河北农业大学林学院/河北省林木种质资源与森林保护重点实验室,保定071000
研究方向为林木遗传育种,E-mail :2429132584@qq.com
王进茂,研究方向为林木遗传育种,E-mail :lxwjm@hebau.edu.cn
蒙古栎种质资源收集保存及遗传评价(KJZXSA202207)
College of Forestry Science, Agricultural University of Hebei/Hebei Key Laboratory of Tree Genetic Resources and Forest Protection, Baoding 071000
Collection, Conservation and Genetic Evaluation of Quercus mongolica Germplasm Resources(KJZXSA202207)
蒙古栎(Quercus mongolica Fisch.)原产于东亚地区的温带,是珍贵的用材树种,具备很高的应用价值和经济价值。Trihelix转录因子主要与植物光响应、生长发育和响应非生物胁迫等方面相关。为了研究蒙古栎Trihelix转录因子在不同遮阴处理和水分胁迫下的表现,本研究运用生物信息学分析,从蒙古栎基因组中鉴定出34个Trihelix转录因子基因,依次命名为:QmTH01~QmTH34。与拟南芥中鉴定到的29个Trihelix转录因子进行聚类分析,可将其分为GT-1、GT-2、GTγ、SH4、SIP1五个亚族,34个QmTHs分别定位到蒙古栎的10条染色体上,翻译蛋白范围为189~897个氨基酸,等电点为4.58~9.78。QmTHs启动子区域共鉴定出14种不同的与光响应、非生物胁迫、激素以及生长发育响应调控相关的顺式作用元件,其中茉莉酸甲酯响应元件、脱落酸响应元件和光响应元件数量占据主要地位。根据不同遮阴处理和水分胁迫下基因表达分析,鉴定到QmTH01、QmTH14、QmTH22、QmTH24、QmTH33在高光强下表达量相对较高,并随光照强度减弱显著下调表达,表明这5个基因参与蒙古栎响应高光照下的生长生理。鉴定到QmTH06、QmTH17、QmTH24在5次(4、5、6、7、8月)浇水的水分胁迫处理中显著上调表达,表明这3个基因介导蒙古栎响应水分胁迫。
Quercus mongolica Fisch is a native species at the temperate zone of East Asia, and is highly valuable considering its application and economic potential. Trihelix transcription factors are related to plant light response, growth and development, and abiotic stress. In order to study the performance of Trihelix transcription factors in Quercus mongolica under different shading and water stress, 34 QmTHs (designated QmTH01 to QmTH34) were identified from the genome of Quercus mongolica by bioinformatics analysis. By clustering analysis with 29 Trihelix transcription factors identified in Arabidopsis thaliana, the Trihelix transcription factors can be divided into five subgroups including GT-1, GT-2, GT-γ, SH4 and SIP1. QmTHs were found on 10 chromosomes of Quercus mongolica, encoding for the putative proteins ranged from 189 to 897 aa, with isoelectric points ranging from 4.58 to 9.78. A total of 14 different cis-acting elements were identified in the promoters of QmTHs, in which the elements related to methyl jasmonate response, abscisic acid response and light response were often found. According to gene expression analysis under different shading and water stress, the transcripts of QmTH01, QmTH14, QmTH22, QmTH24 and QmTH33 were relatively high under high light intensity, and significantly down-regulated with the decrease of light intensity, indicating that these five genes were involved in the growth physiology of Quercus mongolica under high light response. The expressions of QmTH06, QmTH17 and QmTH24 were significantly up-regulated under watering treatments (April, May, June, July and August; once per month), indicating that these genes possibly mediated the response of Quercus mongolica to water stress.
孟鑫,王世杰,江敏,等.蒙古栎