1.河北农业大学农学院,保定 071000;2.河北农业大学生命科学学院,保定 071000
研究方向为药用植物栽培,E-mail:dgs0623@163.com
马春英,研究方向为药用植物资源培育,E-mail:bushbucker@126.com
河北省自然科学基金项目(H2023204001)
1.College of Agronomy, Hebei Agricultural University, Baoding 071000;2.College of Life Sciences, Hebei Agricultural University, Baoding 071000
Foundation project: Natural Science Foundation of Hebei (H2023204001)
为明确知母(Anemarrhena asphodeloides)种内种质的遗传背景,本研究利用聚类分析和主成分分析等方法,对160份知母种质材料进行了基于表型性状和SSR分子标记的遗传多样性分析。结果表明,21个表型性状的多样性指数变化范围为0.99~7.60,均值为1.99;变异系数的变化范围为17.22%~151.07%,均值为51.49%。采用新开发的27对SSR引物对160份知母种质材料进行毛细管电泳,共检测到143个等位基因,有效等位基因数变幅为0.56~7.40,均值为3.54;Shannon’s信息指数变幅为0.17~0.51,均值为0.36;多态信息含量变幅为0.15~0.50,均值为0.38。聚类分析表明,160份知母种质材料并没有明显的分开,但部分同一来源的知母成簇出现。主成分分析与聚类分析相互验证,也呈现相似的结果,这可能与不同地区的知母种植者频繁交换种子种苗有关。本研究通过卡方检验和T检验筛选出与6个表型性状相关联的9个SSR标记,可用于知母分子标记辅助育种。
In order to clarify the genetic background of the germplasm of Anemarrhena asphodeloides, the genetic diversity of 160 germplasm materials of A.asphodeloides was analyzed based on phenotypic traits and SSR markers using cluster analysis and principal component analysis. The results showed that the diversity index of 21 phenotypic traits ranged from 0.99 to 7.60, with an average value of 1.99. The coefficient of variation ranged from 17.22% to 151.07%, with an average of 51.49%. A total of 143 alleles were detected by capillary electrophoresis using 27 pairs of newly developed SSR primers. The effective alleles ranged from 0.56 to 7.40, with an average of 3.54. Shannon's information index ranges from 0.17 to 0.51, with an average value of 0.36. Polymorphism information content (PIC) ranged from 0.15 to 0.50, with an average of 0.38. Cluster analysis showed that there was no obvious separation of 160 germplasm materials, but some of the same source of germplasm appeared in clusters. Principal component analysis and cluster analysis verified each other and showed similar results, which may be related to the frequent exchange of seeds and seedlings by the seed growers in different regions. In this study, 9 SSR markers associated with 6 phenotypic traits were screened by Chi-square test and T-test, which could be used for marker assisted breeding of female breeding.
狄根森,闫立萱,任俊男,等.基于表型性状和