【Objective】Due to the shortage of arable land in China, establishing an accurate and reliable method for identifying salt tolerance in sesame seedlings is beneficial for discovering salt tolerant varieties and salt tolerant related genes, facilitating large-scale salt tolerance identification of sesame, and promoting the production process of sesame saline alkali land. 【Method】 28 sesame germplasms with significant differences in salt tolerance were used as materials. After being cultivated with clean water until the seedling stage, they grew under different concentrations of NaCl (0, 70, 140, 210, 280, and 350 mmol/L) stress. After a period of time, their plant height, fresh weight, electrical conductivity, dry weight, and other indicators were measured. Variance analysis, principal component analysis, and membership function analysis were performed on the relative indicator values, Screening suitable NaCl concentration and measurement indicators for salt tolerance identification and evaluation during sesame seedling stage. Afterwards, a 210 mmol/L NaCl solution was used as the treatment concentration, and relative fresh weight and relative plant height were used as measurement indicators to identify the salt tolerance of 239 sesame core germplasm during the seedling stage. The correlation analysis between the identification results and whether the germplasm source belongs to saline alkali land was conducted. 【Result】 Statistics and analysis of variance were conducted on the various indicators of 28 materials under different concentrations of NaCl stress. The optimal stress concentration for identifying salt tolerance in sesame seedlings was 210 mmol/L. Through principal component analysis and membership function analysis, the salt tolerance coefficients of 6 agronomic traits can be transformed into comprehensive evaluation D values of salt tolerance, mainly based on relative plant height and relative fresh weight. By using regression analysis method to establish a regression equation between the D value and the salt tolerance coefficients of seven traits: D=-0.171+1.081 * relative fresh weight+0.253 * relative plant height. Relative fresh weight value was used to classify the salt tolerance levels of sesame seedlings into five levels: high salt tolerance, salt tolerance, intermediate type, salt sensitive, and salt extremely sensitive. Five high salt tolerant varieties were screened out from 239 materials for salt tolerance identification during the seedling stage. The correlation analysis between salt tolerance identification results and germplasm sources showed that salt tolerance of sesame varieties in different geographical locations was significantly positively correlated with the degree of soil salinization in the area, providing materials for subsequent analysis of salt gene family evolution in sesame germplasm. 【Conclusion】 The optimal NaCl stress concentration for salt tolerance identification in sesame seedlings is 210mmol/L, and six indicators such as relative fresh weight can be used as suitable evaluation indicators; Five high salt tolerant varieties were identified, and 239 materials showed a significant positive correlation between salt tolerance and source.