This study used the derivative line H103P of the wheat line Hussar， which has resistance to leaf rust， as the resistant parent and Zhengzhou 5389 as the susceptible parent to hybridize and obtain 234 F₄ family populations as materials for leaf rust resistance gene mapping analysis. Identification of leaf rust resistance during seedling stage using 16 physiological races of leaf rust fungus with different virulence. The results showed that Zhoumai 22 and vector varieties carrying Lr13， Lr23 and Lr16 single gene were susceptible to 16 physiological races of leaf rust. Except for one race （PHKT）， H103P showed resistance to all other races. The resistance of H103P is different from the vector varieties corresponding to these three genes （Lr13，Lr23 and Lr16）. Use 5 highly virulent mixed strains （THTT， PHTT^②， FHJS^②， PHKS， PHTT^①） for field identification of leaf rust resistance. The results showed that H103P， SAAR， Zhoumai 22 and Lr13 vector variety exhibited high resistance in the field. 234 F₄ family populations are distributed continuously and exhibit good resistance during the mature stage in the field. The results of leaf rust resistance gene mapping analysis indicate that H103P is located as a disease resistance gene on the 2BS chromosome of wheat， temporarily named LrHu. Using specific primers containing Lr13 for specific cleavage of H103P and amplification products of Zhengzhou 5389， it is showed that the wheat leaf rust resistance gene Lr13 is contained in H103P. The relationship between the wheat leaf rust resistance gene LrHu and Lr13 is needed to verify by the further research.