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首页 > 过刊浏览>2025年第26卷第3期 >470-480. DOI:10.13430/j.cnki.jpgr.20240602001
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水稻核心种质耐盐鉴定与分子标记开发应用
作者:
作者单位:

1.中国农业科学院作物科学研究所/作物基因资源与育种全国重点实验室，北京 100081;2.三亚中国农业科学院国家南繁研究院， 海南三亚 572024;3.济宁市农业科学院，山东济宁 272007;4.山东省水稻研究所，济南 250100

作者简介:

研究方向为种质资源创新利用，E-mail： wangshizhuang2021@163.com；聂亚敏为共同第一作者

通讯作者:

乔卫华，研究方向为种质资源创新利用，E-mail： qiaoweihua@caas.cn
杨庆文，研究方向为种质资源创新利用，E-mail： yangqingwen@caas.cn
王文生，研究方向为种质资源创新利用，E-mail： wangwensheng02@caas.cn

基金项目:

国家重点研发项目（2021YFD1200100）；中国农业科学院南繁专项（YYLH2402）


Identification of Salt Tolerance in Rice Core Germplasm and Development and Application of Molecular Markers
Author:
Affiliation:

1.Institute of Crop Sciences， Chinese Academy of Agricultural Sciences/ State Key Laboratory of Crop Gene Resources and Breeding， Beijing 100081;2.National Nanfan Research Institute (Sanya)， Chinese Academy of Agricultural Sciences， Sanya 572024， Hainan;3.Jining Academy of Agricultural Sciences， Jining 272007，Shandong;4.Rice Research Institute， Shandong Academy of Agricultural Sciences， Jinan 250100

Fund Project:

Foundation projects： National Key R&D Program of China（2021YFD1200100）； Nanfan Special Project of Chinese Academy of Agricultural Sciences （YYLH2402）

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    摘要:

    水稻是盐敏感植物，土壤盐渍化对水稻产量影响巨大。发掘并聚合耐盐基因的优异单倍型，创制耐盐种质，对于水稻耐盐品种选育及我国盐碱地的高效利用都具有重要意义。本研究首先对水稻3K数据库的236份核心种质进行苗期及大田耐盐鉴定，筛选出一份来自澳大利亚的强耐盐种质‘71011’，其在150 mmol/L NaCl处理条件下存活天数25.5 d，耐盐等级5.2， 大田盐胁迫浓度为0.3%~0.5%条件下耐盐存活率100%；利用236份核心种质对已报道的、功能清晰的20个耐盐基因进行单倍型分析，筛选出AKT1、CPK12、MYB48、P5CS1、SIK1、SKC1、SNAC1、HKT1共8个与耐盐性状相关联的基因单倍型。然后利用耐盐品种‘盐丰47’与普通品种‘农垦57’作为亲本，分析序列差异、验证表达量并构建重组自交系，最终开发出AKT1、MYB48以及HKT1三个耐盐相关基因的分子标记，并利用分子标记聚合耐盐基因优异单倍型，创制出强耐盐的新品系。研究结果为水稻耐盐育种提供了可利用的分子标记、耐盐品种资源与创新种质。

    Abstract:

    Rice is a salt-sensitive plant， and soil salinization significantly impacts rice performance. Therefore， exploring excellent haplotypes of salt-tolerant genes and generating elite germplasm are of great significance for rice breeding. In this study， we firstly analyzed 236 core germplasm lines collected from the 3K Rice Genome Project Database， for salt tolerance during the seedling stage and throughout the entire growth period in the field. A highly salt-tolerant germplasm ‘71011’ from Australia was identified. This germplasm survived for 25.5 d and exhibited a salt tolerance level of 5.2 under 150 mmol/L NaCl treatment conditions， with a 100% survival rate under 0.3% to 0.5% salt treatment in the paddy field. We further conducted haplotype analysis on 20 reported salt tolerance genes in the core collection. Eight genes， including AKT1， CPK12， MYB48， P5CS1， SIK1， SKC1， SNAC1， and HKT1， were identified as having haplotypes associated with salt tolerance. One salt-tolerant variety ‘Yanfeng 47’ and one normal variety ‘Nongken 57’ were selected as parents to generate recombinant inbred lines. Finally， three molecular markers， which have been confirmed by PCR and qRT-PCR， were designed for selecting elite haplotypes of three genes， AKT1， MYB48， and HKT1. Through marker-assisted selection， we successfully developed three high salt-tolerance lines by aggregating these elite haplotypes. Our results provide available germplasm resources， molecular markers and innovative lines for breeding new salt-tolerant rice varieties.

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王世壮,聂亚敏,黄婧芬,等.水稻核心种质耐盐鉴定与分子标记开发应用[J].植物遗传资源学报,2025,26(3):470-480.

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  • 收稿日期:2024-06-02
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