广州大学生命科学学院/广东省植物适应性与分子设计重点实验室/分子遗传与进化创新研究中心,广州 510006
第一作者研究方向为植物资源利用,E-mail :2278865217@qq.com;王一帆为共同第一作者
李美娜,研究方向为大豆杂种优势利用和生物钟与环境互作,E-mail : limeina@gzhu.edu.cn
国家自然科学基金(32072084);国家重点研发计划项目(2021YFF1001200);广州市基础研究计划市校(院)联合资助基础与应用基础研究项目(202201020129);青年人才项目(RQ2020029);中国博士后科学基金(2022M710873)
School of Life Sciences, Guangzhou University/Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design/Innovative Center of Molecular Genetics and Evolution, Guangdong 510006
Foundation projects: National Natural Science Foundation of China (32072084); National Key Research and Development Program (2021YFF1001200); Guangzhou Basic Research Program Jointly Funded Basic and Applied Basic Research Project (202201020129); Youth Talent Project (RQ2020029); China Postdoctoral Science Foundation (2022M710873)
大豆(Glycine max (L.) Merr.)是自花授粉作物,通过人工去雄的办法生产杂交种,不仅繁琐且成本高。雄性不育基因功能的研究是大豆杂种优势利用的前提之一,大豆雄性不育位点报道较少,定位及功能研究进展缓慢。随着大豆转基因体系的成熟及生物技术的发展,利用反向遗传学研究大豆雄性不育基因的功能变得相对容易。本研究通过转录组数据分析发现大豆中编码小G蛋白的GmARFA1a受到大豆雄性育性控制基因MS1(Male Sterile 1)和MS2的调控,公共数据库数据表明GmARFA1a在大豆未开放的花中表达量最高,而qRT-PCR数据进一步明确GmARFA1a在大豆授粉前雄蕊中优势表达。花粉萌发实验及结实率统计发现Gmarfa1a突变体花粉活力下降导致结实率受到明显抑制。本研究对GmARFA1a基因功能进行了初步解析,明确其对大豆雄性育性存在一定影响。研究结果不仅丰富了对GmARFA1a乃至ARF基因家族成员功能的认识,也为后续深入研究大豆GmARFA1a功能及大豆杂种优势利用奠定了基础。
Soybean (Glycine max (L.) Merr.) is a self-pollinating crop, and producing hybrids through artificial emasculation is time-consuming and expensive. Unlocking the function of the male sterility gene is a prerequisite for harnessing heterosis in soybean. Up to now, only a few loci of male sterility had been reported in soybean, and progress in molecular cloning and functional characterization of related genes lagged behind. Advances in biotechnology and soybean genetic transformation system enabled the possibility to employ reverse genetics methodology for studying the male sterility genes. The transcriptomic data indicated that the small G protein encoding gene GmARFA1a was regulated simultaneously by the male sterility gene MS1 (Male Sterile 1) and MS2. Data from the public library indicated that GmARFA1a expression was the highest in unopened soybean flowers, qRT-PCR data demonstrated that GmARFA1a was preferentially expressed in stamen before flowering. The pollen germination experiment and seed setting statistics found that the decrease of pollen viability of Gmarfa1a mutant resulted in the significant inhibition of seed setting Collectively, this study identified the GmARFA1a gene and uncovered its function on male fertility. It will not only enhance our understanding of the GmARFA1a and ARF gene families but also lay the foundation for further study the function of GmARFA1a genes and the utilization of heterosis in soybean.
姚士恩,王一帆,王宁,等.大豆雄蕊优势表达基因