小麦种质资源穗发芽抗性鉴定和分子标记分析
作者:
作者单位:

1.河南农业大学农学院/省部共建小麦玉米作物学国家重点实验室/河南粮食作物协同创新中心/国家小麦工程技术研究中心,郑州 450001;2.河南丰德康种业股份有限公司,郑州 450001;3.河南省焦作市种子站,焦作 454000

作者简介:

研究方向为小麦种质资源穗发芽抗性鉴定,E-mail:13033736612@163.com;

通讯作者:

殷贵鸿,研究方向为小麦遗传育种,E-mail:yinguihong2008@163.com
焦竹青,研究方向为小麦种业,E-mail:Jzszzzglk@163.com

中图分类号:

基金项目:

2022年度河南省重大科技专项(221100110700);河南省农业良种联合攻关项目(2022010102);河南省现代农业产业技术体系建设(HARS-22-01-G2);河南省科技研发计划联合基金(222103810002);2022年度河南省产业研发联合基金重大项目(225101610048);河南农业大学拔尖人才项目(30500678)


Preharvest Sprouting Resistance Test and Functional Markers Assisted Genotyping in Wheat Germplasm Resources
Author:
Affiliation:

1.College of Agriculture,Henan Agricultural University/Co-construction State Key Laboratory of Wheat and Maize Crop Science/ Collaborative Innovation Center of Henan Grain Crops/The National Engineering Research Centre for Wheat,Zhengzhou 450001;2.Henan Fengdekang Seed Industry Co., Ltd, Zhengzhou 450001;3.Henan Jiaozuo Seed Station, Jiaozuo 454000

Fund Project:

Foundation projects: Major Science and Technology Project of Henan Province in 2022(221100110700); Henan Province Agricultural Improved Breeds Joint Research Project (2022010102); Modern Agricultural Industry Technology System Construction Project of Henan Province (HARS-22-01-G2); Henan Provincial Science and Technology R&D Plan Joint Fund (222103810002); Major Project of Henan Province Industrial Research and Development Joint Fund in 2022 (225101610048); Top Talent Project of Henan Agricultural University (30500678)

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    摘要:

    为了鉴定小麦种质资源的穗发芽抗性,筛选出有效的抗穗发芽分子标记,进而挖掘优异白粒小麦抗穗发芽种质资源,本研究通过整穗发芽试验对222份小麦种质资源进行穗发芽抗性鉴定,并利用myb10DDFR-BVp1B3PM19-A1MFT-3AMFT-A2MKK3-AQSD1等8个抗穗发芽基因的功能分子标记对供试材料进行基因型检测。表型鉴定结果表明,222份小麦种质资源材料的相对穗发芽率存在显著差异,相对穗发芽率变化范围为0~1.15,平均相对穗发芽率为0.73,鉴定出抗穗发芽小麦材料38份,其中包括白粒小麦9份、红粒小麦27份、黑粒小麦2份。等位基因类型与相对穗发芽率相关性分析表明,相对穗发芽率与功能标记myb10DDFR-BVp1B3MFT-3AMFT-A2呈极显著相关,而与PM19-A1MKK3-AQSD1相关性不显著,说明myb10DDFR-BVp1B3MFT-3AMFT-A2等分子标记可用作小麦抗穗发芽基因型检测和分子标记辅助育种。综合表现型和基因型结果,筛选出豫农914、豫农946、丰德存麦30、泛麦5号、徐麦029、连麦1901、保丰1903、郑麦829、13网27-8等9份抗穗发芽白粒小麦种质资源,可用于小麦抗穗发芽遗传育种和抗穗发芽品种布局。

    Abstract:

    This study aims to identify the pre-harvest sprouting resistance in wheat germplasm resources and molecular markers for marker-assisted selection, ultimately obtaining excellent white grain wheat germplasm resources applicable in wheat breeding. These spikes of 222 wheat germplasm resources were tested for pre-harvest sprouting resistance, and the functional markers of eight anti-pre-harvest sprouting genes, including myb10DDFR-BVp1B3PM19-A1MFT-3AMFT-A2MKK3-AQSD1, were used for genotyping. Phenotypic identification results showed that there were significant differences in the relative sprouting percentage of 222 wheat germplasm resources. The relative sprouting percentage ranged from 0 to 1.15, and the average relative sprouting percentage was 0.73. 38 wheat materials were identified to be resistant to pre-harvest sprouting, including 9 white wheat, 27 red wheat and 2 black wheat. Correlation analysis between allele types and relative sprouting percentage showed that the relative sprouting percentage was significantly correlated with functional markers myb10DDFR-BVp1B3MFT-3A and MFT-A2, but not with PM19-A1MKK3-A and QSD1. These results indicated that molecular markers myb10DDFR-BVp1B3MFT-3A and MFT-A2 could be used for detection of pre-harvest sprouting resistance and marker-assisted breeding. Based on the phenotype and genotype results, nine white wheat germplasm resources with pre-harvest sprouting resistance were selected, including Yunong 914, Yunong 946, Fengdecunmai 30, Fangmai 5, Xumai 029, Lianmai 1901, Baofeng 1903, Zhengmai 829 and 13wang27-8, which could be used for genetic breeding and layout of wheat varieties with pre-harvest sprouting resistance.

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龚承儒,袁雨豪,刘振,等.小麦种质资源穗发芽抗性鉴定和分子标记分析[J].植物遗传资源学报,2024,25(9):1493-1503.

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  • 收稿日期:2023-11-13
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  • 在线发布日期: 2024-09-02
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