广西壮族自治区农业科学院生物技术研究所, 南宁 530007
研究方向为特色药用植物种质资源开发与遗传改良,E-mail:shiyuhuang@gxaas.net
张尚文,研究方向为特色药用植物种质资源开发与遗传改良,E-mail:63838114@qq.com
财政部和农业农村部国家现代农业产业技术体系(CARS-21);广西重点研发计划(桂科AB21220042);广西特色作物试验站(桂TS2022002)
Biotechnology Research Institute,Guangxi Academy of Agricultural Sciences, Nanning 530007
Foundation projects: China Modern Agricultural Research System of the Ministry of Finance and the Ministry of Agriculture and Rural Affairs (CARS-21);The Key Research and Development Project in Guangxi(Guike AB21220042);Guangxi Characteristic Crop Experimental Station Project (Gui TS2022002)
通过变异系数、相关性分析、主成分分析和聚类分析等方法,对142份赤苍藤种质进行表型多样性分析及综合评价,为广西赤苍藤的品种选育奠定基础。调查发现崇左市、百色市具有较多的赤苍藤野生种质资源,其次为南宁市,主要分布在石灰岩石山、山坡、山地、疏林、密林等环境,海拔在140~1001 m之间。表型统计结果显示,142份赤苍藤种质的11个表型性状变异系数介于2.76%~18.75%,说明广西赤苍藤种质资源遗传差异较大。相关性分析表明,叶长与叶宽、第二级枝条节间长、果实纵径、叶厚有显著相关性;叶宽与叶厚、果实纵径有显著相关性;叶柄长与第二级枝条粗、第二级枝条节间长有显著相关性;海拔高度与叶厚呈显著正相关,而与叶长、叶宽、第二级枝条节间长呈显著负相关。聚类分析结果显示在欧氏距离为4.5时,142份赤苍藤种质材料可分为4类,第Ⅰ、Ⅱ、Ⅲ和Ⅳ类分别包含49份、54份、27份和12份种质材料,第Ⅰ、第Ⅱ类种质主要代表了广西地区大部分的野生种质,多数人工栽培种质被划为第Ⅲ类,该类种质在产量方面可能具有优势,第Ⅳ类种质主要来源于海拔较高地区,可能在抗寒方面表现更优。主成分分析表明,5个主成分的累计贡献率达68.25%,主成分1和主成分2主要反映叶片和枝条相关指标,而主成分3、主成分4和主成分5主要反映果实相关指标。综合评价排名前10的种质均来源于崇左市,其中包含5份人工栽培种质和5份野生种质,可为赤苍藤新品种选育及品种改良提供优良亲本。
The phenotypic diversity analysis and comprehensive evaluation of 142 germplasms of Erythropalum scandens were carried out by means of coefficient of variation, correlation analysis, principal component analysis and cluster analysis, which laid a foundation for the breeding of E. scandens in Guangxi. The survey found that Chongzuo City and Baise City had more wild germplasm resources, followed by Nanning City. The wild germplasm mainly distributed in limestone hills, slopes, mountains, sparse forests, dense forests, with an altitude of 140-1001 m. The statistical results showed that the coefficient of variation of 11 phenotypic traits was between 2.76% and 18.75% of 142 germplasms, indicating that the genetic difference of germplasm resources in Guangxi was large. Correlation analysis showed that leaf length was significantly correlated with leaf width, length of the second branch internode, longitudinal diameter of fruit and leaf thickness. Leaf width was significantly correlated with leaf thickness and longitudinal diameter of fruit. Leaf stalk length was significantly correlated with diameter of the second branch, length of the second branch internode. Altitude was significantly positively correlated with leaf thickness, but significantly negatively correlated with leaf length, leaf width and length of the second branch internode. The results of cluster analysis showed that 142 germplasm materials could be divided into 4 categories when the Euclidean distance was 4.5, The I, II, III and IV categories contained 49, 54, 27 and 12 germplasms, respectively. Categories I and II represent the majority of wild germplasms from the Guangxi region. Most artificial cultivation germplasms are classified into Category III, which may have advantages in terms of yield. Category IV germplasms mainly originate from higher altitude areas and may exhibit better cold resistance. Principal component analysis showed that the cumulative contribution rate of the five principal components was 68.25%. Principal component 1 and principal component 2 mainly reflected leaf and branch related indicators, while principal component 3, principal component 4 and principal component 5 mainly reflected fruit related indicators. The comprehensive evaluation results showed that the top ten germplasms were all from Chongzuo City, including 5 cultivated germplasms and 5 wild germplasms, which could provide excellent parents for the breeding and improvement of new varieties of E. scandens.
黄诗宇,张向军,杨天为,等.广西赤苍藤种质资源调查与表型多样性评价[J].植物遗传资源学报,2025,26(4):747-760.
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