OsELF3-1与OsARID3互作调控水稻根部性状
作者:
作者单位:

1.沈阳农业大学水稻所;2.&3.lt;4.sup&5.gt;6.沈阳农业大学水稻所&7./sup&8.辽东学院农学院

作者简介:

通讯作者:

中图分类号:

基金项目:

沈阳市科学技术局种业创新专项(23-410-2-02—01)


OsELF3-1 interacts with OsARID3 to regulate root traits in rice
Author:
Affiliation:

Fund Project:

Shenyang Science and Technology Bureau seed industry innovation project (23-410-2-02—01)

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    根系是水稻植株的重要组成部分,在植物固定、水份和营养物质获取等生长发育过程中起重要作用。本研究发现oself3-1突变体的根系显著变短,将突变体与野生型杂交构建F2分离群体,进行遗传分析表明突变性状由隐性单基因调控。通过图位克隆将OsELF3-1定位在6号染色体一段50.9 kb的区间内,该区间内共有4个开放阅读框(ORF),通过序列比对发现突变体在ORF4(OsELF3-1)的第二个外显子上缺失7个碱基,导致基因发生移码并提前终止,推测OsELF3-1为目标基因。调查OsELF3-1的CRISPR/Cas9敲除突变体发现其根系显著短于野生型,验证OsELF3-1参与根系长度调控。为进一步阐明OsELF3-1的调控网络,利用酵母双杂交筛选到OsELF3-1的互作蛋白OsARID3,OsARID3具有ARID功能结构域、α-crystallin/Hsp_domain、SHSP结构域,以及依靠钾离子的钠离子/钙离子交换的结构域。调查OsARID3-RNAi突变体发现其根系显著短于野生型,说明OsARID3可能参与根系长度调控。通过3 K测序数据分析OsARID3自然变异发现其共有15个单倍型,且存在明显的籼粳分化,粳稻主要属于单倍型I、IV和VI。综上所述,本研究发现OsELF3-1与OsARID3互作调控水稻根系,为进一步深入解析水稻根系生长发育调控网络提供理论基础和种质资源。

    Abstract:

    The root system is an important part of rice plant and plays an important role in plant fixation, water and nutrient acquisition. In this study, it was found that the root system of oself3-1 mutant became significantly shorter, the mutant was crossed with wild type to construct F2 isolated population, and genetic analysis showed that the mutant trait was regulated by recessive single gene. By mapping cloning, OsELF3-1 was located in a 50.9 kb interval of chromosome 6, in which there were four open reading frames (ORFs), sequence comparison revealed that the mutant had 7 bases missing on the second exon of ORF4 (OsELF3-1), causes the gene to frameshift and terminate prematurely, and OsELF3-1 was presumed to be the target gene. The root system of CRISPR/Cas9 mutant with OsELF3-1 was significantly shorter than that of wild type, which verified that OsELF3-1 was involved in regulating root length. In order to further clarify the regulatory network of OsELF3-1, the interacting protein OsARID3 of OsELF3-1 was screened by yeast two-hybridization, OsARID3 has an ARID functional domain, an α-crystallin/Hsp_domain, a SHSP domain, and a potassium dependent sodium/calcium ion exchange domain. Investigation of OsARID3-RNAi mutants showed that their roots were significantly shorter than those of the wild type, OsARID3 may be involved in the regulation of root length. Natural variation of OsARID3 was analyzed by 3 K sequencing data and 15 haplotypes were found, and there was obvious differentiation between indica and japonica, japonica rice mainly belonged to haplotypes I, IV and VI. In summary, this study found that OsELF3-1 and OsARID3 interact to regulate rice roots, providing theoretical basis and germplasm resources for further analysis of rice root growth and development regulation network.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2024-06-28
  • 最后修改日期:2024-07-29
  • 录用日期:2024-08-08
  • 在线发布日期: 2024-08-08
  • 出版日期:
您是第位访问者
ICP:京ICP备09069690号-23
京ICP备09069690号-23
植物遗传资源学报 ® 2024 版权所有
技术支持:北京勤云科技发展有限公司