江苏省林业科学研究院工程中心,南京 211153
主要从事林木花卉繁育技术研究,Email: xiaojingzi1229@163.com
张 敏,主要从事生物技术与林木花卉良种繁育工作,Email: 29157510@qq.com
中央财政林业科技推广示范资金项目(苏[2024]TG08);江苏省林业科学研究院自主科研项目(ZZKY202303);江苏省林业科学研究院青年科技基金项目(JAF-2016-07)
Engineering Center,Jiangsu Academy of Forestry,Nanjing 211153
Foundation projects: Central Finance Forestry Science and Technology Promotion Demonstration Fund Project(Su[2024]TG08); Independent Research Projects of Jiangsu Academy of Forestry(ZZKY202303);Jiangsu Academy of Forestry Youth Foundation(JAF-2016-07)
为更好地保护、开发和利用乌饭树资源,采用基因分型测序(GBS,genotyping by sequencing)技术对采集于5个地区的70份乌饭树野生种质进行了SNP分子标记开发,利用开发的标记研究了乌饭树种质的遗传多样性和群体遗传结构。结果显示,通过测序数据分析共筛选得到9752个高质量SNP标记用于后续研究;乌饭树5个群体均具有丰富的遗传多样性,平均有效等位基因数为1.523,平均期望杂合度为0.219,平均观测杂合度为0.231,其中江苏溧阳群体的遗传多样性最高。分子方差分析显示整个群体的遗传变异主要来自个体间(65.45%)。群体间遗传分化系数显示5个群体间遗传分化程度高,其中江苏溧阳与江西上犹群体间的遗传分化系数最高,为0.406。系统进化树(邻接法)显示乌饭树种质聚类为3个进化分支,种质的聚集与其地理来源无确定性关系;群体结构分析将乌饭树种质分为3个亚群,主成分分析结果与群体结构的结果类似,不同地区的乌饭树种质在3个亚群中均有分布,显示了乌饭树资源存在着广泛的基因交流。本研究可为今后乌饭树良种选育和资源保护提供参考。
In order to provide reference for the protect, development and utilization of Vaccinium bracteatum resources, a total of 70 germplasm resources from 5 geographical populations were genotyped by GBS (Genotyping-by-Sequencing) technique to develop SNP markers. Using the developed markers, we investigated the genetic diversity and population genetic structure of V. bracteatum. The result shows a total of 9752 highly consistent and effective SNP markers were obtained through sequencing data analysis. 5 geographical populations of V. bracteatum all have high levels of genetic diversity, with an average effective number of alleles (Ne) of 1.523, expected heterozygosity (He) of 0.219, and observed heterozygosity (Ho) of 0.231. Among the 5 populations, the Liyang population had the highest genetic diversity. Analysis of variance for molecular variation(AMOVA) revealed that 64.45% of the total genetic variation resided among individuals. The fixation index (Fst) showed a high degree of genetic differentiation among the 5 geographical populations, with the highest Fst values(0.406) observed between the Liyang and Shangyou populations. Based on the results of NJ Phylogenetic tree, V. bracteatum resources clustered into three branches, which did not show obvious correspondence between geographical location and genetic relationships. The STRUCTURE analysis clustered the V. bracteatum resources into three subgroups, the results of principal component analysis was consistent with it, and different geographical populations are distributed in each subgroup, indicating the existence of high gene exchange in V. bracteatum resources. This study provides reference for selection and breeding of V. bracteatum and germplasm conservation.
黄婧,张敏.基于