葡萄种质资源果实形状的鉴定评价
DOI:
作者:
作者单位:

中国农业科学院郑州果树研究所

作者简介:

通讯作者:

中图分类号:

S663.1

基金项目:

河南省科技攻关(232102110206);中国农业科学院科技创新工程专项经费项目(CAAS-ASTIP-2023-ZFRI); 现代农业产业技术体系建设专项资金(CARS-29-yz-1)


Identification and Evaluation of Berry Shape in Grape Germplasm Resources
Author:
Affiliation:

Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences

Fund Project:

The scientifc and technological research in Henan Province (232102110206); The Agricultural Science and Technology Innovation Program (CAAS-ASTIP-2023-ZFRI); The China Agriculture Research System (CARS-29-yz-1)

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    对葡萄种质资源果形性状进行鉴定评价,探究不同果形葡萄的种质特点和性状差异,验证果形相关基因的表达模式。以国家葡萄种质资源圃(郑州)的666份葡萄种质资源为实验材料,对葡萄果实的形状、纵横径、单粒重和种子粒数等进行统计分析和综合评价,并对果实形状相关基因的表达量进行验证。葡萄栽培品种果形类型丰富,可分为圆形、扁圆形、长圆形、近圆形、长椭圆形、椭圆形、倒卵形、卵圆形、弯形、鸡心形和束腰形11种,野生种质果实基本为圆形,仅刺葡萄中有少量椭圆形和鸡心形种质。葡萄果形一般可以通过外观识别,个别如圆形和近圆形需要参考纵横径进行描述和确定。欧亚种较欧美杂种果形类型更丰富;酿酒葡萄主要为圆形和椭圆形,较鲜食葡萄果形类型少;圆形和鸡心形葡萄平均单粒重较小,长圆形和倒卵形葡萄平均单粒重较大;葡萄单粒重与纵、横径存在正相关关系,不同果形的回归方程存在一定差异;葡萄种子数量以2-3粒居多,其中,具有5-6粒种子的种质多为圆形果,无核品种果实以椭圆形居多。对葡萄果形候选基因进行实时荧光定量分析,基因VvFUL1A,VvSUN在长果与圆果中的表达量有显著差异,可能是调控葡萄果形的重要候选基因。本研究通过对666份葡萄种质的果实性状进行系统鉴定评价,探究了不同果形葡萄的种质特点,初步了解了不同果形葡萄的性状差异,为将来葡萄育种工作以及围绕果形开展的研究工作提供了一定参考。

    Abstract:

    The objective of this study was to identify and evaluate the berry shape traits of grape germplasm resources, investigate the variations in varieties characteristics and traits among different grape cultivars with distinct berry shapes, and validate the expression patterns of genes associated with berry shape. This study systematically investigated on the berry traits of 666 germplasm resources of grapes, and statistically analyzed the vertical and horizontal diameter, single berry weight and number of seed. According to the standards and description methods of the Description and Data Standard for Grape (Vitis) , the berry shapes of cultivated varieties were categorized into 11 types: round,obloid,oval shape,nearly round,long oval,broad lipsoid,obovoid,ovoid,curved shape,chicken heart shape and waist shape. The cultivated varieties were mainly round and broad lipsoid, followed by nearly round. The berry shape of wild varieties were basically round, only 4 broad lipsoid and 1 chicken heart shape. Grape berry shape could generally be identified by appearance, but some berry shape, such as round and near round, should be described and determined by referring to the vertical and horizontal diameter. Vitis vinifera L. and V. vinifera × V. labrusca dominated the grape varieties, accounting for more than 88% of the varieties, and the number of V. vinifera L. varieties weremore than twice that of V. vinifera × V. labrusca Grapes has a wide range of uses, with fresh consumption and winemaking being the primary ones. Wine grapes had fewer berry shape types, except round and broad lipsoid, with only a small quantity of nearly round, long oval, ovoid and chicken heart shape varieties. Statistical analysis of grape individual berry weight across different berry shapes revealed that the average individual berry weight of round and chicken heart grape was less, and that of oval shape and obovoid grape was heavier. The regression analysis of grape vertical and transverse diameters against individual berry weight showed that the regression equations for most grape shapes had good fit. All regression equations were positive coefficients, indicating that the individual berry weight of different grape shapes was positively correlated with the vertical and transverse diameters of grapes. The number of seeds ranged from 0 to 6. Among these, grape varieties with 2 seeds are the most common, followed by 3 seeds. Varieties with 5 and 6 seeds were the least common and were primarily found in round grapes. qRT-PCR analysis of grape berry shape candidate genes was performed, the genes VvFUL1A and VvSUN might be important candidate genes regulating grape berry shape This study explored the varieties characteristics of different fruit shapes and preliminarily understood the character differences of different berry shapes, which provide a certain reference for berry shape and future grape breeding research.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2024-02-22
  • 最后修改日期:2024-04-03
  • 录用日期:2024-08-19
  • 在线发布日期:
  • 出版日期:
您是第位访问者
ICP:京ICP备09069690号-23
京ICP备09069690号-23
植物遗传资源学报 ® 2024 版权所有
技术支持:北京勤云科技发展有限公司