马铃薯基因StAVP1提高拟南芥抗旱性的功能鉴定
作者:
作者单位:

1.宁夏农林科学院农业生物技术研究中心,银川750002;2.宁夏农业生物技术重点实验室,银川750002;3.宁夏农业技术推广总站,银川750002;4.宁夏农林科学院固原分院,固原756009

作者简介:

研究方向为植物分子生物育种,E-mail:429625104@qq.com

通讯作者:

巩檑,研究方向为植物分子生物育种,E-mail:tea_gl@126.com

中图分类号:

基金项目:

宁夏自然科学基金(2022AAC03431,2021AAC05015,2022AAC03429);国家自然科学基金(32260504);宁夏回族自治区农业科技自主创新专项科技创新引导项目(NKYG-22-02);宁夏回族自治区农业育种专项(2019NYYZ01)


Functional Identification of Potato Gene StAVP1 to Enhance Drought Resistance of Arabidopsis thaliana
Author:
Affiliation:

1.Agricultural Biotechnology Research Center, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002;2.Ningxia Key Laboratory of Agricultural Biotechnology, Yinchuan 750002;3.Ningxia Agricultural Technology Extension Station, Yinchuan 750002;4.Guyuan Branch, Ningxia Academy of Agriculture and Forestry Sciences, Guyuan 756009

Fund Project:

Foundation projects: Natural Science Foundation Project of Ningxia(2022AAC03431, 2021AAC05015, 2022AAC03429);National Natural Science Foundation of China(32260504);Agricultural Science and Technology Independent Innovation Special Scientific and Technological Innovation Guidance Project of Ningxia(NKYG-22-02);Agricultural Breeding Project of Ningxia(2019NYYZ01)

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    摘要:

    马铃薯(Solanum tuberosum L.)是全球第四大粮食作物,挖掘鉴定抗旱基因,是培育抗旱马铃薯新品种的重要途径。马铃薯StAVP1基因参与跨液泡膜物质运输,在植物应对物质运输和非生物胁迫中发挥重要作用。本研究从马铃薯品种大西洋的叶片中克隆得到编码区全长2301 bp的StAVP1基因,生物信息学分析表明,其包含典型的H+-焦磷酸酶结构域和13个跨膜结构域,与近缘物种的AVP1蛋白具有较高的序列相似性,预测定位于液泡膜上。组织表达模式分析显示,StAVP1基因在花中表达量最高,块茎中表达量最低。StAVP1过表达拟南芥株系的生物量和根长显著高于野生型,干旱胁迫后,过表达StAVP1拟南芥株系叶丙二醛含量显著低于野生型,超氧化物歧化酶、过氧化物酶和脯氨酸含量显著高于野生型。DREB等逆境应答基因表达量显著上调。CoIP试验结果显示,StAVP1与StRAB发生直接互作,且干旱胁迫下StAVP1过表达拟南芥株系中AtRAB基因表达量也显著上调。本研究通过生物信息分析、异源表达表型、生理、基因表达方面功能验证、互作蛋白分析,表明马铃薯StAVP1基因是马铃薯遗传改良的重要基因来源。

    Abstract:

    Potato (Solanum tuberosum L.) is the fourth largest food crop in the world. To explore and identify the biological basis of drought resistance genes is an important way to cultivate new varieties of drought-resistant potato. Potato StAVP1 gene is involved in material transport across the vacuolar membrane and plays an important role in plant response to material transport and abiotic stress. In this study, the StAVP1 gene with the full length of 2301 bp coding region was cloned from the leaves of ‘Atlantic’ potato. Bioinformatics analysis showed that the StAVP1 gene contained typical H+-pyrophosphatase domain and 13 transmembrane domains, which had high sequence similarity with AVP1 protein of related species, and was predicted to be located on the vesicular membrane. Tissue expression pattern analysis showed that StAVP1 gene expression was the highest in flowers and the lowest in tubers. The biomass and root length of Arabidopsis thaliana plants overexpressed in StAVP1 were significantly higher than those of wild type, and the contents of malondialdehyde in leaves of Arabidopsis thaliana plants overexpressed in StAVP1 were significantly lower than those of wild type under drought stress, while the contents of superoxide dismutase, peroxidase and proline were significantly higher than those of wild type. Stress response genes such as DREB were significantly up-regulated. The results of CoIP test showed that StAVP1 directly interacted with StRAB, and AtRAB gene expression was significantly up-regulated in Arabidopsis thaliana strains overexpressed in StAVP1 under drought stress. In this study, through biological information analysis, heterologous expression phenotype, physiological, functional verification of gene expression, protein interaction analysis, potato StAVP1 gene is an important gene source for potato genetic improvement.

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引用本文

杨文静,刘璇,崔勇,等.马铃薯基因StAVP1提高拟南芥抗旱性的功能鉴定[J].植物遗传资源学报,2024,25(10):1793-1802.

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  • 收稿日期:2024-01-02
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  • 在线发布日期: 2024-10-09
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