玉米萌发期耐盐性评价及杂种优势模式分析
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1.吉林农业大学农学院;2.中国科学院植物研究所;3.中国农业科学院作物科学研究所

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国家重点研发计划


Evaluation of Salt Tolerance and Analysis of Heterosis Patterns in Maize during Germination
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1.College of Agronomy, Jilin Agricultural University;2.Institute of Botany, Chinese Academy of Sciences;3.Institute of Crop Science, Chinese Academy of Agricultural Sciences

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National key research and development program of China

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    摘要:

    摘要:为评价不同玉米材料萌发期对盐胁迫的耐受能力,探讨玉米杂交种材料萌发期耐盐性的杂优模式。本研究采用160 mmol/L的NaCl溶液对152份玉米自交系和303份杂交种进行盐胁迫处理,同时以蒸馏水培养为对照,通过培养皿纸床培养法进行萌发期耐盐性鉴定。结果显示,盐胁迫处理显著降低了玉米材料的发芽势、发芽率和发芽指数。性状相关性分析发现,不同性状之间存在极显著的正相关。采用萌发期耐盐率,并结合聚类分析结果,将自交系和杂交种材料分别划分五个耐盐级别:I级(高耐)、II级(耐)、III级(中等)、IV级(敏感)和V级(高感)。根据IBD( identity-by-descent )分析结果,将146份自交系材料划分为8个类群,不同类群自交系材料耐盐性表现为:EUROM群>Complex群>ZI330群>PB群>LAN群>SPT群>PA群>REID群。杂交种材料根据父母本材料类群的不同划分为23个杂种优势模式,其中EUROM×PB为萌发期对盐胁迫耐受性的最优杂种优势模式。本研究对自交系和杂交种材料进行耐盐性鉴定,筛选得到4份高耐盐自交系材料和17份高耐盐杂交种材料;初步确定了EUROM群自交系具有较强的耐盐性,以及最佳的杂种优势模式为EUROM×PB。

    Abstract:

    Abstract: To evaluate the tolerance of different maize materials to salt stress during the germination period and explore the heterozygous patterns of salt tolerance in maize hybrid materials during the germination period. This study used a 160 mmol/L NaCl solution to treat 152 maize inbred lines and 303 hybrid varieties under salt stress. Distilled water culture was used as the control, and salt tolerance during germination was identified using a paper bed culture method in a culture dish. The results showed that salt stress treatment significantly reduced the germination potential, germination rate, and germination index of maize materials. Trait correlation analysis revealed a highly significant positive correlation between different traits. Using the salt tolerance rate during the germination period and combining with the clustering analysis results, the inbred and hybrid materials were divided into five salt tolerance levels: Level I (high tolerance), Level II (tolerance), Level III (moderate), Level IV (sensitive), and Level V (high sensitivity). According to the results of IBD (identity by descent) analysis, 146 inbred line materials were divided into 8 groups. The salt tolerance of different groups of inbred line materials was as follows: EUROM group>Complex group>ZI330 group>PB group>LAN group>SPT group>PA group>REID group. The hybrid materials are divided into 23 heterosis patterns based on the different parent material groups, among which EUROM × PB is the optimal heterosis pattern for salt stress tolerance during germination. This study identified the salt tolerance of inbred lines and hybrid materials, and selected 4 high salt tolerant inbred line materials and 17 high salt tolerant hybrid materials; It has been preliminarily determined that the EUROM group inbred lines have strong salt tolerance, and the optimal heterosis pattern is EUROM × PB.

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  • 收稿日期:2024-04-05
  • 最后修改日期:2024-04-26
  • 录用日期:2024-09-19
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