2025年4月7日 7:31 星期一
首页 > 过刊浏览>2025年第26卷第1期 >133-147. DOI:10.13430/j.cnki.jpgr.20240403002
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内蒙古糜黍资源DNA分子身份证的构建
作者:
作者单位:

1.山西农业大学农学院，太谷030801;2.山西农业大学农业基因资源研究中心/农业农村部黄土高原作物基因资源与种质创制重点实验室/ 杂粮种质资源发掘与遗传改良山西省重点实验室，太原 030031;3.内布拉斯加大学林肯分校农艺系小宗粮豆研究与推广中心， 美国内布拉斯加州斯克茨布拉夫69361

作者简介:

研究方向为种质创新与遗传工程，E-mail： f164138146@163.com

通讯作者:

王瑞云，研究方向为糜子分子育种、种质创新与遗传工程，E-mail： wry925@126.com
乔治军，研究方向为旱作栽培与作物生理、种质资源创新与利用，E-mail： nkypzs@126.com

基金项目:

国家现代农业产业技术体系建设专项（CARS-06-14.5-A16）；山西省现代农业产业技术体系建设专项资金项目（2024CYJSTX03-12）；山西省重点研发项目（2022ZDYF110）；山西省“1331”工程—作物学—流学科建设项目；山西农业大学农学院研究生教育改革与质量提升工程项目（2023YCX33，2023YCX48，2023YCX45，2023YDT05）


Construction of DNA Molecular Identity Card of Broomcorn Millet Resources in Inner Mongolia
Author:
Affiliation:

1.College of Agronomy， Shanxi Agricultural University， Taigu030801;2.Center for Agricultural Genetic Resources Research， Shanxi Agricultural University/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau， Ministry of Agriculture and Rural Affairs/Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops， Taiyuan 030031;3.Panhandle Research & Extension Center， Department of Agronomy and Horticulture， University of Nebraska-Lincoln， Scottsbluff 69361， Nebraska， USA

Fund Project:

National Special Fund for Construction of Modern Agricultural Industrial Technology System （CARS-06-14.5-A16）； The Special Fund for Construction of Modern Agricultural Industrial Technology System of Shanxi Province （2023CYJSTX03-12）； The Key Research & Development Project of Shanxi Province （2022ZDYF110）；Shanxi “1331”Project Crop Soience First-Class Discipline Construction Project； Graduate Education Reform and Quality Improvement Project of College of Agronomy， Shanxi Agricultural University （2023YCX33，2023YCX48，2023YCX45，2023YDT05）

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    摘要:

    糜黍（Panicum miliaceum L.）是一种品种资源多样的古老作物，采用荧光SSR标记，对糜黍资源进行数字化的管理与分类，可将众多资源合理高效应用。本研究以258份内蒙古糜黍资源为材料，基于本课题组前期开发的85个常规SSR标记，经多次扩增筛选、优化、加工（引物5′端添加荧光基团）获得12个荧光SSR核心标记。利用MapChart 2.32绘制标记的染色体定位图，利用ID Analysis 4.0检测标记对材料的区分度，用PowerMarker 3.25和PopGen 1.32进行遗传多样性分析，用 MEGA 11.0.10和NTSYSpc2.11a分别绘制聚类图和主成分分析图，最终应用草料二维码生成器构建材料的DNA分子身份证。遗传多样性分析发现，利用7个标记扩增258份材料，共有123个等位基因，平均每个标记有17.5714个；有效等位基因数、Shannon多样性指数、观测杂合度、期望观测杂合度、Nei′s基因多样性指数和多态性信息含量均值分别为7.4622、2.2270、0.8021、0.8372、0.8353和0.8994。将毛细管电泳结果以特定方式进行编码处理，最终仅用7个荧光标记（RYW6、RYW8、RYW37、RYW40、RYW67、RYW124和RYW125）组合在一起生成全部材料的字符串和二维码DNA分子身份证，为内蒙古糜黍种质资源的分类管理及快速鉴定提供了分子检测工具和理论依据。

    Abstract:

