2025年4月4日 3:10 星期五
首页 > 过刊浏览>2023年第24卷第5期 >1367-1379. DOI:10.13430/j.cnki.jpgr.20230311001
PDF HTML阅读 XML下载 导出引用 引用提醒
榕江茶（Camellia yungkiangensis）种质资源主要品质性状的遗传多样性分析
作者:
作者单位:

1.贵州大学茶学院，贵阳 550025;2.榕江县农业农村局，贵州榕江 557200;3.榕江县两汪乡人民政府，贵州榕江 557205

作者简介:

研究方向为茶树遗传育种与资源高效利用，E-mail：3122274539@qq.com

通讯作者:

陈涛林，主要从事茶树遗传育种与资源高效利用研究工作，E-mail：chentaolinteac08@163.com

基金项目:

国家自然科学基金项目（32260787）；贵州省科技支撑计划项目（黔科合支撑［2021］一般188）；贵州大学人才引进项目（贵大人基合字［2019］25号）


Genetic Diversity Analysis of Main Quality Traits in Camellia yungkianensis Germplasm Resources
Author:
Affiliation:

1.Tea College of Guizhou University， Guiyang 550025;2.Rongjiang County Bureau of Agriculture and Rural Affairs， Rongjiang 557200， Guizhou;3.Liangwang Township People’s Government of Rongjiang County， Rongjiang 557205， Guizhou

Fund Project:

Foundation projects： National Natural Science Foundation of China Project（32260787）； Science and Technology Project of Guizhou Province（Qian Ke He Support ［2021］ General 188）； Talent Introduction Project of Guizhou University （Gui Da Ren Ji He Zi ［2019］ No. 25）

  • 摘要
    • | |
  • 访问统计
    • |
  • 参考文献 [40]
    • | | | |
  • 文章评论
    摘要:

    本研究分析了贵州特异茶树种质资源——榕江茶的品质化学成分特征及其遗传多样性，为其开发利用和特异茶树新品种培育提供科学依据。以分布在贵州省黔东南州月亮山的121份榕江茶种质资源为材料，对其水浸出物、茶多酚、游离氨基酸等21个主要品质化学成分指标进行检测分析，通过遗传多样性分析、主成分分析、聚类分析、相关性分析等对其品质性状特征和遗传多样性进行评价，并对其中的优异资源进行筛选。121份资源的21个品质化学成分指标的变异系数为5.70%~119.69%，平均为32.84%，遗传多样性指数为1.47~2.08，平均为1.95。21个品质化学成分指标的相关性分析结果显示，呈极显著正相关有42对性状，呈显著正相关的有14对，呈极显著负相关的有10对，呈显著负相关的有10对。主成分分析表明，前7个主成分特征值均大于1，且累计贡献率达76.84%，包含了原始变量的绝大部分信息，以因子得分系数矩阵求得每个单株资源的品质化学成分在各主成分上的综合得分，筛选出了12份品质化学成分综合得分高的单株资源。聚类分析显示，在欧式距离18.0处，可将121份榕江茶种质资源分为6个类群。优异种质资源筛选结果显示，121份榕江茶种质资源均属于高茶多酚（≥20.0%）、低咖啡碱（≤1.5%）、高可可碱（≥2.5%）类型的资源，其中高茶多酚（≥20.0%）、高水浸出物（≥45%）的特异类型资源有72份。榕江茶种质资源是高茶多酚、低咖啡碱、高可可碱类型的资源，其品质化学成分具有丰富的遗传多样性，具有培育特异茶树新品种的潜力。

    Abstract:

