1.安徽农业大学农学院, 合肥 230036;2.中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/农业农村部北京大豆生物学重点实验室, 北京 100081;3.东北林业大学生命科学学院,黑龙江哈尔滨 150040;4.东北农业大学农学院,黑龙江哈尔滨 150030
研究方向为大豆优异基因挖掘,E-mail: 985874731@qq.com
邱丽娟,研究方向为大豆基因资源挖掘与利用,E-mail: qiulijuan@caas.cn
中国农业科学院创新工程基金项目
ZHOU Ya
School of Agronomy,Anhui Agricultural University,Hefei 230036;Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Soybean Biology in Beijing,Ministry of Ariculture and Rural Affairs, Beijing 100081ZHANG Xiang
School of Agronomy,Anhui Agricultural University,Hefei 230036;Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Soybean Biology in Beijing,Ministry of Ariculture and Rural Affairs, Beijing 100081ZHAO Quan
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Soybean Biology in Beijing,Ministry of Ariculture and Rural Affairs, Beijing 100081;College of Life Sciences, Northeast Forestry University, Harbin 150040, HeilongjiangSUN Jianqiang
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Soybean Biology in Beijing,Ministry of Ariculture and Rural Affairs, Beijing 100081;College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang1.School of Agronomy,Anhui Agricultural University,Hefei 230036;2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Soybean Biology in Beijing,Ministry of Ariculture and Rural Affairs, Beijing 100081;3.College of Life Sciences, Northeast Forestry University, Harbin 150040, Heilongjiang;4.College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang
Foundation project: The Innovation Project of Chinese Academy of Agricultural Sciences
双单倍体技术被广泛应用于加速植物育种,近年来,利用含有功能未知结构域DUF679膜蛋白(DMP)突变的玉米(Zea mays L.)株系作为单倍体诱导株系。本研究搜索与玉米DMP基因氨基酸序列同源率60%以上的大豆DMP基因,对其进行生物信息学分析;并结合2214份重测序数据库探究GmDMP基因在大豆中的分子机理及生物多样性。分析结果表明: GmDMP1(Glyma.18G097400)与GmDMP2(Glyma.18G098300),与玉米DMP基因的进化关系亲缘度极高,基因全长均为645 bp,氨基酸序列同源率高达95%以上,编码氨基酸数目与等电点完全一致,磷酸化分布仅有1个位点差异。GmDMP1与GmDMP2基因均有1个相同的结构域DUF679,定位于内质网的可能性较大,均为跨膜的不分泌亲水蛋白。GmDMP1与GmDMP2基因在2214份种质资源中分别发生了3个与1个非同义突变,各组成3种和2种单倍型。GmDMP1H3在驯化中受到了强烈的选择,GmDMP1H1与GmDMP1H2单倍型的突变位点在结构域DUF679上。将大豆的两个DMP基因突变后可能获得单倍体诱导系,从而缩短大豆育种年限。
Double haploid techniques have been widely used to accelerate plant breeding, and in recent years, maize (Zea mays L.) lines containing mutations in the membrane protein (DMP) of the functionally unknown domain DUF679 have been used as haploidy-induced lines. In this study, soybean DMP genes with more than 60% homology with maize DMP gene were searched and bioinformatic analysis was performed. The molecular mechanism and biodiversity of GmDMP gene in soybean were investigated by using 2214 resequencing databases. The analysis results show that: GmDMP1 (Glyma.18G097400) and GmDMP2 (Glyma.18G098300) have high evolutionary affinity with maize DMP gene. The full length of genes is 645 bp, the homology rate of amino acid sequence is over 95%, and the number of encoded amino acids is completely consistent with the isoelectric point. The phosphorylation distribution differs at only one site. Both GmDMP1 and GmDMP2 genes have the same domain DUF679, which is more likely to locate in the ER, and both are transmembrane non-secreting hydrophilic proteins. There were 3 and 1 non-synonymous mutations of GmDMP1 and GmDMP2 genes in 2214 germplasm resources, respectively, consisting of 3 and 2 haplotypes. GmDMP1H3 has been strongly selected during domestication, and the mutation site of the GmDMP1H1 and GmDMP1H2 haplotypes is located in the DUF679 domain. It is possible to obtain a haploid inducible line by mutating two DMP genes in soybean, thus shortening the breeding life of soybean.
周雅,张祥,赵权,等.大豆