    Broomcorn millet （Panicum miliaceum L.） is a diverse and ancient crop with rich germplasm resources. The use of fluorescent SSR markers enables the digital management and classification of these resources， facilitating their rational and efficient utilization. In this study， 258 broomcorn millet accessions from Inner Mongolia， China， were used to develop 12 fluorescent SSR markers， based on 85 pairs of conventional SSR markers previously developed by our research group， through multiple rounds of amplification， selection， optimization and modification （adding fluorescent labels to the 5'-end of the primers）. MapChart 2.32 was used to plot the chromosome location of these markers， ID Analysis 4.0 to assess the discriminating power of the markers， PowerMarker 3.25 and PopGen 1.32 for genetic diversity analysis， MEGA 11.0.10 for constructing the cluster diagram， and NTSYSpc2.11a for principal component analysis. A DNA molecular ID was created for the genotypes using a QR code generator. Genetic diversity analysis showed that there were 123 alleles in 258 materials amplified by 7 pairs of markers， with an average of 17.5714 alleles per marker. The mean values of effective alleles ， Shannon diversity index， Observed heterozygosity， expected heterozygosity ， Nei's gene diversity index and Polymorphism information content were 7.4622， 2.2270， 0.8021， 0.8372， 0.8353， and 0.8994. The results of capillary electrophoresis were encoded in a specific way， and only 7 pairs of fluorescent labels （RYW6， RYW8， RYW37， RYW40， RYW67， RYW124 and RYW125） were used to generate 258 strings and two-dimensional code DNA molecular identity cards of Inner Mongolia millet resources. This approach provided a molecular detection tool and theoretical basis for the classification management and rapid identification of the germplasm resources of Inner Mongolia broomcorn millet germplasm resources.