    This study analyzed the quality chemical composition characteristics and genetic diversity of Camellia yungkiangensis， a special tea germplasm resource in Guizhou， in order to provide scientific basis for its development and utilization and the cultivation of new special tea varieties. 121 Camellia yungkiangensis germplasms collected from Moon Mountain， Qiandongnan Prefecture， Guizhou Province， China， were subjected to analyze 21 main quality chemical components， such as water extracts， tea polyphenols， free amino acids. Their quality characteristics and genetic diversity were evaluated by genetic diversity analysis， principal component analysis， cluster analysis， correlation analysis， and the elite resources were identified. The variation coefficient of 21 quality chemical components in 121 resources was 5.70-119.69， with an average of 32.84%， and the genetic diversity index was 1.47-2.08， with an average of 1.95. Based on the correlation analysis results of 21 quality chemical component indexes， there were 42 pairs of traits with extremely significant positive correlation， 14 pairs with significant positive correlation， 10 pairs with extremely significant negative correlation， and 10 pairs with significant negative correlation. The principal component analysis showed that the characteristic values of the first seven principal components were over 1， with the cumulative contribution rate of 76.84%. The comprehensive score of the quality chemical components of each individual plant resource on each principal component was obtained by the factor score coefficient matrix， and 12 individual plant resources with high comprehensive score of the quality chemical components were selected. Cluster analysis showed that 121 Camellia yungkiangensis germplasm resources could be divided into 6 groups at the Euclidean distance of 18.0. The 121 Camellia yungkiangensis germplasm resources were identified with high tea polyphenols （≥20.0%）， low caffeine （≤1.5%） and high theobromine （≥2.5%）， of which 72 were high tea polyphenols （≥20.0%） and high water extracts （≥45%）. These Camellia yungkiangensis germplasm resources， which showed high tea polyphenols， low caffeine and high theobromine， represent rich genetic diversity and have the potential to cultivate new varieties of specific tea plants.

