基于表型性状和品质性状的苦荞核心种质构建
作者:
作者单位:

1.山西农业大学农业基因资源研究中心/农业农村部黄土高原作物基因资源与种质创制重点实验室/杂粮种质资源发掘与 遗传改良山西省重点实验室, 太原 030031;2.山西农业大学农学院,太谷 030801

作者简介:

研究方向为荞麦燕麦种质资源创新利用,E-mail:mamingchuan6@163.com

通讯作者:

刘龙龙,研究方向为燕麦荞麦种质资源创新利用, E-mail:lllong781211@sina.com

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基金项目:

山西省重点研发计划项目(2022ZDYF110);山西省高等学校科技创新项目(2022L092);山西农业大学杂粮研究院项目(ZL20220301);山西省科技重大专项计划“揭榜挂帅”项目(202101140601027);国家现代农业产业技术体系(CARS-07-A-2);中央引导地方科技发展资金项目(YDZJSX2021A031)


Construction of Core Collection of Tartary Buckwheat Based on Phenotypic and Quality Traits
Author:
Affiliation:

1.Center for Agricultural Genetic Resources Research,Shanxi Agricultural University/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture and Rural Affairs/Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan 030031;2.College of Agriculture, Shanxi Agricultural University, Taigu 030801

Fund Project:

Foundation projects: Key R&D Project in Shanxi Province(2022ZDYF110); The Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(2022L092); Research Project of Minor Crops Research Institute in Shanxi Agricultural University(ZL20220301);The Major Special Science and Technology Plan “Unveiling and Commanding” Projects in Shanxi Province (202101140601027);China Agriculture Research System of MOF and MARA(CARS-07-A-2);The Central Government Guides Local Science and Technology Development Fund Projects(YDZJSX2021A031)

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    摘要:

    利用708份国内外苦荞资源的22个表型性状和3个品质性状数据,进行苦荞核心种质构建。通过比较遗传距离、取样方法、取样比例和聚类方法4个层面的不同组合策略优劣,确定了苦荞核心种质的最佳取样策略为“欧氏距离+多次聚类偏离度取样法+20%取样比例+最长距离法”,构建了包含141份苦荞资源的核心种质。核心种质和原始种质的均值差异百分率、方差差异百分率、极差符合率和变异系数变化率分别为 0、84.00%、97.60%和115.42%。比较核心种质与原始种质各性状的均值、方差、极值、变异系数、多样性指数和主成分对核心种质进行验证和评价。核心种质与原始种质在25个性状上的均值无显著差异;核心种质在所有性状上的方差均大于或等于原始种质,其中22个性状的方差差异达到显著或极显著水平,表明核心种质有更强的异质性;多样性指数t检验表明,核心种质与原始种质多样性指数在所有性状上差异均不显著;主成分检验表明,核心种质与原始种质均有8个主成分,累计贡献率分别为77.525%和76.191%。本研究所构建的核心种质能较好地代表原始种质,可作为苦荞种质资源收集保存和有效利用的依据。

    Abstract:

    In this study, the data of 22 phenotypic traits and 3 quality traits of 708 tartary buckwheat resources were used to construct the core collection of tartary buckwheat germplasm. By comparing merits of four different combination strategies including the genetic distance, sampling method, sampling proportion and clustering method, the ‘euclidean distance + multiple cluster deviation sampling + 20% sampling proportion + maximum distance method’ was determined as the optimal sampling strategy of tartary buckwheat core collection and the core collection containing 141 tartary buckwheat resources was constructed. The mean difference percentage, variance difference percentage, range coincidence rate and variation coefficient change rate of core collection and original collection were 0,84.00%, 97.60% and 115.42%, respectively. Finally, the phenotypic and quality traits of the core collection were compared with the original germplasm. The mean value of 25 traits between core collection and original germplasm had no significant difference. The variance of core collection of 25 traits was higher than or equal to that of original germplasm, and the variance of 22 traits between core collection and original germplasm had significant difference at 0.05 leve or 0.01 level. It showed that the heterogeneity of core collection was better. t-test of Shannon-Weaver index showed that there was no significant difference between core collection and original germplasm on all traits. Both the core collection and the original germplasm had 8 principal components, and the cumulative contribution rates were 77.525% and 76.191%,respectively. The core collection germplasm was proved to represent the genetic diversity of the original germplasm, which might be useful in collection, preservation and effective utilization of tartary buckwheat germplasm resources.

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引用本文

马名川,赵少迪,胡传伟,等.基于表型性状和品质性状的苦荞核心种质构建[J].植物遗传资源学报,2024,25(10):1637-1647.

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  • 收稿日期:2024-01-31
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  • 在线发布日期: 2024-10-09
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