小麦主胚根生长及主要性状全基因组关联分析
作者:
作者单位:

河南农业大学农学院/国家小麦工程技术研究中心/省部共建小麦玉米作物学国家重点实验室/河南粮食作物协同创新中心,郑州 450046

作者简介:

研究方向为小麦生理生态,E-mail:nxy_yangjian@stu.henau.edu.cn

通讯作者:

杨习文,研究方向为小麦生理生态,E-mail:yangxiwen@henau.edu.cn
贺德先,研究方向为小麦生理生态,E-mail:hed@henau.edu.cn

中图分类号:

基金项目:

国家重点研发计划(2022YFD2300802)


Genome-wide Association Analysis of Radicle Growth and Morphological Traits in Wheat (Triticum aestivum L.)
Author:
Affiliation:

College of Agronomy , Henan Agricultural University/National Engineering Research Center for Wheat/Co-Construction State Key Laboratory of Wheat and Maize Crop Science/Collaborative Innovation Center of Henan Grain Crops, Zhengzhou 450046

Fund Project:

Foundation project: National Key Research and Development Program (2022YFD2300802)

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    摘要:

    为解析小麦初生根系建成的遗传机制,本研究以黄淮麦区的198份小麦自然群体为材料,对在室内人工气候箱内水培21 d的小麦主胚根的一级分枝根数、分枝密度、长度、表面积、体积和平均直径6个性状进行调查分析,结合660K基因芯片用Q+K混合线性模型对主胚根性状进行全基因组关联分析,并对显著且稳定的关联位点进行功能注释和候选基因挖掘。结果表明,主胚根不同性状呈正态或近似正态分布,变异系数为5.56%~22.10%。通过全基因组关联分析,共检测到136个显著关联位点,这些位点分布在除7B以外的染色体上,可解释5.10%~13.60%的表型变异,同时检测到13个显著的多效位点,挖掘到TraesCS4A01G023100TraesCS1B01G294400TraesCS4A01G006200等16个可能与主胚根生长相关的候选基因,这些基因可能通过调控DNA拓扑结构异构酶、泛素结合酶E2、磷酸肌醇磷酸酶家族蛋白等参与小麦主胚根系的建成。本研究结果为小麦根系调控网络构建,以及优化根系构型和发挥根系功能提供了参考。

    Abstract:

    In order to explore the genetic mechanism of wheat seminal root formation and growth, 198 wheat natural cultivars growing in the Huang-Huai Area were investigated in this study. The total first grade branch number, branch density, length, surface area, volume and average diameter of the radicle were examined using 21 days old seedlings in the hydroponic culture. Q+K mixed linear model, in conjugation with the genotyping results by the 660K gene chip, was used to conduct genome-wide association analysis (GWAS) at radicle traits, followed by functional annotation and candidate gene mining of significant and repeatedly-detected association sites. At the six radicle growth traits, a continuous and normal or nearly normal distribution were observed, with coefficient of variation of 5.56%-22.10%. A total of 136 significant association sites were detected. They were distributed on the chromosomes except 7B, each of which could explain 5.10%-13.60% of phenotypic variation. Thirteen significant pleiotropic sites were identified from those 136 sites. Based on the annotation, 16 candidate genes that may be related to radicle growth were found, such as TraesCS4A01G023100TraesCS1B01G294400TraesCS4A01G006200. These genes were proposed to be involved in the formation of wheat radicle root system by regulating DNA topoisomerase, ubiquitin-conjugating enzyme E2, phosphoinositide phosphatase family protein and so on. The results of this study provided a reference for the construction of wheat root regulation network, as well as the optimization of root architecture and function.

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引用本文

杨键,梁文宪,王春艳,等.小麦主胚根生长及主要性状全基因组关联分析[J].植物遗传资源学报,2024,25(4):533-543.

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历史
  • 收稿日期:2023-10-08
  • 最后修改日期:2023-11-02
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  • 在线发布日期: 2024-04-29
  • 出版日期: 2024-04-16
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