    参考文献
    [1] 陈亮,杨亚军,虞富莲. 中国茶树种质资源研究的主要进展和展望. 植物遗传资源学报, 2004,5(4): 389-392Chen L, Yang Y J, Yu F L. Tea germplasm research in China:Recent progresses and prospects. Journal of Plant Genetic Resources, 2004,5(4):389-392
    [2] 陈涛林. 广西元宝山一种特异茶饮植物的系统学鉴定与综合评价研究. 长沙:湖南农业大学, 2019Chen T L. Systematic identification and comprehensive evaluation research on a specific tea plant of Yuanbao mountain in Guangxi. Changsha:Hunan Agricultural University,2019
    [3] 姜燕华. 我国茶树地方品种遗传多样性及人为选择影响的研究. 北京:中国农业科学院, 2010Jiang Y H. Studies on the genetic diversity of traditional tea germplasm in China as well as the influence of artificial selection. Beijing:Chinese Academy of Agricultural Sciences, 2010
    [4] Kottawa-Arachchi J D, Gunasekare M T K, Ranatunga M A B. Biochemical diversity of global tea [Camellia sinensis (L.) O. Kuntze] germplasm and its exploitation: A review. Genetic Resources and Crop Evolution, 2019(66), 259-273
    [5] 杨亚军. 茶树育种品质早期化学鉴定—Ⅱ.鲜叶的主要生化组分与绿茶品质的关系. 茶叶科学,1991(2):127-131Yang Y J. Chemical evaluation on tea quality durmg early-stage of breeding program Ⅱ. Relationship between the biochemical component content in the shoots and the quality of green tea. Journal of Tea Science, 1991(2):127-131
    [6] 陆锦时,魏芳华,李春华. 茶树品种主要化学成份与品质关系的研究. 西南农业学报,1994(S1):1-5Lu J S ,Wei F H, Li C H. A study on the relationship between main chemical components and quality of tea varieties. Southwest China Journal of Agricultural Sciences, 1994 (S1):1-5
    [7] 牛素贞,宋勤飞,安红卫,黄政,刘霞,赵德刚,陈正武. 贵州古茶树种质资源基于形态特征的多样性研究. 浙江农业学报,2019,31(10):1689-1699Niu S Z, Song Q F, An H W, Huang Z, Liu X, Zhao D G, Chen Z W. Diversity of ancient tea germplasm based on morphological characteristics in Guizhou. Acta Agriculturae Zhejiangensis, 2019, 31(10):1689-1699
    [8] 陈正武,陈娟,龚雪,张小琴. 28份贵州茶树种质资源的生化成分多样性分析. 西南农业学报,2015,28(4):1517-1523Chen Z W, Chen J, Gong X, Zhang X Q. Diversity analysis for biochemical components of 28 tea germplasm resources in Guizhou. Southwest China Journal of Agricultural Sciences, 2015, 28(4):1517-1523
    [9] 刘声传,段学艺,赵华富,魏杰,郭燕,鄢东海. 贵州野生茶树种质资源生化多样性分析. 植物遗传资源学报,2014,15(6):1255-1261Liu S C, Duan X Y, Zhao H F, Wei J, Guo Y, Yan D H. Biochemical diversity analysis of wild tea germplasms in Guizhou. Journal of Plant Genetic Resources, 2014, 15(6):1255-1261
    [10] 段学艺,王家伦,胡华健,高秀兵. 贵州西南部大树茶生化特性研究. 中国农学通报,2012,28(19):286-289Duan X Y, Wang J L, Hu H J, Gao X B. Study on the biochemical components of tea Camellias in southwest Guizhou province. Chinese Agricultural Science Bulletin, 2012, 28(19):286-289
    [11] 鄢东海,刘声传,罗显扬,魏杰,陆建良,范方媛. 贵州地方茶树品种资源遗传多样性RAPD分析. 中国农学通报,2015,31(19):30-34Yan D H, Liu S C, Luo X Y, Wei J, Lu J L, Fan F Y. Analysis of genetic diversity with RAPD markers for local tea populations in Guizhou. Chinese Agricultural Science Bulletin, 2015, 31(19):30-34
    [12] 安红卫,宋勤飞,牛素贞. 贵州茶树种质资源遗传多样性、群体结构和遗传分化研究. 浙江农业学报,2021,33(7):1234-1243An H W, Song Q F, Niu S Z. Study on genetic diversity, population structure and genetic differentiation of tea germplasm in Guizhou. Acta Agriculturae Zhejiangensis, 2021, 33(7):1234-1243
    [13] 张明泽,姚玉仙,陈世军. 黔南60份茶树种质资源遗传多样性的SSR分析. 西北植物学报,2016,36(6):1117-1124Zhang M Z, Yao Y X, Chen S J. Genetic diversity analysis of tea germplasm in qiannan prefecture by SSR markers. Acta Botanica Boreali-Occidentalia Sinica, 2016, 36(6):1117-1124
    [14] 郭燕,刘声传,曹雨,赵华富,魏杰,鄢东海,周富裕. 基于SSR标记贵州古茶树资源的遗传多样性分析及指纹图谱构建. 西南农业学报,2016,29(3):491-497Guo Y, Liu S C, Cao Y, Zhao H F, Wei J, Yan D H, Zhou F Y. Analysis of genetic diversity and construction of molecular fingerprinting with SSR Markers for ancient tea germplasms in Guizhou. Southwest China Journal of Agricultural Sciences, 2016, 29(3):491-497
