图像识别技术在杏品种资源叶片形态评估与多样性研究中的应用
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辽宁省果树科学研究所

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国家自然科学基金项目(面上项目,重点项目,重大项目)


Application of image recognition technology in the assessment of leaf morphology and diversity of apricot germplasm
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The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)

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    摘要:

    摘要:为了探讨杏叶片形态的遗传多样性,方便杏种质资源收集和品种识别。本研究以142份杏种质资源为材料,对杏叶片的28个表型性状进行了调查。结果表明,28个表型性状中13个分级性状和8个数量性状变异系数高于10%。表明142份杏品种资源的叶片表型存在丰富的遗传多样性。相关性分析,发现叶片面积、叶片周长、叶片长、叶片宽4个性状间存在极显著的正相关;叶基形状、叶基角度与叶片长宽比呈极显著负相关;叶片圆度与叶尖长短呈显著负相关。主成分分析,结果显示第1主成分由叶片大小相关性状组成;第2主成分为叶片颜色相关性状;第3和4主成分分别反映了叶片形状与叶片锯齿高度。根据聚类分析将杏种质资源大致划分为小叶-深绿类、大叶-截形类、小叶-圆形类、中叶-浅绿类、大叶-长叶柄类、中叶-楔形类和中叶-圆形类等8个类群和6个亚群。本研究基于图片识别技术能够对杏品种资源叶片形态特征进行快速评价,增加叶尖长短、叶缘锯齿深浅,叶片最宽处位置3个叶片分级性状可以作为识别不同杏品种的依据,对于今后我国杏种质资源的叶片形态评价与品种识别具有一定的参考价值。

    Abstract:

    Abstract: In order to explore the genetic diversity of apricot leaf morphology, and to facilitate the collection of apricot germplasm resources and variety identification. In this study, the comparative analyses were carried out by using various methods, such as descriptive statistics, principal component analysis and cluster analysis. The results showed the coefficients of variation (cv) of the 13 graded traits were all above 20%, and the three leaf traits of leaf tip length, leaf margin serration depth, and position of the widest part of the leaf blade differed greatly, which can be used as traits to distinguish leaf morphology of different apricot varieties. The magnitude of variation for the 15 quantitative traits ranged from 4.16% to 29.5%, which showed that the degree of leaf variation among apricot varieties was high, and there were a variety of types and rich genetic diversity among apricot varietal resources. Through correlation analysis, it was found that there was a highly significant positive correlation between four traits: leaf blade roundness, leaf blade rectangularity, leaf apex angle and leaf base angle; leaf base shape was highly significantly and positively correlated with leaf base angle with a correlation coefficient of 0.92, and highly significant negatively correlated with leaf length-to-width ratio; and leaf roundness was significantly negatively correlated with leaf tip length, which indicated that tip length of leaf to a certain extent could reflect the leaf shape. Through principal component analysis, the 15 quantitative traits were divided into four composite factors, and the cumulative contribution rate reached more than 85.04%. The results showed that the first principal component consisted of five traits, including leaf area, leaf circumference, leaf length, leaf width, and petiole length, which mainly reflected the traits related to the size of the leaf blade of the apricot germplasm resources; the second principal component reflected the traits related to the color of the leaf blade; and the third and the fourth principal components reflected the traits related to leaf blade shape and leaf blade serration ratio, respectively. The four dominant factors of leaf size, leaf color, leaf shape and average serration height can be used as the main phenotypic indexes for classification of apricot germplasm resources. Apricot germplasm resources were classified into 8 taxa based on cluster analysis. Taxon Ⅰ consisted of only 2 germplasm, Zao Dahuang and Chaoxianxing, which were in the large-leaved-cuneate category; Taxon Ⅱ and Ⅲ both consisted of 1 material, which was Huanna and Zhuolumuguxing, respectively; Taxon Ⅳ consisted of 18 germplasm, which were in the small-leaved-deep-green category; Taxon Ⅴ consisted of 1 Central Asian germplasm, which was in the Stella category; Taxon Ⅵ consisted of 1 material, which was in the large-leaved-cardinal category; Taxon VII consisted of 12 germplasm, which were in the large-leaved-truncate category; Taxon VIII was further classified into 6 subgroups. Taxon VII consists of 12 germplasm, which is large-leaved-truncate; Taxon VIII is further divided into six subgroups. In this study, based on the application of image recognition technology, we were able to rapidly evaluate the leaf morphology of apricot varieties and resources, and increase the length of the leaf tip, the depth of the leaf margin serration, and the position of the widest part of the leaf . These three leaf grading traits can be used as the basis for identifying different apricot varieties, which will be of certain reference value for the future evaluation of leaf morphology of China's apricot germplasm resources and varietal identification.

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  • 收稿日期:2024-06-16
  • 最后修改日期:2024-07-19
  • 录用日期:2024-11-12
  • 在线发布日期: 2024-11-15
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