辣椒地方品种和创新种质的遗传比较分析
作者:
作者单位:

江汉大学生命科学学院/湖北省豆类(蔬菜)植物工程技术研究中心/湖北省食用豆类自然科技资源中心,武汉 430056

作者简介:

研究方向为植物资源与遗传育种,E-mail:1328832809@qq.com

通讯作者:

陈禅友,研究方向为植物资源与遗传育种,E-mail:ccy@jhun.edu.cn

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基金项目:

湖北省技术创新专项(重大项目)(2017 ABA147);湖北省教育厅百校联百县项目(BXLBX0360,BXLBX0361)


Genetic Comparative Studies on Innovated Germplasm and Local Varieties of Chili Pepper
Author:
Affiliation:

School of Life Sciences,Jianghan University/Hubei Province Engineering Research Center for Legume Plants/ Hubei Province Natural Science Resource Center of Edible Legume, Wuhan 430056

Fund Project:

Foundation projects: Hubei Province Technology Innovation Project (Major Program) (2017ABA147);Hubei Provincial Department of Education's Hundred Schools and Hundred Counties Project(BXLBX0360,BXLBX0361)

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    摘要:

    观测了61份辣椒地方品种以及39份紫彩椒和红辣椒809的杂交后代选育的创新种质的12个性状以及基因组DNA的SSR分子标记信息,进行了遗传相关分析和多样性评价。结果表明:地方品种和创新种质两个群体间的12个性状均呈显著差异,其性状变异系数范围分别为1.15%~50.02%和3.27%~60.67%,其中地方品种的维生素C含量变异系数最大;两个群体的广义遗传力分别在79.24%~99.23%和87.73%~98.80%之间,说明性状主要由遗传决定。两群体间的性状分化系数在1.30%~48.95%之间,其中花色苷含量分化明显,株高分化不明显。相关性分析表明两两性状间有23组呈显著或极显著相关;主成分分析综合评价筛选出10份特异资源。筛选出20对SSR有效引物对,PCR扩增出68个多态性条带,多态性比例为69.39%,其中地方品种的多态性位点居多。基于12个性状和分子标记数据进行聚类分析,可将供试种质划分为3个类群,地方品种大部分聚集在类群Ⅰ,创新种质大部分聚集在类群Ⅱ,类群Ⅲ仅含PP1122种质,该种质植株具有叶片大、长势旺、果实大、果皮紫色且营养成分含量高等特点,说明创新种质与地方品种亲缘关系较远,利用不同类群的种质紫彩椒和红辣椒809的杂交可拓展辣椒种质的遗传背景。本研究为辣椒种质创新和品种遗传改良提供参考。

    Abstract:

    Through observing twelve phenotypic traits and SSR genotyping result, this study analyzed 61 local pepper varieties and 39 offspring lines from the hybrids of 'Purple Color Pepper' and 'Red Pepper 809'. The results showed that twelve traits in the two populations of local pepper varieties and innovated pepper germplasm were significantly different (P≤0.05), with the variation coefficient of the traits ranged from 1.15%-50.02% and 3.27%-60.67%, respectively, of which in local varieties vitamin C content was detected with the highest variations. The broad-sense heritabilities arranged from 79.24%-99.23% and 87.73%-98.80%, respectively, indicating that these traits were highly inheritable. Traits differentiation coefficients between the two populations arranged from 1.30% to 48.95%, of which anthocyanin content was the trait with the most obvious differentiation, while plant height had no obvious differentiation. Correlation analysis revealed 23 pairs of traits that were significantly or extremely significantly correlated. Comprehensive evaluation through principal component analysis identified 10 specific resources. In addition, 20 SSR markers identified 68 polymorphic bands, with the polymorphism ratio of 69.39%, and the higher polymorphic loci were detected in local varieties. Based on the clustering analysis of the twelve traits and molecular markers information, these germplasms were assigned to three groups, of which group I included most local varieties, and group II included most innovated germplasms, and group III contained 'PP1122' germplasm only. ‘PP1122’ showed large leaves, vigorous fruit, purple peel and high nutrient content. The hybridization of varieties between different groups, such as 'Purple colored pepper' and 'Red pepper 809', was able to enlarge the genetic basis of pepper germplasm, providing a reference for pepper germplasm innovation and variety genetic improvements.

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孟俊瑛,郭瑞,陈高,等.辣椒地方品种和创新种质的遗传比较分析[J].植物遗传资源学报,2024,25(10):1685-1696.

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  • 收稿日期:2024-01-08
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  • 在线发布日期: 2024-10-09
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