2025年4月4日 7:19 星期五
首页 > 过刊浏览>2024年第25卷第4期 >612-621. DOI:10.13430/j.cnki.jpgr.20230923001
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辣椒细胞核雄性不育材料GMS702AB的鉴定
作者:
作者单位:

1.安徽科技学院农学院，凤阳 233100;2.安徽省农业科学院园艺研究所/园艺作物种质资源创制及高效栽培安徽省重点实验室/ 农业农村部园艺作物种质创制与利用重点实验室(部省共建)，合肥 230031;3.安徽农业大学园艺学院，合肥 230036

作者简介:

研究方向为园艺作物育种栽培，E-mail：2091356882@qq.com

通讯作者:

孙玉军，研究方向为园艺作物逆境生理，E-mail：sunyujun208@163.com
江海坤，研究方向为蔬菜遗传育种，E-mail：jhk211@163.com

基金项目:

财政部和农业农村部国家现代农业产业技术体系（CARS-23-G40，CARS-23-G49）；国家重点研发计划（2023YFD1201500）；安徽省农业科学院青年英才经费（QNYC-202121）；安徽省科技重大专项（202203a06020030）；安徽省蔬菜产业技术体系专项（皖农科函［2021］711号）；安徽省辣椒良种联合攻关项目


Identification of Nuclear Male Sterile Material GMS702AB in Pepper
Author:
  • WANG Sheng 1,2

    WANG Sheng

    College of Agriculture， Anhui Science and Technology University ， Fengyang 233100;Institute of Horticulture， Anhui Academy of Agricultural Sciences / Anhui Provincial Key Laboratory for Germplasm Resources Creation & High-Efficiency Cultivation of Horticultural Crops/Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops， Ministry of Agriculture and Rural Affairs （Co-comstruction by Ministry and Province）， Hefei 230031
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  • JIA Li 2

    JIA Li

    Institute of Horticulture， Anhui Academy of Agricultural Sciences / Anhui Provincial Key Laboratory for Germplasm Resources Creation & High-Efficiency Cultivation of Horticultural Crops/Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops， Ministry of Agriculture and Rural Affairs （Co-comstruction by Ministry and Province）， Hefei 230031
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  • TANG Jing 2,3

    TANG Jing

    Institute of Horticulture， Anhui Academy of Agricultural Sciences / Anhui Provincial Key Laboratory for Germplasm Resources Creation & High-Efficiency Cultivation of Horticultural Crops/Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops， Ministry of Agriculture and Rural Affairs （Co-comstruction by Ministry and Province）， Hefei 230031;College of Horticulture， Anhui Agricultural University， Hefei 230036
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  • LI Haoyu 1,2

    LI Haoyu

    College of Agriculture， Anhui Science and Technology University ， Fengyang 233100;Institute of Horticulture， Anhui Academy of Agricultural Sciences / Anhui Provincial Key Laboratory for Germplasm Resources Creation & High-Efficiency Cultivation of Horticultural Crops/Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops， Ministry of Agriculture and Rural Affairs （Co-comstruction by Ministry and Province）， Hefei 230031
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  • SONG Tingting 2

    SONG Tingting

    Institute of Horticulture， Anhui Academy of Agricultural Sciences / Anhui Provincial Key Laboratory for Germplasm Resources Creation & High-Efficiency Cultivation of Horticultural Crops/Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops， Ministry of Agriculture and Rural Affairs （Co-comstruction by Ministry and Province）， Hefei 230031
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  • YUAN Juanwei 2,3

    YUAN Juanwei

    Institute of Horticulture， Anhui Academy of Agricultural Sciences / Anhui Provincial Key Laboratory for Germplasm Resources Creation & High-Efficiency Cultivation of Horticultural Crops/Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops， Ministry of Agriculture and Rural Affairs （Co-comstruction by Ministry and Province）， Hefei 230031;College of Horticulture， Anhui Agricultural University， Hefei 230036
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  • YAN Congsheng 2

    YAN Congsheng

    Institute of Horticulture， Anhui Academy of Agricultural Sciences / Anhui Provincial Key Laboratory for Germplasm Resources Creation & High-Efficiency Cultivation of Horticultural Crops/Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops， Ministry of Agriculture and Rural Affairs （Co-comstruction by Ministry and Province）， Hefei 230031
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  • FANG Ling 2

    FANG Ling

    Institute of Horticulture， Anhui Academy of Agricultural Sciences / Anhui Provincial Key Laboratory for Germplasm Resources Creation & High-Efficiency Cultivation of Horticultural Crops/Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops， Ministry of Agriculture and Rural Affairs （Co-comstruction by Ministry and Province）， Hefei 230031
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  • ZHANG Qian 2

    ZHANG Qian

    Institute of Horticulture， Anhui Academy of Agricultural Sciences / Anhui Provincial Key Laboratory for Germplasm Resources Creation & High-Efficiency Cultivation of Horticultural Crops/Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops， Ministry of Agriculture and Rural Affairs （Co-comstruction by Ministry and Province）， Hefei 230031
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  • SUN Yujun 1

