1.江西省林业科学院樟树研究所/国家林业草原樟树工程技术研究中心,南昌330032;2.江西省林业科学院樟树研究所/ 江西乡土树种良种选育与高效利用江西省重点实验室,南昌 330032
研究方向为林木种质资源挖掘与创新,E-mail : zhengyongjie0416@163.com
伍艳芳,研究方向为林木遗传育种,E-mail : yanfangwu2012@163.com
江西省林业科技创新项目[(2021)15号];江西省林业科学院基础研究与人才培养专项(2023522704)
ZHENG Yongjie
Camphor Tree Research Institute, Jiangxi Academy of Forestry/Camphor Engineering and Technology Research Center of National Forestry and Grassland Administration, Nanchang 330032;Camphor Tree Research Institute, Jiangxi Academy of Forestry/Jiangxi Provincial Key Laboratory for Breeding and Efficient Utilization of Jiangxi Native Tree Species, Nanchang 330032ZHANG Yueting
Camphor Tree Research Institute, Jiangxi Academy of Forestry/Camphor Engineering and Technology Research Center of National Forestry and Grassland Administration, Nanchang 330032;Camphor Tree Research Institute, Jiangxi Academy of Forestry/Jiangxi Provincial Key Laboratory for Breeding and Efficient Utilization of Jiangxi Native Tree Species, Nanchang 330032LIU Xinliang
Camphor Tree Research Institute, Jiangxi Academy of Forestry/Camphor Engineering and Technology Research Center of National Forestry and Grassland Administration, Nanchang 330032;Camphor Tree Research Institute, Jiangxi Academy of Forestry/Jiangxi Provincial Key Laboratory for Breeding and Efficient Utilization of Jiangxi Native Tree Species, Nanchang 330032Wu Yanfang
Camphor Tree Research Institute, Jiangxi Academy of Forestry/Camphor Engineering and Technology Research Center of National Forestry and Grassland Administration, Nanchang 330032;Camphor Tree Research Institute, Jiangxi Academy of Forestry/Jiangxi Provincial Key Laboratory for Breeding and Efficient Utilization of Jiangxi Native Tree Species, Nanchang 3300321.Camphor Tree Research Institute, Jiangxi Academy of Forestry/Camphor Engineering and Technology Research Center of National Forestry and Grassland Administration, Nanchang 330032;2.Camphor Tree Research Institute, Jiangxi Academy of Forestry/Jiangxi Provincial Key Laboratory for Breeding and Efficient Utilization of Jiangxi Native Tree Species, Nanchang 330032
Foundation projects: Innovation Fund of Jiangxi Province Department of Forestry Research [(2021)No.15]; Fundamental Research and Talent Development Project of Jiangxi Academy of Forestry (2023522704)
浙江楠是我国珍稀濒危物种,研究浙江楠群体的遗传多样性和遗传结构,可为有效保护和利用浙江楠种质资源提供科学依据。本研究采用自主开发的15对SSR引物,对浙江楠10个群体共计175份种质资源进行毛细管电泳检测,解析浙江楠群体的遗传多样性和遗传结构。15对引物在175份浙江楠种质中共检测到113个等位基因位点(Na),平均每对引物含2.371个有效等位基因(Ne),多态引物信息含量(PIC)为0.323~0.844,平均值为0.629。浙江楠群体表现出中等程度的遗传多样性(平均观测杂合度=0.621,平均期望杂合度=0.478),平均群体内近交系数(Fis)为-0.237,平均群体近交系数(Fit)为0.082,杂合率较高,群体间表现出较高的遗传分化系数(Fst=0.275)和低水平的平均基因流(Nm=0.763)。分子方差分析表明浙江楠种群内个体间差异是主要的遗传变异来源,占总变异的78.7%(P<0.001)。群体结构分析与聚类分析将10个不同种源的浙江楠群体分为3个类群,群体间存在一定的基因渐渗。生境破碎化和人为干扰可能是造成浙江楠濒危的主要原因,应采取就地保护和迁地保护相结合的措施,保护浙江楠的遗传多样性。
Phoebe chekiangensis is a rare and endangered species in China, and investigating its genetic diversity and genetic structure of populations provides a scientific basis for the effective protection and utilization. In this study, 15 pairs of SSR markers were used to analyze 175 germplasm resources from 10 populations by capillary electrophoresis. A total of 114 alleles (Na) were detected, with an average of 2.371 effective alleles (Ne) per primer pair. The polymorphic information content (PIC) of the SSR primers ranged from 0.323 to 0.844, averaging 0.629. The populations exhibited a moderate degree of genetic diversity (Ho=0.621, He=0.478). The inbreeding coefficient within subpopulations (Fis) was -0.237, while the inbreeding coefficient for total population (Fit) was 0.082, indicating a high rate of heterozygosity. There was substantial genetic differentiation (Fst=0.275) and limited gene flow (Nm=0.763) among populations. Analysis of molecular variance (AMOVA) showed that inter-individual differences within populations accounted for 78.7% of the total variation (P<0.001). Population structure and cluster analysis divided the 10 populations from different provenances into three groups, with some evidence of gene introgression between populations. Habitat fragmentation and human disturbance are likely the primary reasons of endangerment for P. chekiangensis. To protect its genetic diversity of P. chekiangensis, measures by combining in situ conservation and ex situ conservation should be implemented.
郑永杰,张月婷,刘新亮,等.珍稀濒危植物浙江楠的遗传多样性评价[J].植物遗传资源学报,2025,26(3):566-574.
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