1.晋中市农业种业繁育改良站,山西晋中030600;2.山西农业大学农学院,太原030031
研究方向为小麦品种选育和管理,E-mail:nhz1978@163.com
牛瑜琦,研究方向为小麦遗传育种,E-mail:53276014@qq.com
国家重点研发计划项目(2022YFD1200901);山西省小麦育种联合攻关(NYGG26);山西农业大学科技创新提升工程(CXGC202305);山西农业大学农学院育种工程专项(YZ2021-0)
1.Jinzhong Agricultural Seed Industry Breeding and Improvement Station,Jinzhong 030600,Shanxi;2.College of Agricultural, Shanxi Agricultural University, Taiyuan 030031
Foundation projects: National Key R&D Program of China(2022YFD1200901); Shanxi Province Wheat Breeding Joint Research(NYGG26); Shanxi Agricultural University Science and Technology Innovation Enhancement Project(CXGC202305); Breeding Engineering Project of College of Agriculture, Shanxi Agricultural University(YZ2021-0)
小麦白粉病是一种严重危害小麦生产的真菌病害,从现有资源中鉴定抗病种质并挖掘新的抗病基因是持续改良小麦抗病性的重要途径。四月黄是课题组前期从山西省小麦种质资源中鉴定出的一个抗白粉病小麦地方品种,本研究采用苗期单小种人工接种的方法对四月黄与晋麦47以及二者构建的遗传群体进行抗性鉴定和遗传分析,并采用分离群体分组分析法和90K SNP芯片扫描对四月黄携带的抗病基因进行定位。结果表明:四月黄对白粉病的抗性受一对显性核基因控制,遗传方式符合孟德尔遗传规律,暂命名为PmSYH。SNP芯片结果显示,多态性SNP标记主要富集在小麦7D染色体110~140 Mb区段和570~610 Mb区段。在上述区段分别开发SSR分子标记并利用抗、感病小群体进行连锁性筛选,结果从110~140 Mb区段筛选出6个与抗病基因连锁的SSR标记,从570~610 Mb区段未筛选出连锁标记。利用6个与PmSYH连锁的SSR标记对晋麦47/四月黄的F2群体进行基因分型,将PmSYH定位于位于7D染色体上132.6 Mb~137.5 Mb的物理区段,两侧标记分别为Sxau7DS-37和Sxau7DS-48,遗传距离分别为1.8 cM和3.1 cM。与前人定位结果比较分析表明,PmSYH是一个新的抗白粉病基因。本研究为小麦抗白粉病育种提供了新的基因资源,在北部冬麦区小麦白粉病抗性改良中具有一定的利用价值。
Powdery mildew is a devastating fungal pathogen that threatens wheat production. Identifying resistant germplasm and exploring resistance genes are crucial for sustainable improvement of wheat genetic resistance. Siyuehuang, a wheat landrace identified with resistance to powdery mildew from Shanxi Province’s wheat germplasm resources, was crossed with susceptible cultivar Jinmai 47. The segregation population was developed and subjected for artificial inoculation for resistance assessment and genetic analysis at seedling stage. We employed the bulked segregant analysis (BSA) combined with a 90K SNP array to locate the resistance genes. The resistance of Siyuehuang to powdery mildew was controlled by a single dominant nuclear gene (provisionally named PmSYH), with an inheritance pattern in accordance with Mendelian inheritance law. SNP array analysis revealed that polymorphic SNP markers were mainly enriched in the 110 Mb to 140 Mb segment on the short arm of wheat chromosome 7D. SSR (Simple Sequence Repeats) molecular markers were developed in the interval, and six markers linked to PmSYH were obtained for genotyping the F2 population. PmSYH was mapped in the physical interval of 132.6 Mb to 137.5 Mb, flanked by Sxau7DS-37 and Sxau7DS-48 with genetic distance of 1.8 cM and 3.1 cM, respectively. Comparative analysis with previous localization results suggests that PmSYH is a new resistant gene against powdery mildew. This study provided wheat new genetic resources for wheat resistance breeding, with a certain value for improving resistance to powdery mildew in the northern winter wheat region.
牛慧珍,任永康,唐朝晖,等.山西小麦地方品种四月黄抗白粉病基因的遗传分析[J].植物遗传资源学报,2025,26(2):309-318.
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