晚香玉鳞茎的全长转录组分析
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作者单位:

1.四川农业大学风景园林学院,成都 611130;2.四川天艺生态园林股份有限公司,成都 611130

作者简介:

研究方向为园林植物遗传育种,E-mail:bnjfx@126.com

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基金项目:

成都市高等院所人才创新服务项目(2021-RC03-00001-CG)


Full-length Transcriptome Analysis of Bulbs of Polianthes tuberose L.
Author:
Affiliation:

1.School of Landscape Architecture,Sichuan Agricultural University,Chengdu 611130;2.Sichuan Tianyi Ecological Landscape Co., Ltd, Chengdu 611130

Fund Project:

Foundation project: Chengdu Higher Education Institute Talent Innovation Service Project(2021-RC03-00001-CG)

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    摘要:

    晚香玉为石蒜科的多年生鳞茎花卉,是著名的香花植物,具有较高的观赏、经济和药用价值,鳞茎是其主要的繁殖器官和药用器官,为深入探析其生长发育的机制和次生代谢途径,利用高通量测序PacBio Sequel平台,以其鳞茎为材料,使用单分子长读数测序技术(SMART,single molecule long reading sequencing technology)对晚香玉鳞茎进行三代全长转录组测序及分析。平台共获得 508012条高质量环形一致序列 (CCS, circular consensus)和 402032 条全长非嵌合序列(FLNC, full-lenth non-chimeric read),对全长非嵌合序列进行聚类得到高质量一致序列共137215个,经质控和去冗余后共获得 81719个转录本(Unigene) 和36215个完整CDS区。获得的所有转录本经过NR、SwissProt、KOG、KEGG、GO等数据库进行注释和功能分类,结果共有64362个单基因被注释,NR注释的基因数量最多,为63538个,占98.72 %;KEGG注释的基因最少,58878个基因被注释到148 条途径,代谢途径分布的基因最多(11112)。同时分析出41240个SSR、15835个lncRNA和 4903个转录因子,RLK转录因子家族的基因最多;发现同源盒转录因子可能参与了晚香玉鳞茎的发育过程,并对其中的PtKNOX1L13基因进行了克隆,发现其在鳞茎的起始、膨大和成熟3个时期表达量均比较高,瞬时过量表达表明其能促进珠芽的发育,可能是参与鳞茎形成和发育的重要功能基因。本研究为深入研究晚香玉的重要功能基因及种质资源创新提供了参考和依据。

    Abstract:

    Polianthes tuberose L. is a perennial bulbous flower of the Amaryllidaceae family. It is a famous fragrant flower plant with high ornamental, economic and medicinal values. Bulbs are their main reproductive and medicinal organs. In order to further explore its growth and development mechanism and secondary metabolic pathways, we use the high-throughput sequencing PacBio Sequel platform to make use of its bulbs as materials, the single molecule long reading sequencing technology (SMART) was used to sequence and analyze the full-length transcriptome of Agave amica bulbs for three generations. The platform obtained a total of 508012 high-quality CCS reads and 402032 full-length non chimeric sequences (FLNC), and clustered the full-length non chimeric sequences to obtain a total of 137215 high-quality consistent sequences. After quality control and redundancy removal, a total of 81719 transcripts (Unigene) and 36215 complete CDS regions were obtained. All transcripts obtained were annotated and functionally classified through databases such as NR, SwissProt, KOG,KEGG GO, etc. The result showed that a total of 64362 single genes were annotated, with NR having the highest annotation count of 63538, accounting for 98.72%,while KEGG had the lowest number of annotated genes,with 58878 genes annotated to 148 pathways. And 41240 SSRs, 15835 lncRNAs and 4903 transcription factors were analyzed. RLK transcription factor family has the most genes. The discovery of homologous box transcription factors family may be involved in the development process of tuber, PtKNOX1L13 gene was cloned,its expression levels were relatively high during the initiation, enlargement, and maturity stages of tubers, transient overexpression indicates that it can promote the development of bulbils,so that it may be an important functional gene involved in bulb formation and development.This study provides reference and basis for in-depth research and development of molecular markers, important functional genes and germplasm resources innovation of Polianthes tuberose L.

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引用本文

姜福星,吴菁熙,朱枭,等.晚香玉鳞茎的全长转录组分析[J].植物遗传资源学报,2024,25(9):1589-1600.

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  • 收稿日期:2023-12-11
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  • 在线发布日期: 2024-09-02
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