玉米PDI基因家族鉴定和表达模式分析
作者:
作者单位:

1.哈尔滨师范大学生命科学与技术学院黑龙江省分子细胞遗传与遗传育种重点实验室,哈尔滨 150025;2.中国农业科学院作物科学研究所/作物分子育种国家工程实验室,北京100081

作者简介:

研究方向为植物遗传学,E-mail: wujinli1998@163.com

通讯作者:

郭长虹,研究方向为植物遗传学,E-mail: kaku3008@126.com

中图分类号:

基金项目:

国家自然科学基金青年科学基金项目(32001559);国家玉米产业技术体系(CARS-02-02)


Identification and Expression Pattern Analysis of PDI Gene Family in Maize
Author:
Affiliation:

1.Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University ,Harbin 150025;2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/ National Engineering Laboratory for Crop Molecular Breeding,Beijing,100081

Fund Project:

Foundation projects: National Natural Science Foundation of China Youth Science Foundation Project (32001559);Modern Agro-Industry Research System of Maize(CARS-02-02)

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    摘要:

    蛋白质二硫键异构酶(PDI,protein disulfide isomerase)作为硫氧还蛋白家族的一员,是一种氧化还原酶,广泛存在于动物、植物及微生物中。在植物中,蛋白质二硫键的形成和异构主要由二硫键蛋白催化完成,在参与蛋白质正确组装折叠方面至关重要。本研究以拟南芥PDI家族基因序列信息为基础,利用同源序列比对方法,从玉米中鉴定出21个PDI基因,除8号染色体外,非均匀地分布在其余玉米9条染色体上。进化树分析表明,玉米PDI基因家族分为4个进化分支11个发育组,同一发育组的基因结构和保守基序相似。顺式作用元件分析表明,PDI基因家族启动子含响应逆境胁迫、植物激素及胚乳特异表达的顺式作用元件。表达模式分析表明,PDI基因家族在胚、胚乳和籽粒中表达量较高,在授粉后10 ~20 d的胚乳中表达量呈下降趋势,然后表达量又呈现上升趋势。生物多样性分析发现:ZmPDIL1-1基因存在多样性。亚细胞定位结果表明ZmPDIL1-1定位在内质网。本研究结果为玉米PDI家族基因功能研究提供参考。

    Abstract:

    Protein Disulfide Isomerase (PDI), a member of the thioredoxin superfamily, is an oxidoreductase widely exists in animals, plants and microorganisms. In plants, the formation and isomerization of protein disulfide bonds, which are mainly catalyzed by disulfide bond proteins, are crucial in the correct assembly and folding of proteins. In this study, based on the sequence similarity to the PDI gene family in Arabidopsis thaliana, 21 PDI genes were identified and found on nine chromosomes of maize. The phylogenetic tree analysis showed that the maize PDI gene family members were divided into four evolutionary branches and 11 developmental groups, and within each group the gene structure and conserved motifs were similar. The analysis of cis-acting elements in the promoters identified cis-acting elements associating to stress, plant hormones and endosperm specific expression. By quantifying the transcriptional profiles, the high expression was detected in embryo, endosperm and grain. In endosperm, a downward trend followed by upward from 10 to 20 days after pollination was observed. Biodiversity analysis revealed that was diversity of ZmPDIL1-1 gene. The result of subcellular localization showed that ZmPDIL1-1 was located to the endoplasmic reticulum. Collectively, this study provided reference for the functional analysis of PDI gene family in maize.

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引用本文

吴金丽,汤泽洋,鲁鑫,等.玉米PDI基因家族鉴定和表达模式分析[J].植物遗传资源学报,2024,25(1):97-110.

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  • 收稿日期:2023-06-21
  • 最后修改日期:2023-07-20
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  • 在线发布日期: 2024-01-08
  • 出版日期: 2024-01-08
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