基于SSR标记与农艺性状对三七集团选择群体的评价
作者:
作者单位:

1.云南农业大学西南中药材种质创新与利用国家地方联合工程研究中心,昆明 650201;2.云南农业大学云南省药用植物生物学重点实验室,昆明 650201;3.云南农业大学农学与生物技术学院,昆明 650201;4.云南省农业科学院经济作物研究所,昆明 650225;5.文山学院三七研究院,云南文山 663099;6.文山苗乡三七科技有限公司,云南文山 663000

作者简介:

研究方向为三七种质资源评价与鉴定,E-mail:13988543978@163.com

通讯作者:

刘冠泽,研究方向为药用植物遗传育种,E-mail: guanzeliu@163.com

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基金项目:

国家自然科学基金(32260095);云南省重大科技专项(202102AA100052-02,202102AA310048-01);云南省科技人才与平台建设(202305AR340004)


Evaluation of Mass Selected Populations of Panax notoginseng Based on SSR Markers and Agronomic Traits
Author:
Affiliation:

1.National Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming 650201;2.Yunnan Provincial Key Laboratory of Medicinal Plant Biology, Yunnan Agricultural University, Kunming 650201;3.College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201;4.Institute of Cash Crops, Yunnan Academy of Agricultural Sciences, Kunming 650225;5.Notoginseng Research Institute, Wenshan University, Wenshan 663099,Yunnan;6.Miaoxiang Panax Notoginseng Science and Technology Corporation Ltd., Wenshan 663000,Yunnan

Fund Project:

Foundation projects: National Natural Science Foundation of China(32260095); Major Science and Technology Projects of Yunnan Province(202102AA100052-02, 202102AA310048-01);Yunnan Province Science and Technology Talent and Platform Development(202305AR340004)

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    摘要:

    三七为人参属多年生草本植物,生长和繁育周期长,群体间的遗传多样性低,但群体内单株间表型变异大,人参皂苷含量差异尤为明显。本研究根据三七中5种人参皂苷(三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1、人参皂苷Re、人参皂苷Rd)含量构建了11份集团群体(SL1~SL11),对当代和第一代集团群体人参皂苷含量进行了比较分析。同时,基于三七基因组,使用MISA软件进行全基因组水平SSR标记开发,鉴定到255239个SSR标记,筛选出17对多态性SSR标记。使用SSR标记对第一代群体进行遗传多样性评价。结果表明,11份第一代集团群体观察杂合度Ho较高(0.4583~0.6042),遗传分化程度低(Fst=0.0447),且具有较高基因流(Nm=11.6189);第一代群体间总皂苷含量无显著性差异而三七皂苷R1变异系数高于总皂苷和其他单体皂苷,且群体SL8三七皂苷R1含量显著高于其他群体。综上,本研究以皂苷含量为目标性状构建了高皂苷含量的集团群体,并利用SSR标记评价了群体遗传多样性,构建的三七皂苷R1高含量群体可作为后续育种材料。

    Abstract:

    Panax notoginseng is a perennial herb with long growth period and reproduction cycle. The inter-population diversity was low while the intra-population diversity among individual plants remained higher. Among them, the ginsenosides content of individual plants were significantly different among groups, providing a theoretical basis for deploying the group selection approach. In this study, 11 group populations (SL1-SL11) were constructed according to the content of five saponins (notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1, ginsenoside Re, ginsenoside Rd) in P. notoginseng. The contemporary and first generation group population were compared and analyzed base on the saponins content. In addition, the genetic diversity of populations was evaluated by using 17 pairs of polymorphic SSR markers that were identified from a total of 255239 SSR polymorphisms. Resulted from the genetic diversity of the first generation population of SL1-SL11 that were evaluated by SSR markers, the high heterozygosity (0.4583-0.6042), low genetic differentiation (Fst=0.0447), and high gene flow (Nm=11.6189) were observed. Although there were no significant difference in total saponin among the populations, the coefficient of variation for notoginsenoside R1 was higher than that for total content of five saponins and other monomeric saponins. The notoginsenoside R1 content in the SL8 population was significantly higher than that of other populations. Collectively, this study produced group populations using saponin content as the target trait, and evaluated their genetic diversity using SSR markers. The newly-generated population with higher notoginsenoside R1 content can serve as breeding material in breeding of Panax notoginseng.

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引用本文

李满桥,王前,李葵秀,等.基于SSR标记与农艺性状对三七集团选择群体的评价[J].植物遗传资源学报,2024,25(10):1673-1684.

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  • 收稿日期:2024-01-31
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  • 在线发布日期: 2024-10-09
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