水稻核心种质耐盐鉴定与分子标记开发应用
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1.中国农业科学院作物科学研究所/作物基因资源与育种全国重点实验室;2.济宁市农业科学院,山东济宁;3.山东省水稻研究所

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基金项目:

国家重点研发项目(2021YFD1200100);中国农业科学院南繁专项“YYLH2402”


Identification of salt tolerance in rice core germplasm and development and application of molecular markers
Author:
Affiliation:

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/State Key Laboratory of Crop Gene Resources and Breeding

Fund Project:

the National Key R&D Program of China(2021YFD1200100); Nanfan Special Project of Chinese Academy of Agricultural Sciences (YYLH2402

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    摘要:

    水稻是盐敏感植物,土壤盐渍化对于水稻产量影响巨大。发掘并聚合耐盐基因的优异单倍型,创制耐盐种质,对于水稻耐盐品种选育及我国盐碱地的高效利用都具有重要意义。本实验首先对水稻3K数据库的236份核心种质进行苗期及大田耐盐鉴定,筛选出一份来自澳大利亚的强耐盐种质‘71011’,其在150mM NaCl处理条件下存活天数25.5,耐盐等级5.2;大田盐胁迫浓度为0.3%~0.5%条件下耐盐存活率100%;利用236份核心种质对已报道的、功能清晰的20个耐盐基因进行单倍型分析,筛选出AKT1、CPK12、MYB48、P5CS1、SIK1、SKC1、SNAC1、HKT1共8个与耐盐性状相关联的基因单倍型。然后利用耐盐品种‘盐丰47’与普通品种‘农垦57’作为亲本,分析序列差异,表达量验证以及构建重组自交系,最终开发出AKT1、MYB48以及HKT1这三个耐盐相关基因的分子标记,利用分子标记聚合耐盐基因优异单倍型,创制出强耐盐的新品系。研究结果为水稻耐盐育种创制了可利用的分子标记、耐盐品种资源与创新种质。

    Abstract:

    Rice is a salt sensitive plant, and soil salinization has a significant impact on rice yield. Therefore, exploring excellent haplotypes of salt tolerant genes and creating excellent salt tolerant germplasm are of great significance for rice breeding. This study firstly identified 236 core germplasm samples from the 3K rice database for salt tolerance investigation during seedling stage and whole growth period in the field. A strong salt tolerant germplasm ''71011'' from Australia was identified. This germplasm survived for 25.5 days and had a salt tolerance level of 5.2 under 150mM NaCl treatment conditions, 100% survival rate under 0.3-0.5% salt treatment in the paddy field. Using the 236 core germplasm population, haplotype analysis was conducted on 20 reported salt tolerance genes. A total of 8 genes, including AKT1, CPK12, MYB48, P5CS1, SIK1, SKC1, SNAC1, and HKT1, was identified that had haplotypes associated with salt tolerance. Then, one salt tolerant rice variety ‘Yanfeng 47’ and one normal variety ‘Nongken 57’ were selected as parents for recombinant inbred lines construction. Finally, three molecular markers were designed for elite haplotypes of three genes, AKT1, MYB48, and HKT1. These molecular markers were confirmed by PCR and qRT-PCR. We innovated three high salt tolerance lines by aggregating elite haplotypes of salt tolerant genes by molecular marker assistance breeding. Our results provided available germplasm resources, molecular markers and innovative lines for breeding new salt-tolerant rice varieties.

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历史
  • 收稿日期:2024-06-02
  • 最后修改日期:2024-07-19
  • 录用日期:2024-08-07
  • 在线发布日期: 2024-08-23
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