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首页 > 过刊浏览>2024年第25卷第9期 >1573-1588. DOI:10.13430/j.cnki.jpgr.20231125001
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野生大豆与栽培大豆几丁质酶基因鉴定及其表达分析
作者:
作者单位:

河北农业大学农学院/华北作物改良与调控国家重点实验室/华北作物种质资源研究与利用教育部重点实验室/ 河北省作物种质资源重点实验室，保定 071001

作者简介:

研究方向为大豆遗传育种与分子生物学，E-mail： zhanghua0316@163.com

通讯作者:

李喜焕，研究方向为大豆遗传育种与分子生物学，E-mail ： lixihuan@hebau.edu.cn
张彩英，研究方向为大豆遗传育种与分子生物学，E-mail： zhangcaiying@hebau.edu.cn

基金项目:

河北省现代农业产业技术体系建设专项资金（HBCT2024060204）


Identification and Expression Analysis of Chitinase Genes in Wild and Cultivated Soybeans
Author:
  • ZHANG Hua

    ZHANG Hua

    College of Agronomy， Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation / North China Key Laboratory for Crop Germplasm Resources of Education Ministry / Hebei Key Laboratory of Crop Germplasm Resources，Baoding 071001
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  • LI Na

    LI Na

    College of Agronomy， Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation / North China Key Laboratory for Crop Germplasm Resources of Education Ministry / Hebei Key Laboratory of Crop Germplasm Resources，Baoding 071001
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  • XING Xinzhu

    XING Xinzhu

    College of Agronomy， Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation / North China Key Laboratory for Crop Germplasm Resources of Education Ministry / Hebei Key Laboratory of Crop Germplasm Resources，Baoding 071001
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  • SHAO Zhenqi

    SHAO Zhenqi

    College of Agronomy， Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation / North China Key Laboratory for Crop Germplasm Resources of Education Ministry / Hebei Key Laboratory of Crop Germplasm Resources，Baoding 071001
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  • LI Xihuan

    LI Xihuan

    College of Agronomy， Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation / North China Key Laboratory for Crop Germplasm Resources of Education Ministry / Hebei Key Laboratory of Crop Germplasm Resources，Baoding 071001
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  • ZHANG Caiying

    ZHANG Caiying

    College of Agronomy， Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation / North China Key Laboratory for Crop Germplasm Resources of Education Ministry / Hebei Key Laboratory of Crop Germplasm Resources，Baoding 071001
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Affiliation:

College of Agronomy， Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation / North China Key Laboratory for Crop Germplasm Resources of Education Ministry / Hebei Key Laboratory of Crop Germplasm Resources，Baoding 071001

Fund Project:

Foundation project： Hebei Agriculture Research System （HBCT2024060204）

  • 摘要
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    摘要:

    几丁质酶是以几丁质等为底物的糖基水解酶（GH，glycosyl hydrolases），在植物生长发育及抵御逆境中发挥重要功能。然而，大豆几丁质酶基因的组织表达模式及对逆境的响应尚不清楚，影响了其在遗传改良中的应用。本研究分别鉴定野生大豆与栽培大豆几丁质酶基因，并分析其表达模式。结果发现，野生大豆与栽培大豆分别含62个和55个几丁质酶基因，位于17条和18条染色体上；进化树分析发现，117个基因分为5类，其中类群III与类群V属于GH18亚家族，类群I、类群II与类群IV属于GH19亚家族；启动子分析发现，几丁质酶家族成员包含响应激素及逆境胁迫顺式作用元件。栽培大豆几丁质酶基因表达分析发现，其在不同组织及抗病耐逆等过程差异表达，其中Gm.01G142400和Gm.13G346700等在接种花叶病毒的抗病品种叶片中诱导表达，Gm.03G254300和Gm.20G164600等在低磷胁迫的磷高效品种根系中诱导表达，Gm.08G259200和Gm.19G245400等在低磷胁迫根瘤中诱导表达；野生大豆几丁质酶基因表达分析发现，其在不同组织及耐盐过程差异表达，其中Gs.02G002604和Gs.02G002940等在盐胁迫耐盐品种叶片中诱导表达。以上结果为挖掘利用几丁质酶基因奠定了基础。

    Abstract:

    Chitinase is a kind of glycosyl hydrolases （GH） which hydrolyzes the chitin and other polymers. Chitinase plays an important function in the plant growth and development， as well as in the resistant process to diverse stresses. However， the tissue expression patterns and responses to diverse stresses of chitinase genes in soybean are still unclear， which seriously limited its application in genetic improvement. In this study， the chitinase family genes were identified in the wild soybean （Glycine soja Sieb. and Zucc.） and cultivated soybean （Glycine max（L.）Merr.）， and the expression patterns were also analyzed. The results showed that 62 and 55 chitinase genes were identified in the wild soybean and cultivated soybean， which located on 17 and 18 chromosomes， respectively. The phylogenetic tree analysis showed that the chitinase genes were divided into five categories， with Class III and Class V belonging to the GH18 subfamily， while Class I， Class II and Class IV belonging to the GH19 subfamily. Further analysis found many cis-acting elements in the promoter regions of chitinase genes responding to various plant hormones and stresses.Further gene expression analyses in cultivated soybean showed that the chitinase genes presented differential expressions in different tissues and under different stress conditions. Among these genes， Gm.01G142400 and Gm.13G346700 were strongly induced in the leaves of resistant variety after soybean mosaic virus inoculation， Gm.03G254300 and Gm.20G164600 were induced in soybean roots after low phosphorus treatment， and Gm.08G259200 and Gm.19G245400 were induced in soybean nodules under low phosphorus condition. Gene expression analysis in wild soybean showed that the chitinase genes presented differential expressions in different tissues and after salt stress， among which Gs.02G002604 and Gs.02G002940 were highly induced in the leaves of tolerant variety after salt treatment. These results provide important references for further utilizing the chitinase genes in soybean genetic improvement.

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张华,李娜,邢馨竹,等.野生大豆与栽培大豆几丁质酶基因鉴定及其表达分析[J].植物遗传资源学报,2024,25(9):1573-1588.

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