Leaf color is closely related to plant photosynthetic efficiency and grain yield of wheat. The physiological yellow leaves were present in an artificially synthesized wheat Y223. The F1 plants derived from the cross between Y223 and a normal green leaf variety Jinzuo 82 displayed intermediate phenotype in leaf color. Seventeen days after the emergence of flag leaves, the segregation ratio for green leaf, intermediate, and yellow leaf types in the F2 population Y223 × Jinzuo 82 fits to 1:2:1. Using the bulked segregant analysis method, F2 individuals with contrasting phenotypes were selected to establish green leaf pools and yellow leaf pools. From 569 SSR markers distributed on 21 chromosomes of wheat, 12 markers with consistent polymorphism between parents and green/yellow leaf pools were identified. Nine of them were located on chromosome 2B. These markers were used to genotype the F2 population, and the yellow leaf locus, designated YL-2B, was mapped by five markers. It was flanked by markers Xbarc200 and Xbarc55, with genetic distances of 2.3 cM and 3.8 cM, respectively. By comparing the characteristic of dominance/recessiveness, yellow leaf phenotype, and marker linkage, YL-2B proved to differ from the reported yellow leaf loci on chromosome 2B, indicating that it is possibly a novel locus. This study clarifies the inheritance mode and chromosome position of the yellow leaf locus carried by Y223, which provides a reference information for gene cloning and understanding leaf senescence mechanism.