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首页 > 过刊浏览>2024年第25卷第9期 >1524-1539. DOI:10.13430/j.cnki.jpgr.20231117001
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小麦B-ARR转录因子家族基因鉴定与表达模式分析
作者:
作者单位:

1.山西农业大学农学院，太原 030031;2.山西农业大学生命科学学院，太原 030031

作者简介:

研究方向为作物分子设计育种，E-mail：wcbksl@126.com；

通讯作者:

唐朝晖，研究方向为小麦种质资源创制，E-mail：tzhhui126@126.com

基金项目:

国家重点研发计划项目（2022YFD1200901）；山西省重点研发计划项目（202102140601001）；山西省现代农业产业技术体系建设专项资金（2023CYJSTX02-06）


Genome Identification and Expression Analysis of B-ARR Transcription Factors Family in Wheat
Author:
Affiliation:

1.College of Agricultural， Shanxi Agricultural University， Taiyuan 030031;2.College of Life Sciences， Shanxi Agricultural University， Taiyuan 030031

Fund Project:

Foundation projects： National Key R&D Program of China（2022YFD1200901）； Shanxi Provincial Key R&D Program（202102140601001）； Shanxi Province Modern Agricultural Industry Technology System Construction Special Fund （2023CYJSTX02-06）

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    摘要:

    B型反应调节因子（B-ARR， type-B authentic response regulator）家族基因是细胞分裂素信号转导的正向调节因子，在植物生长发育和抗非生物胁迫中起重要作用。然而，目前对小麦中B-ARR基因家族的研究还很少。本研究从小麦基因组中鉴定出25个B-ARR基因家族成员，利用生物信息学方法分析其理化性质、基因结构、顺式作用元件和非生物胁迫诱导的表达模式。结果表明，B-ARR蛋白都定位于细胞核中，B-ARR蛋白二级结构以α螺旋和无规则卷曲为主要结构。B-ARR基因在小麦染色体上分布不均匀，其中7号染色体上含有的B-ARR基因数量最多。此外，在B-ARR的启动子区域中鉴定出了与生长发育、激素响应以及生物和非生物胁迫相关的多种顺式作用调节元件。RT-qPCR 分析表明，基因TaARRM-like9、TaARRM-like10、TaARRM-like12和TaARRM-like13在干旱、盐和低温胁迫下表达量显著上调，能够响应非生物胁迫。本研究为进一步研究B-ARR转录因子在小麦发育和非生物胁迫应答中的作用奠定了基础。

    Abstract:

    The type-B authentic response regulator （B-ARR） family members are positive regulators in cytokinin signal transduction， and play important roles in plant growth and development and resistance to abiotic stresses. However， there are few studies on the B-ARR gene family in wheat. In this study， 25 B-ARR gene family members were identified from wheat genome， and their physicochemical properties， gene structure， cis-acting elements and abiotic stress-induced expression patterns were analyzed by bioinformatics methods. The results showed that all B-ARR proteins were localized in the nucleus based on bioinformatics prediction， and their secondary structure was mainly consisting of α-helix and random crimp. B-ARR genes were not evenly distributed on wheat chromosomes， and the number of B-ARR genes was the highest on chromosome 7. In addition， multiple cis-acting regulatory elements related to growth and development， hormone response， and biological and abiotic stress have been identified in the promoter regions. RT-qPCR analysis showed that the relative expression of TaARRM-like9， TaARRM-like10， TaARRM-like12 and TaARRM-like13 were significantly up-regulated under abiotic stresses treatments， including drought， salt and low temperature. This study laid a foundation for further research on the role of B-ARR transcription factor in wheat development and abiotic stress response.

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王长彪,尹誉蓉,程泽,等.小麦B-ARR转录因子家族基因鉴定与表达模式分析[J].植物遗传资源学报,2024,25(9):1524-1539.

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  • 收稿日期:2023-11-17
  • 在线发布日期: 2024-09-02
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