The rice variety “Shaoxiang100” with low cadmium accumulation was developed through heavy-ion mutagenesis of the aromatic high-quality rice material 44-5, combined with the M1TDS technology, and the mutation of the OsNRAMP5 gene. The variety “Shaoxiang100” underwent testing and trials in multiple locations throughout Hunan Province from 2021 to 2023. It also partici-pated in national independent trials for varieties with low Cd accumulation. The mutation of the OsNRAMP5 gene reduces man-ganese (Mn) levels, which are essential for plant growth, while also decreasing cadmium (Cd) accumulation. This study compares the Cd accumulation characteristics, yield, quality, and low-temperature tolerance of Shaoxiang100 and its original strain “44-5”. The multi-site experiments and demonstrations revealed that the strain “Shaoxiang 100” exhibited stable, low levels of Cd accu-mulation in rice grains. Additionally, the accumulation of Mn in plants decreased, but the yield remained unchanged when com-pared to the original strain “44-5”. Other main agronomic traits did not show significant differences, except for plant height. The fruiting rate of both Shaoxiang 100 and the original line “44-5” decreased significantly with the delay of sowing, and the plants also decreased. However, the yield remained the same as the original strain “44-5”, and there were no significant differences in the main agronomic traits, except for plant height. No changes in content have been made, as per the given instructions. The delay in sowing significantly reduced the fruiting rate of both Shaoxiang 100 and the original line “44-5”. Additionally, the relative cold tolerance coefficient and variety cold tolerance index were 0.75 and 0.75, respectively, indicating a decrease of 47.2% and 49.2%. Furthermore, the low-temperature tolerance at the spikelet stage was level 4, indicating sensitivity to cold. Compared to the original strain “44-5”, Shaoxiang 100 did not experience a decrease in Mn accumulation or low-temperature tolerance due to a mutation in the Os-NRAMP5 gene. The identification results indicate that both strains have general cold tolerance. Therefore, it is recommended to plant Shaoxiang 100 as double-crop late rice in Hunan and Jiangxi to prevent yield loss from low-temperature stress during the late growth period.