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首页 > 过刊浏览>2025年第26卷第3期 >507-518. DOI:10.13430/j.cnki.jpgr.20240621001
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高粱BZR基因的鉴定及与籽粒性状关联分析
作者:
作者单位:

1.山西农业大学高粱研究所，榆次 030600;2.山西农业大学农学院，太谷 030801;3.山西农业大学社会服务部，太原 030031

作者简介:

研究方向为高粱遗传育种，E-mail ： zjy0502@yeah.net

通讯作者:

张一中，研究方向为高粱遗传育种，E-mail ： zhyzh225@163.com

基金项目:

国家自然科学基金（32241042）；山西省博士毕业生、博士后研究人员来晋工作奖励经费科研项目（SXBYKY2023022）；山西省科技重大专项计划“揭榜挂帅”项目（202101140601027）；山西农业大学高粱研究所国家基金培育项目（GLS-gp-202402）


Identification of BZR Genes and Its Association with Grain Traits in Sorghum
Author:
Affiliation:

1.Sorghum Research Institute， Shanxi Agricultural University， Yuci 030600;2.College of Agriculture， Shanxi Agricultural University， Taigu 030801;3.Social Service Department， Shanxi Agricultural University， Taiyuan 030031

Fund Project:

Foundation projects： National Natural Science Foundation of China （32241042）； The Scientific Research Project of Shanxi Province Outstanding Doctoral and Postdoctoral Researchers Work Award Fund （SXBYKY2023022）； The Major Special Science and Technology Projects in Shanxi Province （202101140601027）； National Fund Cultivation Project of Sorghum Research Institute， Shanxi Agricultural University （GLS-gp-202402）

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    摘要:

    油菜素内酯（BRs，brassinosteroids）是植物体内存在的一种重要激素，在调控植物生长发育及抵抗多种环境胁迫过程中发挥作用。BZRs转录因子是BRs信号途径中的关键调节因子，通过调控靶基因的表达传递BRs信号。为了深入研究高粱BZRs基因的生物学特性及功能，本研究从高粱基因组中鉴定获得10个BZR基因。组织特异性表达分析显示，SbBZR3、SbBZR4和SbBZR10在多个组织中高表达，且均在籽粒发育过程中的表达量较高。同时，基于340份高粱种质资源的重测序结果及籽粒性状表型数据，鉴定SbBZRs的优异等位变异，结果表明，SbBZR3的优异等位变异SbBZR3-SNP404（T）与粒长显著相关，SbBZR10的优异等位变异SbBZR10-SNP660（A）与粒长、粒宽和千粒重显著相关，且在进化过程中SbBZR3-SNP404（T）和SbBZR10-SNP660（A）均受到了人工选择。本研究不仅为揭示高粱BZRs基因的作用机制奠定了基础，也将为利用优异等位基因改良籽粒大小、促进高产高粱新种质的创制提供参考。

    Abstract:

    Brassinosteroids （BRs） are class of important hormones in plants， which play a role in regulating plant growth and development and resisting various environmental stresses. BZR transcription factors are key regulatory factors in the BRs signaling pathway and transmit BRs signals by regulating the expression of target genes. To further study the biological characteristics and functions of BZR genes in sorghum， this study identified 10 BZR genes from the sorghum genome. The tissue-specific expression analysis showed that SbBZR3， SbBZR4， and SbBZR10 are highly expressed in multiple tissues as well as have high expression levels during the grain development process. Meanwhile， based on the resequencing results and grain phenotype data of 340 sorghum germplasm resources， the superior alleles of SbBZRs were identified. The results showed that the superior alleles SbBZR3-SNP404 （T） was significantly associated with grain length， and SbBZR10-SNP660 （A） was significantly associated with grain length， grain width and 1000-grain weight. Both SbBZR3-SNP404 （T） and SbBZR10-SNP660 （A） were artificially selected during the evolutionary process. This study not only lays a foundation for revealing the action mechanism of BZR genes in sorghum， but also provides a reference for using excellent alleles to improve grain size and promote the creation of new sorghum seeds with high yield.

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赵娟莹,李金彪,李皓翔,等.高粱BZR基因的鉴定及与籽粒性状关联分析[J].植物遗传资源学报,2025,26(3):507-518.

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  • 收稿日期:2024-06-21
  • 在线发布日期: 2025-03-07
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