    参考文献
    [1] Crawford G W. Agricultural origins in North China pushed back to the Pleistocene Holocene boundary. Proceedings of the National Academy of Sciences of the USA, 2009, 106 (18): 7271-7272
    [2] 柴岩. 糜子. 北京: 中国农业出版社, 1999: 71-86Chai Y. Broomcorn millet (Panicum miliaceum L.). Beijing: China Agriculture Press, 1999: 71-86
    [3] Yang Q H, Zhang P P, Qu Y, Gao X L, Liang J B, Yang P, Feng B L. Comparison of physicochemical properties and cooking edibility of waxy and non-waxy proso millet (Panicum miliaceum L.). Food Chemistry, 2018, 257: 271-278
    [4] Yue H, Wang M, Liu S Y, Du X H, Song W N, Nie X J. Transcriptome-wide identification and expression profiles of the WRKY transcription factor family in broomcorn millet (Panicum miliaceum L.). BMC Genomics, 2016, 17 (1): 343
    [5] Shi J P, Ma X X, Zhang J H, Zhou Y S, Liu M X, Huang L L, Sun S L, Zhang X B, Gao X, Zhan W, Li P H, Wang L, Lu P, Zhao H M, Song W B, Lai J S. Chromosome conformation capture resolved near complete genome assembly of broomcorn millet. Nature Communications, 2019,10 (1): 464
    [6] 林元香. 山西糜子核心种质分子身份证的构建. 太谷: 山西农业大学, 2022Lin Y X. Construction of molecular ID card of core germplasm of common millet (Panicum miliaceum L.). Taigu: Shanxi Agricultural University, 2022
    [7] 牟泉霖, 孙永刚, 张红星. 内蒙古金代两家子堡址植物浮选结果及分析. 赤峰学院学报: 汉文哲学社会科学版, 2023, 44 (9): 1-5Mou Q L, Sun Y G, Zhang H X. Flotation results and analysis of plants in Liangjiazi fort site of Jin dynasty in Inner Mongolia. Journal of Chifeng University: Philosophy and Social Science Chinese Edition, 2023, 44 (9): 1-5
    [8] 孙永刚, 田小冬, 塔拉, 岳够明. 元上都西关厢遗址植物遗存综合研究. 中国农史, 2017, 36 (5): 12-24Sun Y G, Tian X D, Ta L, Yue G M. Flotation results from the Xiguanxiang site belongs to the upper capital of the Yuan dynasty. Agricultural History of China, 2017, 36 (5): 12-24
    [9] 刘玮, 赵志军, 霍东峰, 朱永刚. 内蒙古库伦旗三家子遗址浮选结果分析报告. 农业考古, 2016 (3): 7-13Liu W, Zhao Z J, Huo D F, Zhu Y G. Flotation results and analysis from Sanjiazi site of Kulun Banner in Inner Mongolia. Agricultural Archaeology, 2016 (3): 7-13
    [10] 孙永刚, 刘晓辰. 内蒙古库伦小奈林稿遗址浮选出土植物遗存分析. 北方文物, 2019 (3): 50-53,110Sun Y G, Liu X C. Analysis of plant remains unearthed by flotation at the Xiaonailingao site of Kulun in Inner Mongolia. Northern Cultural Relics, 2019 (3): 50-53,110
    [11] 孙永刚, 赵志军, 吉平. 哈民忙哈史前聚落遗址出土植物遗存研究. 华夏考古, 2016 (2): 45-52Sun Y G, Zhao Z J, Ji P. Study on the plant remains unearthed from the prehistoric settlement site of Haminmangha. Huaxia Archaeology, 2016 (2): 45-52
    [12] 刘杰, 孙永刚, 张红星. 内蒙古下田家营子夏家店上层文化遗址出土植物遗存研究. 赤峰学院学报: 汉文哲学社会科学版, 2022, 43 (3): 90-93Liu J, Sun Y G, Zhang H X. Study on the excavated plant remains from the upper Xiajiadian culture in Xiatianjiayingzi site. Journal of Chifeng University: Philosophy and Social Science Chinese Edition, 2022, 43 (3): 90-93
    [13] 孙永刚. 巴彦塔拉辽代遗址植物遗存及相关问题研究. 赤峰学院学报: 汉文哲学社会科学版, 2013 (8): 7-10Sun Y G. Study on plant remains and related issues of Bayantala Liao dynasty site. Journal of Chifeng University: Philosophy and Social Science Chinese Edition, 2013 (8): 7-10
