2025年5月21日 8:55 星期三
首页 > 过刊浏览>2024年第25卷第8期 >1268-1282. DOI:10.13430/j.cnki.jpgr.20231106003
PDF HTML阅读 XML下载 导出引用 引用提醒
小麦抗穗发芽种质鉴评及其初步应用
作者:
作者单位:

1.江苏徐淮地区淮阴农业科学研究所，淮安 223001;2.江苏省粮食作物现代产业技术协同创新中心，扬州 225009;3.生物育种钟山实验室 ，南京 210014

作者简介:

研究方向为作物遗传育种，E-mail ： panly89@126.com

通讯作者:

孙苏阳，研究方向为小麦高效育种，E-mail ： ssy6688@163.com

基金项目:

江苏省农业科技自主创新资金项目（CX（21）3104）；淮安市农业科学研究院发展基金（HNY202007）；淮安市农业科学研究院高层次引进人才科研启动发展基金项目（0052023016B）；江苏省种业振兴“揭榜挂帅”项目（JBGS［2021］051）


Evaluation of Wheat Pre-Harvest Sprouting Resistant Germplasm Resources and Their Preliminary Application
Author:
  • PAN Liyuan 1,2,3

    PAN Liyuan

    Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu，Huaian 223001;Jiangsu Provincial Collaborative Innovation Center of Modern Industrial Technology for Grain Crops， Yangzhou 225009;Zhongshan Laboratory of Biological Breeding， Nanjing 210014
    在期刊界中查找
    在百度中查找
    在本站中查找
  • WANG Yongjun 1,2,3

    WANG Yongjun

    Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu，Huaian 223001;Jiangsu Provincial Collaborative Innovation Center of Modern Industrial Technology for Grain Crops， Yangzhou 225009;Zhongshan Laboratory of Biological Breeding， Nanjing 210014
    在期刊界中查找
    在百度中查找
    在本站中查找
  • LI Haijun 1,2,3

    LI Haijun

    Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu，Huaian 223001;Jiangsu Provincial Collaborative Innovation Center of Modern Industrial Technology for Grain Crops， Yangzhou 225009;Zhongshan Laboratory of Biological Breeding， Nanjing 210014
    在期刊界中查找
    在百度中查找
    在本站中查找
  • HOU Fu 1,2,3

    HOU Fu

    Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu，Huaian 223001;Jiangsu Provincial Collaborative Innovation Center of Modern Industrial Technology for Grain Crops， Yangzhou 225009;Zhongshan Laboratory of Biological Breeding， Nanjing 210014
    在期刊界中查找
    在百度中查找
    在本站中查找
  • LI Jing 1,2,3

    LI Jing

    Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu，Huaian 223001;Jiangsu Provincial Collaborative Innovation Center of Modern Industrial Technology for Grain Crops， Yangzhou 225009;Zhongshan Laboratory of Biological Breeding， Nanjing 210014
    在期刊界中查找
    在百度中查找
    在本站中查找
  • LI Lili 1,2,3

    LI Lili

    Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu，Huaian 223001;Jiangsu Provincial Collaborative Innovation Center of Modern Industrial Technology for Grain Crops， Yangzhou 225009;Zhongshan Laboratory of Biological Breeding， Nanjing 210014
    在期刊界中查找
    在百度中查找
    在本站中查找
  • SUN Suyang 1,2,3

    SUN Suyang

    Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu，Huaian 223001;Jiangsu Provincial Collaborative Innovation Center of Modern Industrial Technology for Grain Crops， Yangzhou 225009;Zhongshan Laboratory of Biological Breeding， Nanjing 210014
    在期刊界中查找
    在百度中查找
    在本站中查找
Affiliation:

1.Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu，Huai'an 223001;2.Jiangsu Provincial Collaborative Innovation Center of Modern Industrial Technology for Grain Crops， Yangzhou 225009;3.Zhongshan Laboratory of Biological Breeding， Nanjing 210014

Fund Project:

Foundation projects： Jiangsu Province Agricultural Science and Technology Autonomous Innovation Fund Project （CX（21）3104）； Development Fund of Huai'an Institute of Agricultural Science （HNY202007）； Scientific Research Development of High-level Talents Introduced by Huai'an Institute of Agricultural Science （0052023016B）； Jiangsu Province Seed Industry Revitalization "Unveiling the List of Commanding Officers" Project （JBGS［2021］051）