    [15] 张正竹. 茶叶生物化学实验教程. 北京:中国农业出版社,2009: 44-45Zhang Z Z. Experimental course of tea biochemistry. Beijing:China Agricultural Press,2019: 44-45
    [16] 马陶陶,张群林,李俊,孟晓明,黄成,陈玉璞. 三氯化铝比色法测定中药总黄酮方法的探讨. 时珍国医国药,2008(1):54-56Ma T T, Zhang Q L, Li J, Meng X M, Huang C, Chen Y P. AlCl3 Colorimetry for determination of total flavonoids. Lishizhen Medicine and Materia Medica, 2008(1):54-56
    [17] 王丽丽,陈键,宋振硕,陈林.茶叶中没食子酸、儿茶素类和生物碱的HPLC检测方法研究.福建农业学报,2014,29(10):987-994Wang L L, Chen J, Song Z S, Chen L. Simultaneous HPLC determination of gallic acid, catechins and alkaloids in tea. Fujian Journal of Agricultural Sciences,2014,29(10):987-994
    [18] 杨金川,白雪梅. HPLC法同时测定茶叶中儿茶素类和咖啡因的含量. 贵州农业科学,2020,48(2):99-102Yang J C, Bai X M. Simultaneous determination of catechins and caffeine content in tea by HPLC. Guizhou Agricultural Sciences, 2020,48(2):99-102
    [19] 周波,江海东,张秀新,薛璟祺,石颜通. 部分引进牡丹品种的形态多样性. 生物多样性,2011,19(5):543-550Zhou B, Jiang H D, Zhang X X, Xue J Q, Shi Y T. Morphological diversity of some introduced tree peony cultivars. Biodiversity Science, 2011, 19(5):543-550
    [20] 陈亮,虞富莲,杨亚军.茶树种质资源与遗传改良. 中国茶叶,2007(2):11Chen L, Yu F L, Yang Y J. Tea germplasm resources and genetic improvement. China Tea, 2007(2):11
    [21] 鄢东海,罗显扬,魏杰,陈元安,刘红梅. 贵州地方茶树资源的生化成分多样性及绿茶品质. 中国农学通报,2010,26(3):81-85Yan D H, Luo X Y, Wei J, Chen Y A, Liu H M. Blochemical compopents diversity and the quality of green tea on local tea germplasm resources in Guizhou. Chinese Agricultural Science Bulletin, 2010, 26(3):81-85
    [22] 李小胜,陈珍珍. 如何正确应用SPSS软件做主成分分析. 统计研究,2010,27(8):105-108Li X S, Chen Z Z. Correctly using SPSS software for principal components analysis. Statistical Research, 2010, 27(8):105-108
    [23] 喻华平,赵志常,高爱平,罗睿雄. 基于主成分分析和聚类分析的23份黄皮种质资源的品质评价. 热带作物学报,2022,43(7):1357-1364Yu H P, Zhao Z C, Gao A P, Luo R X. Quality evaluation of 23 species of Clausena lansium (Lour. ) skeels germplasm resources based on principal component analysis and cluster analysis. Chinese Journal of Tropical Crops, 2022, 43(7):1357-1364
    [24] 李庆伟. 日照绿茶儿茶素品质指数研究. 农学学报,2014,4(6):64-66Li Q W. Rizhao green tea catechin quality index. Journal of Agronomy, 2014, 4(6):64-66
    [25] 赵华富,高秀兵,刘晓霞,曹雨,周顺珍,周国兰. 贵州高茶多酚茶树品种多酚品质分析评价. 中国农学通报,2016,32(16):149-154Zhao H F, Gao X B, Liu X X, Cao Y, Zhou S Z, Zhou G L. Analysis and evaluation of polyphenols quality of high polyphenols content tea varieties in Guizhou. Chinese Agricultural Science Bulletin, 2016,32(16):149-154
    [26] 马圣洲,赵飞,胡莹,袁兴兴,杨亦扬. 江苏丘陵地区主栽茶树品种的红茶适制性研究. 中国农学通报,2022,38(26):32-38Ma S Z, Zhao F, Hu Y, Yuan X X, Yang Y Y. Tea cultivars from the hilly areas of Jiangsu province: Suitability for black tea processing. Chinese Agricultural Science Bulletin, 2022, 38(26):32-38
    [27] 陈亮,姚明哲,王新超,马春雷,金基强,杨亚军,江用文,熊兴平,王平盛 . NY/T 2031—2011 农作物优异种质资源评价规范 茶树 . 北京:中华人民共和国农业农村部,2011Chen L,Yao M Z,Wang X C,Ma C L,Jin J Q,Yang Y J,Jiang Y W,Xiong X P,Wang P S. NY/T 2031—2011 evaluation specifications of excellent crop germplasm resources for tea plant. Beijing:Ministry of Agriculture of the People’s Republic of China,2011
    [28] 葛菁,庞磊,李叶云,江昌俊. 茶树可溶性糖含量的HPLC-ELSD检测及其与茶树抗寒性的相关分析. 安徽农业大学学报,2013,40(3):470-473Ge J, Pang L, Li Y Y, Jiang C J. Determination of soluble sugar in tea plant by HPLC-ELSD and its relationship with freezing resistance. Journal of Anhui Agricultural University, 2013, 40(3):470-473
    [29] 朱政,蒋家月,江昌俊,李雯. 低温胁迫对茶树叶片SOD、可溶性蛋白和可溶性糖含量的影响. 安徽农业大学学报,2011,38(1):24-26Zhu Z, Jiang J Y, Jiang C J, Li W. Effects of low temperature stress on SOD activity, soluble protein content and soluble sugar content in Camellia sinensis leaves. Journal of Anhui Agricultural University, 2011,38(1):24-26