    SUN Yujun

    College of Agriculture， Anhui Science and Technology University ， Fengyang 233100
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  • JIANG Haikun 2

    JIANG Haikun

    Institute of Horticulture， Anhui Academy of Agricultural Sciences / Anhui Provincial Key Laboratory for Germplasm Resources Creation & High-Efficiency Cultivation of Horticultural Crops/Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops， Ministry of Agriculture and Rural Affairs （Co-comstruction by Ministry and Province）， Hefei 230031
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  • SUN Xueliang 2

    SUN Xueliang

    Institute of Horticulture， Anhui Academy of Agricultural Sciences / Anhui Provincial Key Laboratory for Germplasm Resources Creation & High-Efficiency Cultivation of Horticultural Crops/Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops， Ministry of Agriculture and Rural Affairs （Co-comstruction by Ministry and Province）， Hefei 230031
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  • ZHANG Tao 2

    ZHANG Tao

    Institute of Horticulture， Anhui Academy of Agricultural Sciences / Anhui Provincial Key Laboratory for Germplasm Resources Creation & High-Efficiency Cultivation of Horticultural Crops/Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops， Ministry of Agriculture and Rural Affairs （Co-comstruction by Ministry and Province）， Hefei 230031
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Affiliation:

1.College of Agriculture， Anhui Science and Technology University ， Fengyang 233100;2.Institute of Horticulture， Anhui Academy of Agricultural Sciences / Anhui Provincial Key Laboratory for Germplasm Resources Creation & High-Efficiency Cultivation of Horticultural Crops/Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops， Ministry of Agriculture and Rural Affairs (Co-comstruction by Ministry and Province)， Hefei 230031;3.College of Horticulture， Anhui Agricultural University， Hefei 230036

Fund Project:

Foundation projects： China Agriculture Research System of MOF and MARA（CARS-23-G40，CARS-23-G49）；The National Key Research and Development Program （ 2023YFD1201500）； The Second Level Youth Development Fund from Anhui Academy of Agricultural Sciences （QNYC-202121）；Important Science & Technology Specific Projects of Anhui Province（202203a06020030）； The Anhui Province Vegetable Industry Technology System （Anhui Agricultural Science Letter ［2021］ No.711）； Anhui Province Improved Variety Joint Research Project

  • 摘要
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    摘要:

    本研究发现1个具有雄性不育与单性结实特征的细胞核雄性不育系辣椒材料，并对该材料的农艺性状、单性结实坐果率、不同发育时期的不育系单性结实与可育系单性结实内源激素进行测定；利用田间鉴定和显微镜镜检，分析了F2群体的遗传分离情况，并利用辣椒细胞核雄性不育基因ms-3、msw、ms、msms、msc-1开发的分子标记，分析了群体的育性分离比。结果表明：不育系单性结实果实纵横径较大，可育系与不育系单性结实坐果率明显不同，可育系坐果率为22%，不育系坐果率为43%；不同时期不育系单性结实果实的赤霉素（GA4）含量显著高于可育系单性结实果实；田间鉴定和分子标记检测表明，F2群体中可育系有97株，不育系有30株，分离比为3.23∶1，符合孟德尔遗传规律，确定其育性由隐性单基因控制，将该辣椒细胞核雄性不育系命名为GMS702AB。本研究提供新的辣椒不育系，有助于辣椒育种和种子生产。

    Abstract:

    This study identified a nuclear male sterile pepper line showing male sterility and parthenocarpy characteristics， and the agronomic traits and parthenocarpy fruit setting rate of the material were determined， and the parthenocarpy of the sterile line at different developmental stages and the parthenocarpy of the fertile line were determined for endogenous hormones. In addition， the genetic segregation of F2 population was analyzed by field identification and microscopic examination， and the fertility segregation ratio of the population was analyzed by using the molecular markers developed by pepper nuclear male sterile genes ms-3， msw， ms， msms and msc-1.The results showed the vertical and horizontal diameters of the parthenocarpy fruit of the sterile line were larger， and the fruit setting rate of the fertile line and the sterile line was significantly different. The fruit setting rate of the fertile line was 22 %， and the fruit setting rate of the sterile line was 43%. The GA4 （gibberellin） content in the parthenocarpic fruit of the sterile line in different periods was significantly higher than that of the fertile line. There are 97 fertile lines and 30 sterile lines in the F2 population，with a separation ratio of 3.23 ： 1，which is consistent with the Mendelian inheritance law. It showsd that its fertility was controlled by a recessive single gene. We named the nuclear male sterile line of pepper as GMS702AB. This study provides a new nuclear male sterile line valuable in pepper breeding and seed production.

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汪胜,贾利,唐菁,等.辣椒细胞核雄性不育材料GMS702AB的鉴定[J].植物遗传资源学报,2024,25(4):612-621.

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  • 收稿日期:2023-09-23
  • 最后修改日期:2023-12-13
  • 在线发布日期: 2024-04-29
  • 出版日期: 2024-04-16
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