小麦农家品种武都白茧儿抗条锈病基因遗传定位
作者:
作者单位:

1.新疆农业大学农学院,乌鲁木齐 830052;2.中国农业科学院作物科学研究所,北京 100081;3.宁夏农林科学院 农作物研究所,银川 750002;4.甘肃农业科学院小麦研究所,兰州 730030

作者简介:

研究方向为作物遗传育种,E-mail:1669626641@qq.com

通讯作者:

耿洪伟,研究方向为作物遗传育种,E-mail:hw-geng@163.com
刘金栋,研究方向为作物遗传育种,E-mail:liujindong@caas.cn

中图分类号:

基金项目:

中国科学技术协会青年人才托举工程计划(2020QNRC001);国家自然科学基金(32272186);中国农业科学院南繁研究专项(YBXM2303)


Genetic Mapping of Stripe Rust Resistance Gene/Locus in Landrace Wheat Wudubaijianer
Author:
Affiliation:

1.College of Agronomy,Xinjiang Agricultural University,Urumqi 830052;2.Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081;3.Crop Research Institute, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan 750002;4.Wheat Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730030

Fund Project:

Foundation projects: The Young Elite Scientists Sponsorship Program by China Association for Science and Technology (2020QNRC001); National Natural Science Foundation of China (32272186);Nanfan Special Project, Chinese Academy of Agricultural Sciences (YBXM2303)

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    摘要:

    小麦条锈病是由条形柄锈菌(Pst, Puccinia striiformis f. sp. tritici)引起的一种全球性重要真菌病害,严重影响小麦生产安全。发掘抗病基因,培育抗病品种是防治条锈病最为经济有效和安全的方法。武都白茧儿是甘肃陇南小麦农家品种,在整个生育期都表现出中到高抗条锈病,但其抗性基因尚不明晰。为解析武都白茧儿的条锈病抗性遗传机制,本研究利用抗病亲本武都白茧儿和感病亲本辉县红配制杂交组合,利用条锈菌生理小种V31/lab对其F2和F2:3群体进行苗期接种鉴定,结合混池测序和连锁分析定位抗性基因。结果表明,武都白茧儿对V31/lab抗性由一对隐性基因控制,暂命名为yrWUD。根据F2群体的混池外显子捕获测序和混池转录组测序结果,开发了12个高通量的KASP标记;通过连锁分析,将yrWUD定位在4AL染色体的2.6 cM遗传区间内,与侧翼标记4AL364AL11的遗传距离分别为0.9 cM和1.7 cM,对应13 Mb的物理区间(4A:610.26~623.35 Mb),其中3个抗病相关基因TraesCS4A02G329100TraesCS4A02G330000TraesCS4A02G330100在抗感池中差异表达,推测为yrWUD的候选基因。本研究定位了武都白茧儿的抗性基因yrWUD,用KASP标记4AL36在自然群体中检测了该基因的等位基因,研究结果为小麦抗条锈病育种提供了新基因和分子标记。

    Abstract:

    Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a global devastating fungal disease that severely affects wheat production. Exploring resistance genes and their use in breeding are the most economical and effective method for controlling stripe rust. Wudubaijianer, a wheat landrace from Longnan region, Gansu province, China, showed moderate to high resistance to stripe rust in the lifecycle, but the resistance basis remains unclear. To decipher its genetic mechanism of stripe rust resistance, the F2 and F2:3 populations derived from a cross between Wudubaijianer and a susceptible parent Huixianhong were inoculated with the Pst race V31/lab at the seedling stage, and were sampled for BSE-Seq (Bulked segregant exome capture sequencing), BSR-Seq (Bulked segregant RNA-Seq) and linkage analysis. The results indicated that resistance to V31/lab in Wudubaijianer was conferred by a recessive locus, tentatively designated yrWUD. Based on BSE-Seq and BSR-Seq, 12 KASP (Kompetitive allele-specific PCR) markers were developed. The linkage analysis mapped yrWUD to a 2.6 cM genetic interval on chromosome 4AL, flanked by markers 4AL36 and 4AL11 with genetic distance of 0.9 and 1.7 cM, respectively, corresponding to a physical interval of 13 Mb (4A:610.26-623.35Mb) harboring three disease-resistance related genes, TraesCS4A02G329100TraesCS4A02G330000 and TraesCS4A02G330100. These three genes were detected with differential expression between the resistant and susceptible pools, and were proposed as candidate genes for yrWUD. Additionally, the KASP marker 4AL36 was validated in a natural population. Collectively, this study identified a stripe rust resistance locus yrWUD in Wudubaijianer and validated its effect in a natural population, providing a new resistance gene and molecular markers for selecting stripe rust resistance in wheat breeding.

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引用本文

董亚超,董艳,赵聪,等.小麦农家品种武都白茧儿抗条锈病基因遗传定位[J].植物遗传资源学报,2024,25(10):1737-1747.

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  • 收稿日期:2024-01-02
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  • 在线发布日期: 2024-10-09
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