郁金香花器官表型性状多样性分析
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青海大学农牧学院

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中国科学院“西部之光”项目(中科人字[2022]4号);青海省“昆仑英才”科技领军人才培养项目(青人才字[2022]1号);青海大学农牧学院大学生科研训练项目(NKX202301)


Diversity Analysis of Floral Organ Phenotypic Traits in Tulipa gesneriana L.
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Agriculture and Animal Husbandry College,Qinghai University,Xining

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"Light in the West" Projects of the Chinese Academy of Sciences ([2012]4);Qinghai Province "Kunlun Talent" Science and Technology Leading Talent Training Projects ([2022]1);Student Research Training Program at the College of Agriculture and Animal Husbandry, Qinghai University (NKX202301)

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    摘要:

    为探究郁金香种质资源花器官表型性状的多样性,以60个郁金香品种为试验材料,对9个花器官表型性状进行主成分分析、聚类分析,研究其花器官表型性状的遗传多样性,为郁金香种质资源的评价鉴定与利用提供参考依据。结果表明:60个郁金香品种的5个数量性状(花冠纵径、花冠横径、柱头长度、柱头周长和柱头直径)和4个质量性状(花冠主色、花粉颜色、柱头形状和始花期)均存在不同程度的差异。花冠纵径变异系数为19.35%,花冠横径变异系数为23.41%,品种间的变异范围较大,离散程度相对较高,而在柱头长度、周长和直径方面变异范围较小,分布集中且离散程度相对较低。91.67%的供试品种柱头形状为三棱柱,有5个品种出现柱头形状变异的现象。根据始花期的观测,供试品种中早花、中花、晚花的比例分别为20%、38%、12%。主成分分析共提取到可以反映郁金香花器官各表型性状的5个主成分,累计贡献率为85.179%。通过构建郁金香表型性状综合评价模型,综合得分>0.5的共有12个品种,其中‘糖果王子’、‘狡猾狐步舞’得分最高,属于2个粉色系中花品种,柱头长且粗,花器官综合性状表现优异。对60个郁金香品种的9个花器官表型性状进行主成分聚类分析,可将60个郁金香品种分为4大类,第I类的51个品种主要以花型较大且颜色艳丽、柱头较粗且花器官综合性状表现优异的郁金香品种为主;第II类仅‘紫色梦想’1个品种,在花器官的表现特点为花冠细长且柱头粗长;第III类聚集了6个柱头短小且花器官性状综合表现一般的品种;第IV类是柱头形状变异的2个品种。研究结果可从形态学方面为郁金香品种的分类及其亲缘关系的演化提供支撑,为进一步开发郁金香的优异花器官观赏性状、种质创新和品种改良提供参考依据。

    Abstract:

    To explore the diversity of floral organ phenotypic traits in tulip germplasm resources, 60 tulip cultivars were used as experimental materials. Principal component analysis and cluster analysis were performed on 9 floral organ phenotypic traits to study the genetic diversity of their floral organ phenotypic traits, providing reference for the evaluation, identification, and utilization of tulip germplasm resources. The results showed that there were varying degrees of differences in 5 quantitative traits (corolla longitudinal diameter, corolla transverse diameter, stigma length, stigma circumference, and stigma diameter) and 4 quality traits (corolla main color, pollen color, stigma shape, and initial flowering period). The coefficient of variation of corolla longitudinal diameter is 19.35%, and the coefficient of variation of corolla transverse diameter is 23.41%. The range of variation between cultivars is relatively large, and the degree of dispersion is relatively high. However, in terms of stigma length, circumference, and diameter, the range of variation is small, and the distribution is concentrated and the degree of dispersion is relatively low. 91.67% of the tested varieties have a triangular prism shaped stigma, and 5 cultivars exhibit variation in stigma shape. According to the observation of the initial flowering period, the proportions of early flowering, middle flowering, and late flowering in the tested cultivars are 20%, 38%, and 12%, respectively. Principal component analysis extracted 5 principal components that can reflect the phenotypic traits of tulip flower organs, with a cumulative contribution rate of 85.179%. By constructing a comprehensive evaluation model for the phenotypic traits of tulips, a total of 12 cultivars were found to have a comprehensive score (>0.5). Among them, 'Candy Prince' and 'Foxy Foxtrot' scored the highest, belonging to the two pink mid flowering cultivars. The stigma was long and thick, and the comprehensive traits of the flower organs were excellent. Principal component clustering analysis was conducted on 9 phenotypic traits of flower organs in 60 tulip cultivars, which can be divided into 4 categories. The 51 cultivars in the first category are mainly tulip cultivars with larger and more colorful flower types, thicker stigma, and excellent comprehensive performance of flower organ traits; The second type is only one variety of 'Purple Dream', characterized by a slender corolla and thick and long stigma in the floral organs; The third category gathers 6 cultivars with short stigma and average comprehensive performance of floral organ traits; The fourth type is two cultivars with variations in stigma shape. The research results can provide support for the classification of tulip cultivars and the evolution of their phylogenetic relationships from a morphological perspective, and provide reference basis for further development of excellent floral organ ornamental traits, germplasm innovation, and variety improvement of tulips.

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  • 收稿日期:2024-04-17
  • 最后修改日期:2024-06-21
  • 录用日期:2024-10-18
  • 在线发布日期: 2024-11-12
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