羽衣甘蓝SSR指纹图谱构建及不同遗传类群的SRK单元型分布
DOI:
作者:
作者单位:

1.青岛农业大学园艺学院;2.中国农业科学院蔬菜花卉研究所

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学(32172578)


Construction of SSR Fingerprint and SRK Haplotype Distribution of Different Genetic Groups in Ornamental Kale(Brassica oleracea var. acephala)
Author:
Affiliation:

1.Qingdao Agricultural University;2.Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences

Fund Project:

the National Natural Science Foundation of China [32172578]

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    十字花科作物主要受S位点的复等位基因控制着自交不亲和性(SI,self-incompatibility),羽衣甘蓝(Brassica oleracea var. acephala)是十字花科芸薹属中典型的自交不亲和性植物。本研究利用20对SSR引物首次构建了20个羽衣甘蓝自交系的指纹图谱,这套引物共鉴定到65个等位变异,平均每对引物含有3.25个变异。等权重算术平均聚类法(UPGMA)将它们聚类为3个遗传类群:OK1、OK2和OK3。利用SRK基因激酶区的特异性引物分析发现20个羽衣甘蓝自交系中共有9个SRK单元型,分别是8个Ⅰ类S单元型(SRK7、SRK13、SRK14、SRK16、SRK23、SRK36、SRK45和SRK51)和1个Ⅱ类S单元型(SRK15)。遗传类群OK1主要为SRK15单元型,占比50%,其次为SRK23单元型,占比20%;遗传类群OK2主要为SRK15、SRK16单元型,占比均为40%;遗传类群OK3主要为SRK23单元型,占比40%,OK1遗传类群拥有较多的SRK15单元型。亲和指数测定结果进一步验证了不同遗传类群内部或遗传类群间的自交系杂交的亲和关系,杂交组合配制的过程中应兼顾遗传类群和SRK单元型。

    Abstract:

    Self-incompatibility of Cruciferous crop is primarily controlled by the Multiple alleles of S locus. Ornamental kale(Brassica oleracea var. acephala)belongs to Brassica family, and is a typical self-incompatibility subspecies. In this study, the fingerprint map of 20 ornamental kale inbred lines was first constructed using 20 pairs of SSR primers. A total of 65 allelic variations were identified by this set of primers,with an average of 3.25 variations per pair of primers. The unweighted pair group method with arithmetic mean(UPGMA) clustered them into three genetic groups:OK1,OK2,and OK3. A total of nine SRK haplotypes were identified in 20 ornamental kale inbred lines using specific primers in the kinase region of SRK gene, including eight class I S haplotypes (SRK7,SRK13,SRK14,SRK16,SRK23,SRK36,SRK45,and SRK51)and one class II S haplotype (SRK15). In the genetic group OK1,the major haplotype is SRK15 haplotype,accounting for 50%,followed by SRK23 haplotype,accounting for 20%. In the genetic group OK2,the major haplotype is SRK15 and SRK16 haplotype,accounting for 40%,respectively. In the genetic group OK3,the major haplotype is SRK23,accounting for 40%. OK1 genetic group has a relatively higher ratio of SRK15 haplotypes. The determination of the self-incompatible index further verified the compatibility among inbred lines from the same or different genetic groups,emphasizing that the hybrid development should consider both the genetic groups and SRK haplotype.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2024-02-26
  • 最后修改日期:2024-04-07
  • 录用日期:2024-08-19
  • 在线发布日期:
  • 出版日期:
您是第位访问者
ICP:京ICP备09069690号-23
京ICP备09069690号-23
植物遗传资源学报 ® 2024 版权所有
技术支持:北京勤云科技发展有限公司