The main agronomic traits and disease resistance of 225 rice germplasm resources collected during 'The Third General Survey and Collection Action of Crop Germplasm Resources in Hunan Province' project （hereinafter referred to as ‘Hunan Province Third Census’） were identified and evaluated. This collection predominantly comprised indica subspecies accounting for 94.2%， and exhibited nearly equal proportions of sticky rice （53.8%） and waxy rice （46.2%） accessions. The remarkable diversity on seed color， including white， black， red， purple， green and brown， with 41 accessions exhibiting pigmented rice traits， were observed. Their genetic variations are abundant， with coefficients of variation ranging from 6.47% to 52.54%. Most accessions showed favorable agronomic characteristics， including lodging resistance， moderate stem angle， erect flag leaf， yellow glume tip， yellow glume color， awnless and medium-long grain shape. Fourteen accessions were identified with optimal growth duration ranging from 110 to 130 days， suitable plant height （110 to 140 cm）， high seed setting rate （>90%）， strong disease resistance （medium resistance or higher）， large panicle size （≥200 grains per panicle）， and favorable 1000-grain weight （≥30 g）. Meanwhile， 13 and 20 accessions showing waxy and pigmented coloration， respectively， were identified. These germplasm resources serve as raw materials supporting the academic research and breeding for new varieties in rice.