甜荞种质资源抗倒性评价及鉴定指标筛选
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作者单位:

1.定西市农业科学研究院,甘肃定西 743000;2.甘肃省农业科学院作物研究所,兰州 730070;3.安定区凤翔镇政府,甘肃定西 743000;4.成都大学食品与生物工程学院,成都 610106

作者简介:

研究方向为荞麦种质创新及遗传育种,E-mail: jrl101309@163.com

通讯作者:

向达兵,研究方向为特色杂粮作物高产栽培与营养生理生态,E-mail: dabing.xiang@163.com

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基金项目:

甘肃省科技计划(24CXNJ014); 农业农村部杂粮加工重点实验室/四川省杂粮产业化工程技术研究中心开放课题(2023CC002);国家燕麦荞麦产业技术体系(CARS-07-G-12)


Evaluation of Lodging Resistance and Selection of Identification Indexes of Common Buckwheat Germplasm Resources
Author:
Affiliation:

1.Dingxi Academy of Agricultural Sciences, Dingxi 743000,Gansu;2.Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070;3.Fengxiang Town Government, Anding District, Dingxi 743000,Gansu;4.College of Food and Biological Engineering, Chengdu University, Chengdu 610106

Fund Project:

Science and Technology Program of Gansu Province (24CXNJ014); The Opening Project of Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, and Sichuan Engineering and Technology Research Center of Coarse Cereal Industralization, Chengdu University(2023CC002); National Oats and Buckwheat Industry Technology System Program (CARS-07-G-12)

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    摘要:

    倒伏是影响甘肃中部甜荞产量的重要因素。本研究以 76份国内外甜荞种质资源为试验材料,通过计算 23个与抗倒伏密切相关的性状的遗传多样性,应用相关性分析、主成分分析、回归分析、聚类分析及逐步判别分析等方法,对甜荞种质资源抗倒性进行综合评价。结果表明,76份甜荞种质材料的遗传多样性较高,不同性状的遗传多样性指数分布范围为2.349~4.331,其中,主茎第二节间长最大,主茎分枝数最小。相关性分析表明,23个性状之间存在着不同程度的相关性。主成分分析表明,前 5个主成分能够代表76份甜荞种质 23 个性状81.421% 的信息量。利用隶属函数法计算甜荞种质资源抗倒性综合得分值(D 值),D值平均为 0.469,云甜荞1号D值最低(0.200),TQ10-07 D值最高(0.819)。通过逐步回归分析,筛选出株高、茎秆重心高度、主茎第一节间长及壁厚、主茎第二节间长、主茎分枝数、弯曲度和茎秆抗折力等8 个性状可作为甜荞种质资源抗倒性的综合评价指标。聚类分析将76份供试材料分为4个类群,其中类群Ⅳ的10份材料 D 值最高,抗倒伏能力最强,可作为种质创新及抗倒育种的亲本材料。本研究表明采用多元化统计分析方法对甜荞种质资源抗倒性进行综合评价是可行的,为甘肃中部地区甜荞种质创新和抗倒伏育种提供依据和借鉴。

    Abstract:

    Lodging is a main problem affecting the yield of common buckwheat in central Gansu, China. This study used 76 common buckwheat germplasm resources from both domestic and international sources to investigate genetic diversity of 23 phenotypic traits that associate to lodging resistance. Through applying statistic methods such as Shannon-Wiener diversity index, correlation analysis, principal component analysis, regression analysis, cluster analysis and stepwise discriminate analysis, a comprehensive evaluation of the lodging resistance was conducted. The collection of common buckwheat exhibited high genetic diversity, with diversity indices ranging from 2.349 to 4.331 for different traits, among which the number of main stem branches was the smallest and the length of the second inter-node was the highest. Correlation analysis revealed correlations of varying degrees among the 23 traits. The principal component analysis showed that the first five principal components could represent 81.421% of the information of the 23 traits. Using a membership function method, a comprehensive score value (D) for lodging resistance was calculated, with an average D value of 0.469. The D value for Yuntianqiao 1 was the lowest (0.200) and the highest for TQ10-07(0.819). Stepwise regression analysis identified eight traits, including plant height, stem height of center gravity, the first inter-node length and wall thickness, the second inter-node length, number of main stem branches, bending degree and stem resistance, as comprehensive evaluation indicators for lodging resistance of common buckwheat germplasm resources. Based on D value clustering, 76 materials were divided into four groups,among of which, 10 materials in class Ⅳ exhibited high lodging resistance and high D value, which could be used as parent materials for material innovation and breeding. This study showed that using a multivariate statistical analysis method for comprehensive evaluation of lodging resistance is feasible, providing a basis and reference for germplasm innovation and lodging-resistant breeding in common buckwheat in central Gansu, China.

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贾瑞玲,赵小琴,陈富,等.甜荞种质资源抗倒性评价及鉴定指标筛选[J].植物遗传资源学报,2025,26(1):120-132.

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  • 收稿日期:2024-03-14
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  • 在线发布日期: 2025-01-07
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