一个新的小麦黄叶位点的分子定位
作者:
作者单位:

山西农业大学农学院 / 作物遗传与分子改良山西省重点实验室

作者简介:

通讯作者:

中图分类号:

基金项目:

中央引导地方科技发展资金项目(YDZJSX20231A037);山西省基础研究计划青年科学研究项目(202203021212418)


Molecular Mapping of a Novel Yellow Leaf Locus in Wheat
Author:
Affiliation:

College of Agriculture,Shanxi Agricultural University / Shanxi Key Laboratory of Crop Genetics and Molecular Improvement

Fund Project:

S&T Development Foundation of Central Guides Local Government Project (YDZJSX20231A037);Basic Research Program of Shanxi Province (202203021212418)

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    小麦叶片颜色与植株光合效率和籽粒产量密切相关。人工合成小麦Y223表现为生理性黄叶,与正常绿叶品种晋作82的杂种F1植株叶色表现为中间型,在Y223旗叶抽出17 d后,杂种F2群体中绿叶、中间和黄叶类型植株数目的分离比例符合1:2:1,表明Y223的黄叶表现不完全显性。采用混合分离群体分析法,利用杂种F2代植株构建绿叶池和黄叶池,从分布于小麦21条染色体的569个SSR标记中筛选出12个在亲本间和绿叶/黄叶池间表现多态性的标记。利用9个位于2B染色体的多态性标记扩增F2群体,最终用5个标记定位了黄叶位点,暂命名为YL-2B,其侧翼标记为Xbarc200和Xbarc55,遗传距离分别为2.3 cM和3.8 cM。通过比较显隐性、黄叶表型和标记连锁程度,证明YL-2B与2B染色体上已报道黄叶位点均不相同,是1个新的黄叶位点。本研究初步明确了Y223黄叶位点的遗传规律和染色体位置,为下一步基因克隆以及叶片衰老机制解析奠定了基础。

    Abstract:

    Leaf color is closely related to plant photosynthetic efficiency and grain yield of wheat. The physiological yellow leaves were present in an artificially synthesized wheat Y223. The F1 plants derived from the cross between Y223 and a normal green leaf variety Jinzuo 82 displayed intermediate phenotype in leaf color. Seventeen days after the emergence of flag leaves, the segregation ratio for green leaf, intermediate, and yellow leaf types in the F2 population Y223 × Jinzuo 82 fits to 1:2:1. Using the bulked segregant analysis method, F2 individuals with contrasting phenotypes were selected to establish green leaf pools and yellow leaf pools. From 569 SSR markers distributed on 21 chromosomes of wheat, 12 markers with consistent polymorphism between parents and green/yellow leaf pools were identified. Nine of them were located on chromosome 2B. These markers were used to genotype the F2 population, and the yellow leaf locus, designated YL-2B, was mapped by five markers. It was flanked by markers Xbarc200 and Xbarc55, with genetic distances of 2.3 cM and 3.8 cM, respectively. By comparing the characteristic of dominance/recessiveness, yellow leaf phenotype, and marker linkage, YL-2B proved to differ from the reported yellow leaf loci on chromosome 2B, indicating that it is possibly a novel locus. This study clarifies the inheritance mode and chromosome position of the yellow leaf locus carried by Y223, which provides a reference information for gene cloning and understanding leaf senescence mechanism.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2024-03-18
  • 最后修改日期:2024-05-02
  • 录用日期:2024-08-13
  • 在线发布日期: 2024-08-13
  • 出版日期:
您是第位访问者
ICP:京ICP备09069690号-23
京ICP备09069690号-23
植物遗传资源学报 ® 2024 版权所有
技术支持:北京勤云科技发展有限公司