干旱胁迫及复水处理对白菜型油菜生理指标和DNA甲基化水平的影响
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1.甘肃农业大学农学院;2.省部共建干旱生境作物学国家重点实验室;3.甘肃天水市农业科学研究所

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甘肃省高校产业支撑计划项目(2023CYZC-51);财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-12);国家重点研发计划专项子课题(2022YFD1100500)


Effects of drought stress and rewatering treatments on physiological indicators and DNA methylation levels in B.rapa
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Gansu Province University Industry Support Plan Project ( 2023CYZC-51 ) ; ministry of Finance and Ministry of Agriculture and Rural Affairs National Modern Agricultural Industry Technology System Construction Project ( CARS-12 ) ; special Sub-project of National Key R & D Program ( 2022YFD1100500 )

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    摘要:

    为了解析干旱处理对白菜型冬油菜生理指标及DNA甲基化水平的影响,本研究对白菜型油菜进行正常供水、干旱胁迫、干旱后复水3个处理,分析不同处理后油菜生理指标及DNA甲基化水平的变化。结果表明,当白菜型油菜受到干旱胁迫时,丙二醛含量、抗氧化酶活性及渗透调节物质含量均有所增加,增加幅度分别为167.27%、254.85%、103.24%、164.64%、49.2%、37.79%,渗透调节物质增加量分别为:3.21μg·g-1FW、0.048μg·mg-1FW,叶片相对含水量显著下降,下降幅度为53.64%。复水后各项生理指标得到显著恢复;干旱后油菜甲基化水平呈上升趋势,复水后甲基化率高于干旱和对照,甲基化率达到20.56%。GO富集结果显示,差异甲基化基因在生物学过程、结合及分子功能富集。KEGG功能富集分析表明,差异甲基化基因主要在泛素介导的蛋白水解、胞吞作用、ABC转运蛋白、光合生物中的碳固定等代谢相关通路中富集。甲基化率和生理指标之间存在一定的相关性,结合GO和KEGG的富集结果,7个差异甲基化基因编码过氧化物酶。

    Abstract:

    To analyze the effects of drought treatments on physiological indexes and DNA methylation levels of winter Brassica Rapa, this study subjected cabbage-type oilseed rape to three treatments, namely, normal water supply, drought stress, and rehydration after drought, and analyzed the changes in the physiological indexes of oilseed rape and the DNA methylation levels of oilseed rape after the different treatments. The results showed that when cabbage-type oilseed rape was subjected to drought stress, malondialdehyde content, antioxidant enzyme activity and osmoregulatory substances increased by 167.27%、254.85%、103.24%、164.64%、49.2% and 37.79%, respectively, and osmoregulatory substances increased by 3.21μg·g-1FW, 0.048 μ g·mg-1FW, and the relative water content of leaves decreased significantly by 53.64%. The physiological indexes were significantly recovered after rewatering; the methylation level of oilseed rape showed an increasing trend after drought, and the methylation rate after rewatering was higher than that of drought and control, with the methylation rate reaching 20.56%.The GO enrichment results showed that the differentially methylated genes were enriched in the biological process, binding and molecular function.KEGG functional enrichment analysis showed that the differentially methylated genes were mainly involved in the ubiquitin mediated protein hydrolysis, cytotoxicity , ABC transporter proteins, carbon fixation in photosynthetic organisms, and other metabolism-related pathways. There was a correlation between methylation rate and physiological indicators, and combined with the GO and KEGG enrichment results, seven differentially methylated genes encoded peroxidases.

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  • 收稿日期:2024-01-12
  • 最后修改日期:2024-06-28
  • 录用日期:2024-08-30
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