    [30] 李叶云,舒锡婷,周月琴,江昌俊. 自然越冬过程中3个茶树品种的生理特性变化及抗寒性评价. 植物资源与环境学报,2014,23(3):52-58Li Y Y, Shu X T, Zhou Y Q , Jiang C J. Change in physiological characteristics and cold resistance evaluation of three cultivars of Camellia sinensis during natural overwintering period. Journal of Plant Resources and Environment, 2014, 23(3):52-58
    [31] 亓白岩,周冬琴,於朝广,徐和宝,殷云龙. 8种含笑属植物的抗寒性研究. 江苏农业科学,2010(5):258-263Qi B Y ,Zhou D Q ,Yu C G ,Xu H B ,Yin Y L. Study on cold resistance of 8 species of Michelia. Jiangsu Agricultural Sciences, 2010 (5):258-263
    [32] 宁静,刘振,杨拥军,杨阳,沈程文,张曙光,肖敦旺,马良元. 城步峒茶资源主要生化成分遗传多样性分析. 茶叶通讯,2019,46(3):269-275Ning J, Liu Z, Yang Y J, Yang Y, Shen C W, Zhang S G, Xiao D W, Ma L Y. Genetic diversity analysis for main biochemical components of Chengbu Dong tea germplasm resources. Tea Newsletter, 2019, 46(3):269-275
    [33] 王新超,陈亮,杨亚军. 广西茶树资源生化成分多样性分析. 植物遗传资源学报,2010,11(3):309-314,319Wang X C, Chen L, Yang Y J. Biochemical diversity analysis of tea germplasms in Guangxi. Journal of Plant Genetic Resources, 2010, 11(3):309-314,319
    [34] 蒋会兵,田易萍,陈林波,梁名志. 云南茶树地方品种农艺性状与品质性状遗传多样性分析. 植物遗传资源学报,2013,14(4):634-640Jiang H B, Tian Y P, Chen L B, Liang M Z. Diversity of tea landraces based on agronomic and quality traits in Yunnan province. Journal of Plant Genetic Resources , 2013, 14(4):634-640
    [35] 张泽岑. 对茶树早期鉴定品质指标和酚氨比的一点看法. 茶叶通讯,1991(3):22-25Zhang Z C. Some opinions on the early identification quality index and phenol-ammonia ratio of tea plants. Journal of Tea Communication, 1991(3):22-25
    [36] 周卫龙,许凌,徐建峰,沙海涛,陆小磊. GB/T8313-2008 第二法 茶叶中茶多酚测定的研究比较. 中国茶叶加工,2009(1):40-41Zhou W L, Xu L, Xu J F, Sha H T, Lu X L. Study and comparison of the determination of tea polyphenols in tea by the second method(GB/T8313-2008). China Tea Processing, 2009(1):40-41
    [37] 周顺珍,周国兰,何萍,郑文丽. 不同测定方法对绿茶中茶多酚含量测定结果的影响. 化学分析计量,2011,20(1):88-89Zhou S Z, Zhou G L, He P, Zheng W L. Effect of different determination methods on the determination results of tea polyphenols in green tea. Chemical Analysis and Meterage, 2011,20(1):88-89
    [38] 翟秀明,李解,唐敏,胡方洁,张军,侯渝嘉,徐泽. 重庆30份茶树种质资源农艺性状与生化成分多样性. 浙江农业学报,2021,33(7):1244-1255Zhai X M, Li J, Tang M, Hu F J, Zhang J, Hou Y J, Xu Z. Diversity analysis of 30 tea Camelia sinensis germplasm resources in Chongqing based on agronomic traits and biochemical components. Acta Agriculturae Zhejiangensis,2021,33(7):1244-1255
    [39] 黄飞毅,陈宇宏,刘伟,丁玎,雷雨,段继华,邓晶,康彦凯,罗意,张秀军,刘硕谦,李赛君.湖南莽山茶树种质资源调查与品质性状的遗传多样性分析.植物遗传资源学报,2021,22(2):328-337Huang F Y, Chen Y H, Liu W, Ding D, Lei Y, Duan J H, Deng J, Kang Y K, Luo Y, Zhang X J, Liu S Q, Li S J. Germplasm resources and genetic diversity of quality characters of tea plants from Mangshan in Hunan. Journal of Plant Genetic Resources, 2021,22(2): 328-337
    [40] 班秋艳,潘宇婷,胡歆,纪晓明,余有本,任华江,丁帅涛,闫满朝,江昌俊.陕西茶树地方种质资源特征性生化成分分析.安徽农业大学学报, 2018,45(5):777-782Ban Q Y, Pan Y T, Hu X, Ji X M, Yu Y B, Ren H J, Ding S T, Yan M C, Jiang C J. Characteristic biochemical analysis of tea germplasm resources in Shaanxi province. Journal of Anhui Agricultural University, 2018, 45(5):777-782
    相似文献
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

吴河饶,任青艳,陈莹,等.榕江茶（Camellia yungkiangensis）种质资源主要品质性状的遗传多样性分析[J].植物遗传资源学报,2023,24(5):1367-1379.

复制
分享
文章指标
  • 点击次数:224
  • 下载次数: 882
  • HTML阅读次数: 90
  • 引用次数: 0
历史
  • 收稿日期:2023-03-11
  • 最后修改日期:2023-04-05
  • 在线发布日期: 2023-08-30
  • 出版日期: 2023-08-30
文章二维码
您是第5741277位访问者
ICP:京ICP备09069690号-23
京ICP备09069690号-23
植物遗传资源学报 ® 2025 版权所有
技术支持：北京勤云科技发展有限公司
请使用 Firefox、Chrome、IE10、IE11、360极速模式、搜狗极速模式、QQ极速模式等浏览器，其他浏览器不建议使用!