材料的发现丰富了番茄单性结实和自交不亲和资源,为番茄的单性结实和自交不亲和育种以及相关机制的研究提供了较好的重要材料。
This study investigated a special tomato inbred line 85-6 with the parthenocarpy and self-incompatibility. Through the observation of flower appearance, pollen viability and ovule development, 85-6 was found with normal flower structure, pollen activity and ovule development, thus ruling out the abortion of pistil or stamen. Based on the fertility variations of self-pollination, and artificially-assisted self-pollination and hybridization experiments at different temperature, 85-6 showed self-incompatibility under normal temperature conditions, and produced seedless fruit after self-pollination, as well as normal hybrid offspring whenever serving as male or female parents. Under temperature stress (daily average temperature <10 ℃ or >35 ℃) at flowering stage, 85-6 became complete pollination and fertilization to obtain seeded fruits, and its offspring seeds showed 29.75% seed germination rate. After emasculation at the bud stage, whenever under normal or lower temperature conditions, t85-6 was able to produce seedless fruit, thus indicating the characteristics of parthenocarpy. Collectively, the material of 85-6, as a newly-identified germplasm showing the parthenocarpy and self-incompatibility, provided a basis for the parthenocarpy and self-incompatibility breeding, and further deciphering the functional mechanisms.
曹依林,曹作增,韩欣静,等.番茄自交系