    [14] 孙永刚, 李阳, 格日乐图. 内蒙古小塘山遗址2021年度植物浮选结果及分析. 赤峰学院学报: 汉文哲学社会科学版, 2022, 43 (3): 78-83Sun Y G, Li Y, Ge R L T. Plant flotation results and analysis on the Xiaotangshan site of Inner Mongolia in 2021. Journal of Chifeng University: Philosophy and Social Science Chinese Edition, 2022, 43 (3): 78-83
    [15] 张帅, 田惠农, 孙永刚. 内蒙古敖汉热水汤遗址浮选结果分析. 农业考古, 2016 (6): 22-27Zhang S, Tian H N, Sun Y G. Analysis of flotation results of Reshuitang site of Aohan in Inner Mongolia. Agricultural Archaeology, 2016 (6): 22-27
    [16] 孙永刚, 赵志军, 曹建恩, 孙金松, 党郁. 内蒙古二道井子遗址2009年度浮选结果分析报告. 农业考古, 2014 (6): 1-9Sun Y G, Zhao Z J, Cao J E, Sun J S, Dang Y. Analysis report of flotation results of Erdaojingzi site in Inner Mongolia in 2009. Agricultural Archaeology, 2014 (6): 1-9
    [17] 文占平. 内蒙古自治区杂粮杂豆的生产、加工与销售. 现代农业, 2001 (4): 1-4Wen Z P. Production, processing and sales of grains and beans in Inner Mongolia. Modern Agriculture, 2001 (4): 1-4
    [18] 王瑞云. 糜子遗传多样性及进化研究进展. 北京: 中国农业出版社, 2017: 2-3Wang R Y. Genetic diversity and evolution advancement in common millet (Panicum miliaceum L.). Beijing: China Agriculture Press, 2017: 2-3
    [19] Wang R Y, Hunt H V, Qiao Z J, Wang L, Han Y H. Diversity and cultivation of broomcorn millet (Panicum miliaceum L.) in China: A review. Economic Botany, 2016, 70 (3): 332-342
    [20] 正月, 张杰, 琦明玉, 李志光, 于凤艳, 张富丽, 张立媛. 内蒙古糜子主产区品种丰产、稳产及适应性分析. 种子, 2018, 37 (12): 110-113Zheng Y, Zhang J, Qi M Y, Li Z G, Yu F Y, Zhang F L, Zhang L Y. Analysis on high yield, stable yield and adaptability of varieties in main producing areas of Inner Mongolia glutinous millet. Seed, 2018, 37 (12): 110-113
    [21] 门果桃, 刘建设, 任永峰, 刘锦川, 杨文耀, 张小平, 张新功. 内蒙古糜子品种资源研究初报. 内蒙古农业科技, 2012 (5): 23Men G T, Liu J S, Ren Y F, Liu J C, Yang W Y, Zhang X P, Zhang X G. Preliminary study on broomcorn millet variety resources in Inner Mongolia. Inner Mongolia Agricultural Science And Technology, 2012 (5): 23
    [22] 陈昌文, 曹珂, 王力荣, 朱更瑞, 方伟超. 中国桃主要品种资源及其野生近缘种的分子身份证构建. 中国农业科学, 2011, 44 (10): 2081-2093Chen C W, Cao K, Wang L R, Zhu G R, Fang W C. Molecular ID establishment of main China peach varieties and peach related species. Scientia Agricultura Sinica, 2011, 44 (10): 2081-2093
    [23] 门果桃, 陈强, 范挨计, 门永亮, 任龙梅. 内蒙古糜子产业发展现状与对策. 内蒙古农业科技, 2009 (2): 79-82Men G T, Chen Q, Fan A J, Men Y L, Ren L M. The development status and countermeasures of broomcorn millet industry in Inner Mongolia. Inner Mongolia Agricultural Science and Technology, 2009 (2): 79-82
    [24] 赵敏, 李书田, 于占斌, 于学文, 李艳红. 内蒙古糜子资源与综合利用. 内蒙古农业科技, 2007 (6): 101-102Zhao M, Li S T, Yu Z B, Yu X W, Li Y H. Inner Mongolia broomcorn millet resources and comprehensive utilization. Inner Mongolia Agricultural Science and Technology, 2007 (6): 101-102
    [25] 冯智尊, 杨雅斐, 王海岗, 陈凌, Santra Dipak K, 王瑞云, 乔治军. 山西糜黍遗传差异评估及分子身份证构建. 山西农业科学, 2023, 51 (10): 1178-1188Feng Z Z, Yang Y F, Wang H G, Chen L, Santra D K, Wang R Y, Qiao Z J. Assessment of genetic differences and construction of molecular identity cards of broomcorn millet in Shanxi. Journal of Shanxi Agricultural Sciences, 2023, 51 (10): 1178-1188
    [26] Cho Y I, Chung J W, Lee G A, Ma K H, Dixit A, Gwag J G, Park Y J. Development and characterization of twenty-five new polymorphic microsatellite markers in proso millet (Panicum miliaceum L.). Genes Genomics, 2010, 32 (3): 267-273
