Landrace are important germplasm resources for wheat breeding. In order to uncover the distribution of dwarfing genes in landraces， we detected the allelic variation types of dwarfing genes and their distribution frequencies in different wheat regions of Gansu province. The results showed that： （1） the frequency of Rht-B1b and Rht-D1b in landraces was extremely low. 41.4% of landraces carried Rht8， with higher frequency in spring wheat regions than in winter wheat regions. 46.7% of landraces contained Rht24b， with lower frequency in spring wheat regions than in winter wheat regions. The frequency of Ppd-D1a was only 17.8%， with lower frequency in spring wheat regions than in winter wheat regions. In addition， only three combinations （Rht-D1b/Rht8， Rht-D1b/Rht24b， and Rht8/Rht24b） were detected， with frequencies of 0.2%， 0.5%， and 12.8%， respectively. （2） The frequency and combination distribution of dwarfing genes carried by landraces were lower than those of cultivars， and the differences were significant. Different sources of cultivars carried the different dominant dwarfing allelic variations and frequencies. Cultivars Qingshui test station mainly carried Rht-D1b， Rht8， and Rht24b， cultivars from Huangyang test station mainly carried Rht-B1b， Rht-D1b， Rht8， and Rht24b， and cultivars from Gangu test station mainly carried Rht8 and Rht24b.Cultivars from Qingshui and Huangyang test station had dwarf stature and high yield potential， suitable for wheat breeding in Hexi and Yellow River irrigation areas，Longnan and Longdong. Cultivars from Gangu test station had higher plant height and better disease resistance， suitable for disease improvement in dryland wheat in Dingxi， Tianshui， Longnan， and Longdong， etc. （3） Based on the molecular marker results， 15 landraces and 31 cultivars were selected with two or more dwarfing genes （including dwarfing genes or Ppd-D1a）， which can provide parental materials for wheat breeding in different wheat areas of Gansu.