The main agronomic traits and disease resistance identification of rice germplasm resources collected in the third general survey and collection action of crop germplasm resources in Hunan Province ( hereinafter referred to as the third general survey of Hunan Province ) were evaluated. The results showed that the rice germplasm resources collected in the third general survey of Hunan Province were divided into indica subspecies and japonica subspecies, mainly indica subspecies. The proportions of sticky rice and waxy rice were close, which were 53.8 % and 46.2 %, respectively. There are 41 germplasm resources of red rice, black rice, purple rice, green rice and brown rice. The rice germplasm resources collected in the third general survey of Hunan Province are rich in genetic variation, most of which are lodging resistance, moderate stem angle, erect flag leaf, yellow glume tip, yellow glume, no awn, and most of the grain shapes are medium-long. Fourteen excellent germplasm resources with suitable growth period and plant height, high seed setting rate and strong disease resistance, large panicle and more grains and suitable 1000-grain weight were identified. At the same time, 13 excellent waxy rice germplasm resources and 20 colored functional rice germplasm resources were screened. The above excellent rice germplasm resources will provide an important material basis for basic research and new variety breeding.