    [27] Hunt H V, Campana M G, Lawes M C, Park Y J, Bower M A, Howe C J, Jones M K. Genetic diversity and phylogeography of broomcorn millet (Panicum miliaceum L.) across Eurasia. Molecular Ecology, 2011, 20 (22): 4756-4771
    [28] Hu X Y, Wang J F, Lu P, Zhang H S. Assessment of genetic diversity in broomcorn millet (Panicum miliaceum L.) using SSR markers. Journal of Genetics and Genomics, 2009, 36 (8): 491-500
    [29] 刘笑瑜. 利用高基元SSR分析中国糜子资源的遗传多样性. 太谷: 山西农业大学, 2019Liu X Y. Analysis of genetic diversity of Chinese proso millet resources by high substrate SSR. Taigu: Shanxi Agricultural University, 2019
    [30] 薛亚鹏, 丁艺冰, 王宇卓, 王晓丹, 曹晓宁, Santra Dipak K, 陈凌, 乔治军, 王瑞云. 基于荧光SSR构建中国糜子核心种质DNA分子身份证. 中国农业科学, 2023, 56 (12): 2249-2277Xue Y P, Ding Y B, Wang Y Z, Wang X D, Cao X N, Santra D K, Chen L, Qiao Z J, Wang R Y. Construction of DNA molecular identity card of core germplasm of broomcorn millet in China based on fluorescence SSR. Scientia Agricultura Sinica, 2023, 56 (12): 2249-2277
    [31] 赵海娟, 章秋平, 马小雪, 刘宁, 张玉萍, 张玉君, 徐铭, 刘家成, 刘威生, 刘硕. 杏高通量荧光SSR检测体系及分子身份证的构建. 分子植物育种, 2023, 21 (24): 8134-8143Zhao H J, Zhang Q P, Ma X X, Liu N, Zhang Y P, Zhang Y J, Xu M, Liu J C, Liu W S, Liu S. The establishment of fluorescent SSR markers for high-throughput detection and construction of molecular identity on Apricot. Molecular Plant Breeding, 2023, 21 (24): 8134-8143
    [32] 安苗, 王彤彤, 付逸婷, 夏俊俊, 彭锁堂, 段永红. 52个马铃薯遗传多样性分析及SSR分子身份证构建. 生物技术通报, 2023, 39 (12): 136-147An M, Wang T T, Fu Y T, Xia J J, Peng S T, Duan Y H. Genetic diversity analysis and molecular identity card construction by SSR markers of 52 Solanum tuberosum L. varieties(lines). Biotechnology Bulletin, 2023, 39 (12): 136-147
    [33] 王宇卓, 林元香, 薛亚鹏, 段政勇, 王晓丹, 陈凌, 曹晓宁, 王瑞云, 乔治军. 山西糜子核心种质分子身份证构建. 植物学报, 2023, 58 (1): 159-168Wang Y Z, Lin Y X, Xue Y P, Duan Z Y, Wang X D, Chen L, Cao X N, Wang R Y, Qiao Z J. Construction of molecular ID card of core germplasm of hog millet (Panicum miliaceum) in Shanxi. Chinese Bulletin of Botany, 2023, 58 (1): 159-168
    [34] 陈小红, 林元香, 王倩, 丁敏, 王海岗, 陈凌, 高志军, 王瑞云, 乔治军. 基于高基元SSR构建黍稷种质资源的分子身份证. 作物学报, 2022, 48 (4): 908-919Chen X H, Lin Y X, Wang Q, Ding M, Wang H G, Chen L, Gao Z J, Wang R Y, Qiao Z J. Development of DNA molecular ID card in hog millet germplasm based on high motif SSR. Acta Agronomica Sinica, 2022, 48 (4): 908-919
    [35] 丁艺冰, 辛旭霞, 冯智尊, 曹越, 郭娟, Santra Dipak K, 王瑞云, 陈喜明. 东北春播区糜子核心种质及其DNA分子身份证构建. 作物学报, 2024, 50 (5): 1181-1192Ding Y B, Xin X X, Feng Z Z, Cao Y, Guo J, Santra D K, Wang R Y, Chen X M. Core germplasm and DNA molecular identity card of proso millet in Northeast Spring sowing region in China. Acta Agronomica Sinica, 2024, 50 (5): 1181-1192
    [36] 冯琬淇, 张福顺, 刘乃新. 毛细管与聚丙烯酰胺凝胶电泳检测甜菜SSR位点的比较研究. 中国农学通报, 2022, 38 (36): 34-41Feng W Q, Zhang F S, Liu N X. SSR polymorphism in sugar beet:Detection by polyacrylamide gel electrophoresis and capillary electrophoresis. Chinese Agricultural Science Bulletin, 2022, 38 (36): 34-41
    [37] 刘晓鑫, 谢传晓, 赵琦, 路明, 曲延英, 张滨, 梁业红, 张世煌. 基于SSR荧光标记技术的玉米群体混合样本基因频率分析方法. 中国农业科学, 2008, 41 (12): 3991-3998Liu X X, Xie C X, Zhao Q, Lu M, Qu Y Y, Zhang B, Liang Y H, Zhang S H. Establishment of fluorescent SSR technique on detecting allelic frequency in maize (Zea mays L.) populations with bulk sampling strategy. Scientia Agricultura Sinica, 2008, 41 (12): 3991-3998
