1.江汉大学生命科学学院/湖北省汉江流域特色生物资源保护开发与利用工程技术研究中心,武汉430056;2.华中农业大学植物科学技术学院/国家油菜工程技术研究中心,武汉 430070
主要从事特色植物资源开发与利用研究,E-mail:xty99100911@163.com
曾长立,主要从事植物逆境生理、珍稀濒危植物保护与利用研究, E-mail:zengchangli@jhun.edu.cn
万何平,主要从事油菜耐盐碱遗传研究,E-mail:wanheping@jhun.edu.cn
生物育种专项(2022ZD04010);国家自然科学基金(U22A20469 );国家重点研发计划项目(2016YFD0100202-25)
1.College of Life Sciences, Jianghan University/Hubei Engineering Research Center for Conservation, Development and Utilization of Characteristic Biological Resources in Hanjiang River Basin, Wuhan 430056;2.College of Plant Science and Technology, Huazhong Agricultural University /National Oilseed Rape Engineering Technology Research Center, Wuhan 430070
Foundation projects: Biological Breeding Special (2022ZD04010);The National Natural Science Foundation of China (U22A20469); The National Key Research and Development Program (2016YFD0100202-25)
对286 份甘蓝型油菜品系进行苗期耐盐碱性鉴定,通过水培试验,测定盐碱胁迫处理下叶片数、绿叶数、绿叶比、株高、根长、根重等指标,通过盐碱胁迫综合评价值(D值)、极端材料筛选分析、相关性、主成分、隶属函数、频数分析和逐步回归分析法,对不同基因型的油菜种质建立苗期耐盐碱性综合评价模型并筛选出适宜的评价鉴定指标。盐碱胁迫下,叶片数与株高呈负相关,两者的相关系数未达到显著性,其他性状之间均呈正相关并达到了显著或极显著水平。利用主成分分析法将 7个耐盐碱指标综合为4个主成分,可代表油菜耐盐碱性88.349%的原始数据信息量。依据4个主成分的相对重要性(权重)进行加权,得到不同基因型的耐盐碱性综合评价值(D值)。结合隶属函数分析和极端材料筛选分析,筛选出4份耐碱盐的甘蓝型油菜种质和4份盐碱敏感种质。逐步回归分析结果表明,在油菜苗期测定其绿叶数、绿叶比、地上重、根长和根重,通过回归方程估算其D值,可以初步判断甘蓝型油菜种质资源耐盐碱性。
The salt-alkali tolerance of 286 brassica rapeseed strains at the seedling stage was identified in this study. The indexes of leaves number, green leaves number, green leaves rate,shoot length, root length, and root weight under salt-alkali stress were determined through a hydroponics test. Various methods including comprehensive assessment value (D-value), extreme material screening analysis, correlation analysis, principal component analysis, membership function analysis, frequency analysis and stepwise regression analysis were used. A comprehensive evaluation model for saline-alkali tolerance was established for different genotypes of rapeseed germplasm at the seedling stage and appropriate evaluation indexes were selected. Under saline-alkali stress conditions, leaves number showed a negative correlation with plant height but the correlation coefficient between them was not significant; however other traits showed positive correlations that reached a significant or extremely significant level. Principal component analysis was employed to synthesize 7 salt-alkali tolerance indexes into 4 principal components which could represent 88.349% of the original data information on rape′s salt-alkali tolerance. Based on the relative importance (weight) of these four principal components, a comprehensive evaluation value (D-value) for salt and alkali tolerance among different genotypes was obtained. Four alkali-salt-tolerant brassica napus germplasms and four saline-sensitive germplasms were screened using membership function analysis and extreme material screening analysis respectively. The results from stepwise regression analysis indicated that salinity tolerance in brassica napus germplasm resources could be determined by measuring the green leaves number, green leaves rate, shoot weight as well as root length and root weight at the seedling stage while estimating D-value using regression equations.
薛天源,鲁金春子,何思晓,等.