江苏省农业科学院粮食作物研究所,南京210014
研究方向为小麦遗传与育种,E-mail : mpzhou2000@163.com
江苏省农业科技自主创新资金项目(CX(19)1001);江苏省重点研发计划(BE2021375);国家小麦产业体系(CARS-03-57)
Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014
Jiangsu Agricultural Science and Technology Innovation Fund (CX(19)1001);The Key Research and Development Program of Jiangsu Province(BE2021375);China Agriculture Research System(CARS-03-57)
小麦籽粒硬度是影响小麦商品分类分级、小麦制粉工艺和小麦面粉最终加工用途的重要指标,分子标记辅助选择可以有效提高小麦籽粒硬度的选育效率。为了发掘和开发更多与小麦籽粒硬度紧密连锁的分子标记,本研究采用硬质小麦扬麦158与软质小麦西风配制重组自交系群体,利用55K SNP芯片分析群体基因型并构建了群体遗传连锁图,结合4年群体籽粒硬度的表型资料对影响小麦籽粒硬度的QTL进行了分子定位。结果显示,构建的遗传连锁图覆盖2784.9 cM,含有3830个非共分离的SNP标记;除PIN基因外,共定位到12个可重复的QTL位点,分别位于1A、1B、1D、2A(2个)、3A、4D、5A、5D、6B、6D和7A染色体,单个QTL可解释3.2%~15.2%籽粒硬度变异;11个QTL来自软质小麦西风,1个QTL由硬质小麦扬麦158贡献;7个QTL表现稳定,可在4年试验中重复,其中5个QTL未见报道,为新发现QTL,特别是5D染色体新发现的QTL最高可解释15.2%表型变异。与这些稳定QTL紧密连锁的SNP标记将为今后开展长江中下游麦区软质小麦的分子标记辅助选择提供帮助。
The wheat grain hardness is an important index affecting the commodity classification, milling technology and flour final processing use. Molecular marker-assisted (MAS) technique can effectively improve the breeding efficiency for grain hardness in wheat. In order to explore and develop more molecular markers that are closely linked to the grain hardness of wheat, the recombinant inbred lines (RILs) population was constructed by crossing the hard wheat Yangmai 158 with soft wheat Xifeng, and genotyped by wheat 55K SNP array technique. The genetic linkage map is 2784.9 cM in length with 3830 non-consegregation SNP markers. Based on the kernel hardness phenotype in the 4-year experiment, the QTL affecting wheat grain hardness were mapped in the chromosome. A total of 12 repeatable QTL were identified on chromosomes 1A, 1B, 1D, 2A (2), 3A, 4D, 5A, 5D, 6B, 6D and 7A, and each single QTL could explain 3.2%-15.2% of grain hardness variation. Among them, eleven QTL were derived from the soft wheat Xifeng, and one QTL from the hard wheat Yangmai 158. Seven QTL showed stable performance and were detected simultaneously in the 4-year experiment, and five QTL were newly identified. The newly discovered QTL, especially QTL on chromosome 5D, could account for up to 15.2% of the phenotypic variation. SNP markers closely linked to these QTL will provide help for marker-assisted selection in soft wheat in the middle and lower reaches of the Yangtze River.
周淼平,杨学明,张鹏,等.基于重组自交系群体的小麦籽粒硬度