    [38] 郑德波, 石前, 蒙平, 庾韦花. 基于SSR荧光标记的广西天冬种质资源遗传多样性分析. 种子, 2023, 42 (9): 98-104Zheng D B, Shi Q, Meng P, Yu W H. Genetic diversity analysis of Asparagus cochinchinensis (Lour.) Merr. germplasm resources in Guangxi based on fluorescent SSR markers. Seed, 2023, 42 (9): 98-104
    [39] 唐玉娟, 罗世杏, 黄国弟, 宋恩亮, 李日旺, 赵英, 张宇, 莫永龙, 唐莹莹. 基于SSR荧光标记的杧果种质资源遗传多样性分析及分子身份证构建. 热带作物学报, 2023, 44 (11): 2292-2304Tang Y J, Luo S X, Huang G D, Song E L, Li R W, Zhao Y, Zhang Y, Mo Y L, Tang Y Y. Genetic diversity analysis and molecular ID construction of Mango germplasm based on SSR fluorescence markers. Chinese Journal of Tropical Crops, 2023, 44 (11): 2292-2304
    [40] 王瑞云, 季煦, 陆平, 刘敏轩, 许月, 王纶, 王海岗, 乔治军. 利用荧光SSR分析中国糜子遗传多样性. 作物学报, 2017, 43 (4): 530-548Wang R Y, Ji X, Lu P, Liu M X, Xu Y, Wang L, Wang H G, Qiao Z J. Analysis of genetic diversity in common millet (Panicum miliaceum) using fluorescent SSR in China. Acta Agronomica Sinica, 2017, 43 (4): 530-548
    [41] Edwards K, Johnstone C, Thompson C. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Research, 1991, 19 (6): 1349
    [42] 何杰丽, 石甜甜, 陈凌, 王海岗, 高志军, 杨美红, 王瑞云, 乔治军. 糜子EST-SSR分子标记的开发及种质资源遗传多样性分析. 植物学报, 2019, 54 (6): 723-732He J L, Shi T T, Chen L, Wang H G, Gao Z J, Yang M H, Wang R Y, Qiao Z J. The genetic diversity of common millet (Panicum miliaceum) germplasm resources based on the EST-SSR markers. Chinese Bulletin of Botany, 2019, 54 (6): 723-732
    [43] 王瑞云, 刘笑瑜, 王海岗, 陆平, 刘敏轩, 陈凌, 乔治军. 用高基元微卫星标记分析中国糜子遗传多样性. 中国农业科学, 2017, 50 (20): 3848-3870Wang R Y, Liu X Y, Wang H G, Lu P, Liu M X, Chen L, Qiao Z J. Evaluation of genetic diversity of common millet (Panicum miliaceum) germplasm available in China using high motif nucleotide repeat SSR markers. Scientia Agricultura Sinica, 2017, 50 (20): 3848-3870
    [44] 薛亚鹏, 李元阳, 陈凌, 王海岗, 王瑞云, 乔治军. 山西省糜子DNA分子身份证的构建. 山西农业大学学报: 自然科学版, 2022, 42 (1): 19-25Xue Y P, Li Y Y, Chen L, Wang H G, Wang R Y, Qiao Z J. Construction of DNA molecular identity card of broomcorn millet germplasm in Shanxi province. Journal of Shanxi Agricultural University: Natural Science Edition, 2022, 42 (1): 19-25
    [45] Liu K J, Muse S V. PowerMarker: An integrated analysis environment for genetic marker data. Bioinformatics, 2005, 21 (9): 2128-2129
    [46] Yeh F C, Boyle T J B. Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgian Journal of Botany, 1996, 129: 157
    [47] Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Molecular Biology and Evolution, 2011, 28 (10): 2731-2739
    [48] 吴跃进, 陶亮之, 马伯军, 陈析丰, 刘斌美. 水稻早衰基因ESL8的遗传与定位. 作物学报, 2023, 49 (3): 662-671Wu Y J, Tao L Z, Ma B J, Chen X F, Liu B M. Heredity and fine mapping of an early-senescence leaf gene ESL8 in rice. Acta Agronomica Sinica, 2023, 49 (3): 662-671
    [49] 付美玉, 熊宏春, 周春云, 郭会君, 谢永盾, 赵林姝, 古佳玉, 赵世荣, 丁玉萍, 徐延浩, 刘录祥. 小麦矮秆突变体je0098的遗传分析与其矮秆基因定位. 作物学报, 2022, 48 (3): 580-589Fu M Y, Xiong H C, Zhou C Y, Guo H J, Xie Y D, Zhao L S, Gu J Y, Zhao S R, Ding Y P, Xu Y H, Liu L X. Genetic analysis of wheat dwarf mutant je0098 and molecular mapping of dwarfing gene. Acta Agronomica Sinica, 2022, 48 (3): 580-589
