高粱株高相关基因SbPH11分子标记的开发和应用
作者:
作者单位:

1.山西农业大学高粱研究所/省部共建有机旱作农业国家重点实验室(筹),晋中 030600;2.山西农业大学农学院,太谷 030801;3.中国科学院植物研究所,北京 100093;4.青海牛必乐农牧科技有限公司,西宁 810003;5.青海省饲草料技术推广站,西宁 810003

作者简介:

研究方向为作物遗传育种,E-mail:dgq0928@163.com

通讯作者:

平俊爱,研究方向为高粱遗传育种,E-mail:pingja1029@163.com

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基金项目:

西宁市重点研发与转化计划(2022-Y-18);省部共建有机旱作农业国家重点实验室自主研发项目(202002-4);中国科学院战略性先导科技专项(XDA26030201);财政部和农业农村部:国家现代农业产业技术体系(CARS-06)


Development and Application of Molecular Markers of Sorghum Plant Height Related Gene SbPH11
Author:
Affiliation:

1.Sorghum Research Institute, Shanxi Agricultural University/State Key Laboratory of Sustainable Dryland Agriculture (in preparation), Jinzhong 030600;2.College of Agriculture, Shanxi Agricultural University, Taigu 030801;3.Institute of Botany, Chinese Academy of Sciences, Beijing 100093;4.Qinghai Cattle Pierer Husbandry Technology Co.Ltd., Xining 810003;5.Qinghai Feed and Forage Technology Promotion Station, Xining 810003

Fund Project:

Foundation projects: The Key Research and Development Program of Xining(2022-Y-18);The Research Program Sponsored by State Key Laboratory of Sustainable Dryland Agriculture (202002-4);The Strategic Priority Research Program of the Chinese Academy of Sciences(XDA26030201); China Agriculture Research System of MOF and MARA(CARS-06)

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    摘要:

    生物量是饲用高粱的重要性状,株高与生物量呈正相关性。本研究以237份高粱自交系关联群体为材料,筛选到株高关联基因SbPH11的两个功能性SNP位点,两个SNP位点组合的单倍型共3种:SbPH11-Hap1、SbPH11-Hap2和SbPH11-Hap3,SbPH11-Hap2 所对应的高粱材料的株高极显著高于SbPH11-Hap1和SbPH11-Hap3所对应的高粱材料的株高,SbPH11-Hap1的高粱材料株高极显著高于SbPH11-Hap3的高粱材料株高。针对SbPH11的两个功能性SNP位点开发了KASP分子标记,利用该标记对30份高粱种质资源进行了基因分型和表型验证,结果证实开发的KASP分子标记可以准确地鉴定出SbPH11两个功能性SNP位点的基因型。该KASP分子标记可高效准确地预测不同高粱种质资源的株高类型,可应用于高粱株高的早期筛选和分子标记辅助选择育种。

    Abstract:

    Biomass is an important trait in forage sorghum, and positively correlates with plant height. In this study, two functional SNPs of the plant height associated gene SbPH11 were screened from 237 sorghum inbred lines. Three haplotypes, named SbPH11-Hap1, SbPH11-Hap2 and SbPH11-Hap3, at the SbPH11 locus with different combination of the two SNPs have been identified. The plant height of SbPH11-Hap2 materials was extremely significantly higher than that of SbPH11-Hap1 and SbPH11-Hap3 materials, and that of SbPH11-Hap1 materials was extremely significantly higher than that of SbPH11-Hap3 materials. The KASP marker that associates to plant height was developed based on two functional SNPs of the gene SbPH11. The marker was used to genotype and phenotype of 30 sorghum germplasms resources. The KASP marker was confirmed to accurately identify two functional SNPs of SbPH11. These results suggested that the newly-developed KASP marker can efficiently and accurately predict the plant height in sorghum germplasm resources, and can be applied in early screening and molecular marker-assisted selection in breeding for sorghum with higher plant height.

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引用本文

段国旗,吕娜,石颖怡,等.高粱株高相关基因SbPH11分子标记的开发和应用[J].植物遗传资源学报,2024,25(1):111-119.

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  • 收稿日期:2023-06-11
  • 最后修改日期:2023-07-24
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  • 在线发布日期: 2024-01-08
  • 出版日期: 2024-01-08
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