菊花CmMYB15-like基因抗旱性功能鉴定
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河南农业大学风景园林与艺术学院

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Q946;S682.1

基金项目:

中国博士后科学基金(2022M721037);国家自然科学基金青年项目(32302597);河南省自然科学基金青年项目(232300420187)


Drought Resistance Function Identification of Chrysanthemum CmMYB15-like Gene
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College of Landscape Architecture and Art,Henan Agricultural University

Fund Project:

China Postdoctoral Science Foundation (2022M721037); National Natural Science Foundation of China Youth Project(32302597); Youth Project of Natural Science Foundation of Henan Province (232300420187)

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    摘要:

    菊花(Chrysanthemum morifolium)具有重要的观赏与经济价值,干旱会降低其产量和品质,产生巨大经济损失。挖掘菊花中的抗旱基因,培育抗旱品种是解决上述问题的关键之一。MYB转录因子参与植物生长发育和响应非生物胁迫。本研究于拟南芥中异源表达菊花CmMYB15-like基因,通过观察干旱胁迫后的表型变化,测定相对含水量(Relative Water Content,RWC)、丙二醛(Malondialdehyde,MDA)、可溶性糖(Soluble Sugars,SS)、超氧化物歧化酶(Super Oxide Dismutase,SOD)活性、木质素含量等指标,探究该基因的抗旱性功能。结果显示,较野生型相比,过表达CmMYB15-like提高了干旱胁迫后植株的RWC、SS含量以及SOD酶活性,降低了MDA含量,增加了主根长度和侧根数目。此外,AtPAL2、AtPAL4、At4CL1等木质素合成相关基因上调,木质素含量高于野生型。这些结果表明,CmMYB15-like参与拟南芥干旱胁迫反应,为菊花抗旱基因资源筛选和品种培育提供一定理论依据。

    Abstract:

    Chrysanthemum (Chrysanthemum morifolium) has important ornamental and economic value. Drought reduces its yield and quality, resulting in great economic losses. Digging up drought-resistant genes in chrysanthemum and breeding drought-tolerant varieties is one of the keys to solving the above problems. MYB transcription factors are involved in plant growth and development and response to abiotic stress. This study involved the heterologous expression of the CmMYB15-like gene from chrysanthemum in Arabidopsis, followed by the observation of phenotypic changes under drought stress. The investigation of drought resistance function of this gene included measurement of relative water content (RWC), malondialdehyde (MDA) levels, soluble sugars (SS), superoxide dismutase (SOD) activity, and lignin content. The results showed that overexpression of CmMYB15-like increased RWC and SS contents and SOD activity, decreased MDA content, and increased taproot length and lateral root number after drought stress, compared with the wild type. In addition, the lignin synthesis-related genes such as AtPAL2, AtPAL4 and At4CL1 were up-regulated, and the lignin content was higher than that in the wild type. These results indicate that CmMYB15-like is involved in the drought stress response of Arabidopsis Thaliana, which provides a theoretical basis for the selection of drought-resistant gene resources and breeding of chrysanthemum cultivars.

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历史
  • 收稿日期:2024-05-20
  • 最后修改日期:2024-07-04
  • 录用日期:2024-09-19
  • 在线发布日期: 2024-09-27
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