Self-incompatibility of Cruciferous crop is primarily controlled by the Multiple alleles of S locus. Ornamental kale（Brassica oleracea var. acephala）belongs to Brassica family, and is a typical self-incompatibility subspecies. In this study, the fingerprint map of 20 ornamental kale inbred lines was first constructed using 20 pairs of SSR primers. A total of 65 allelic variations were identified by this set of primers，with an average of 3.25 variations per pair of primers. The unweighted pair group method with arithmetic mean（UPGMA） clustered them into three genetic groups：OK1，OK2，and OK3. A total of nine SRK haplotypes were identified in 20 ornamental kale inbred lines using specific primers in the kinase region of SRK gene, including eight class I S haplotypes （SRK7，SRK13，SRK14，SRK16，SRK23，SRK36，SRK45，and SRK51）and one class II S haplotype （SRK15）. In the genetic group OK1，the major haplotype is SRK15 haplotype，accounting for 50%，followed by SRK23 haplotype，accounting for 20%. In the genetic group OK2，the major haplotype is SRK15 and SRK16 haplotype，accounting for 40%，respectively. In the genetic group OK3，the major haplotype is SRK23，accounting for 40%. OK1 genetic group has a relatively higher ratio of SRK15 haplotypes. The determination of the self-incompatible index further verified the compatibility among inbred lines from the same or different genetic groups，emphasizing that the hybrid development should consider both the genetic groups and SRK haplotype.