  • 摘要
    • | |
  • 访问统计
    • |
  • 参考文献
    • |
  • 相似文献
    • |
  • 引证文献
    • | |
  • 文章评论
    摘要:

    小麦穗发芽会造成小麦产量和品质的显著降低，近年来在黄淮地区逐渐加重，威胁我国、特别是黄淮区域小麦生产安全，抗性种质及功能分子标记的筛选和利用是减轻穗发芽危害的根本途径。本研究以多年田间自然鉴定的77份抗穗发芽种质及128份导入了6个抗穗发芽种质的轮回群体创制的高代品系为材料，采用整穗发芽法和籽粒发芽法对穗发芽抗性进行鉴定和评价，明确穗发芽功能标记Vp1B3、Dorm-B1和PM19在抗性种质中的分布并评估其育种效果。结果表明种质资源中49.35%（38份）达到中抗水平，其中仅57.89%（22份）含有Vp1Ba或（和）Dorm-B1b抗性位点，西农172、Kalango、淮麦40、豫农186同时含有2个抗性位点；高代品系中36.72%（47份）达到中抗以上，其中87.23%含有抗性位点，17.02%（8份）同时含有2个抗性位点。抗性位点的累加可提升抗性水平。种质资源中不含有抗性位点的穗发芽率和发芽指数分别为36.65%和34.99%，而含有2个抗性位点的分别为18.17%和23.87%，高代材料也呈现相同的趋势。高代材料中达到中抗及以上水平的材料与其他材料之间含有的抗性位点数目存在显著差异，其中17.02%的中抗水平材料含有2个抗性位点，而未达到中抗水平的其他材料中仅有4.94%含有2个抗性位点，表明利用优异等位变异可显著改良抗性。本研究结合分子标记和表型鉴定进行抗穗发芽种质筛选，通过轮回选择进行种质创新，有望提升黄淮麦区的小麦穗发芽抗性水平。

    Abstract:

    Wheat pre-harvest sprouting significantly reduces yield production and flour quality. It has been gradually aggravated in recent years， and destabilize the safety of wheat production in China， particularly in the Huanghuai region. Identification of pre-harvest sprouting resistant germplasm and functional molecular markers are of importance to accelerate pre-harvest sprouting resistant breeding. In this study， 77 pre-harvest sprouting resistant germplasms observed from field experiments and 128 advanced breeding lines from a rotational population introduced with six resistant germplasms were used. These genotypes were tested for pre-harvest sprouting resistance using the whole-split germination and seed germination methods， as well as genotyped by functional markers Vp1B3， Dorm-B1， and PM19. 49.35% （38 of 77） of germplasm resources showed intermediate resistance， and 57.90%（22） of them contained functional resistance alleles of Vp1Ba or（and） Dorm-B1b. The germplasm resources Xinong 172， Kalango， Huaimai 40 and Yunong 186 were detected carrying both two functional alleles. 36.72% （47） of the advanced breeding lines showed intermediate resistance， of which 87.23% contained resistance alleles and 17.02% （8） contained two functional alleles. Stacking resistance loci can increase the level of pre-harvest sprouting resistance. In the germplasm resources， the whole-split germination and seed germination decreased from 36.65% and 34.99% in non-resistant individuals， respectively， to 18.17 % and 23.87 % in individuals with two resistance loci. The same pattern has been observed in advanced lines. There was a significant difference in the number of resistant loci between advanced materials and others. Among them， 17.02% of the materials with medium resistance level contained two resistance loci， while only 4.94% of the other materials without medium resistance level contained two resistance loci. This study deployed molecular markers and phenotypic characterization techniques to identify pre-harvest sprouting resistance germplasm， followed by germplasm innovation using dwarf failing rotation selection， which provided a basis for future improvement of wheat spike germination resistance in the Huanghuai region in China.

    参考文献
    相似文献
    引证文献
引用本文

潘丽媛,王永军,李海军,等.小麦抗穗发芽种质鉴评及其初步应用[J].植物遗传资源学报,2024,25(8):1268-1282.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2023-11-06
  • 在线发布日期: 2024-08-09
文章二维码
您是第位访问者
ICP:京ICP备09069690号-23
京ICP备09069690号-23
植物遗传资源学报 ® 2025 版权所有
技术支持：北京勤云科技发展有限公司
请使用 Firefox、Chrome、IE10、IE11、360极速模式、搜狗极速模式、QQ极速模式等浏览器，其他浏览器不建议使用!