    [50] 周向阳, 赵亮, 狄佳春, 陈旭升. 2个抗虫棉的外源Bt基因分子鉴定及其染色体定位. 作物学报, 2019, 45 (9): 1440-1445Zhou X Y, Zhao L, Di J C, Chen X S. Molecular identification and chromosomal mapping of exogenous Bt gene in two insect-resistant cotton varieties. Acta Agronomica Sinica, 2019, 45 (9): 1440-1445
    [51] 李风. 玉米染色体片段代换系的构建及产量相关性状QTL定位. 武汉: 华中农业大学, 2013Li F. Development of a set of chromosome segment substitution lines of maize and mapping QTL for yield related traits. Wuhan: Huazhong Agricultural University, 2013
    [52] 陈小红, 何杰丽, 石甜甜, 邵欢欢, 王海岗, 陈凌, 高志军, 王瑞云, 乔治军. 基于转录组测序开发糜子SSR标记. 中国农业科学, 2020, 53 (10): 1940-1949Chen X H, He J L, Shi T T, Shao H H, Wang H G, Chen L, Gao Z J, Wang R Y, Qiao Z J. Developing SSR markers of proso millet based on transcriptome sequencing. Scientia Agricultura Sinica, 2020, 53 (10): 1940-1949
    [53] 段政勇, 邢志, 王海岗, 陈凌, Santra Dipak K, 王瑞云, 乔治军. 用微卫星标记分析北方春糜子区黍稷的遗传差异. 山西农业科学, 2022, 50 (4): 494-503Duan Z Y, Xing Z, Wang H G, Chen L, Santra D K, Wang R Y, Qiao Z J. Analysis of genetic differences of broomcorn millet in Northern spring-sowing ecotope by microsatellite markers. Journal of Shanxi Agricultural Sciences, 2022, 50 (4): 494-503
    [54] 王舒婷, 何杰丽, 石甜甜, 陈凌, 王海岗, 王瑞云, 乔治军. 利用微卫星标记分析山西糜子的遗传多样性. 植物遗传资源学报, 2019, 20 (1): 69-78Wang S T, He J L, Shi T T, Chen L, Wang H G, Wang R Y, Qiao Z J. Genetic diversity analysis of broomcorn millet (Panicum miliaceum L.) of Shanxi province using microsatellite markers. Journal of Plant Genetic Resources, 2019, 20 (1): 69-78
    [55] 陈雅琼. 基于SSR荧光标记和毛细管电泳检测技术的我国烟草审(认)定品种遗传多样性研究. 北京: 中国农业科学院, 2011Chen Y Q. The genetic diversity of tobacco varieties certificated in China based on fluorescent SSR and capillary electrophoresis. Beijing: Chinese Academy of Agricultural Sciences, 2011
    [56] 赵久然, 王凤格, 郭景伦, 陈刚, 廖琴, 孙世贤, 陈如明, 刘龙洲. 中国玉米新品种DNA指纹库建立系列研究II．适于玉米自交系和杂交种指纹图谱绘制的SSR核心引物的确定. 玉米科学, 2003, 11 (2): 3-5Zhao J R, Wang F G, Guo J L, Chen G, Liao Q, Sun S X, Chen R M, Liu L Z. Series of research on establishing DNA fingerprinting pool of Chinese new maize cultivars II. Confirmation of a set of SSR core primer pairs. Journal of Maize Sciences, 2003, 11 (2): 3-5
    [57] 李红琴, 刘宝龙, 张波, 张怀刚. 青海省审定小麦品种SSR遗传多样性分析及分子身份证的建立. 作物杂志, 2020 (3): 60-65Li H Q, Liu B L, Zhang B, Zhang H G. Analysis of genetic diversity and establishment of molecular ID of the wheat cultivars registered in Qinghai using SSR. Crops, 2020 (3): 60-65
    [58] 李春花, 陈蕤坤, 王艳青, 尹桂芳, 卢文洁, 孙道旺, 吴斌, 王莉花. 利用SSR标记构建云南苦荞种质资源分子身份证. 分子植物育种, 2019, 17 (5): 1575-1582Li C H, Chen R K, Wang Y Q, Yin G F, Lu W J, Sun D W, Wu B, Wang L H. Establishment of the molecular ID for Yunnan tartary buckwheat germplasm resources based on SSR marker. Molecular Plant Breeding, 2019, 17 (5): 1575-1582
    [59] 胡玉璐, 姜百灵, 陈凌, 王海岗, 曹晓宁, 王瑞云, 乔治军. 利用SSR创建中国北方黍稷资源DNA身份证. 山西农业科学, 2022, 50 (4): 478-485Hu Y L, Jiang B L, Chen L, Wang H G, Cao X N, Wang R Y, Qiao Z J. Using SSR to create DNA identity cards of broomcorn millet resources in Northern China. Journal of Shanxi Agricultural Sciences, 2022, 50 (4): 478-485
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冯智尊,辛旭霞,曹越,等.内蒙古糜黍资源DNA分子身份证的构建[J].植物遗传资源学报,2025,26(1):133-147.

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