河南农业大学农学院
国家自然科学基金项目(32201849);河南省科技攻关(232102111109)
College of Agronomy,Henan Agricultural University,Zhengzhou
The National Natural Science Foundation of China (32201849);Henan Major Science and Technology Projects (232102111109) of China
小麦籽粒色泽性状亮度(L*)、红度(a*)和黄度(b*)是小麦品质的重要指标。为挖掘调控小麦籽粒色泽相关性状的遗传位点,对黄淮麦区243份小麦材料组成的自然群体进行了4个环境下籽粒色泽性状表型调查,群体基因型信息由小麦 660K SNP芯片表征,并在此基础上进行了全基因组关联分析。结果表明,与籽粒色泽关联的显著SNP 位点共有785个,可解释11.4%~23.4% 的表型变异,其中与L*关联的SNP 位点主要分布在1A 和2B染色体上,与a*关联的SNP 位点主要分布在2A、2B、2D、4B、5B、5D、7A 和7D染色体上,与b*关联的SNP 位点主要分布在2B、5B、5D、7A、7B和7D染色体上。51个显著SNP 位点为一因多效位点。通过基因功能注释挖掘到了36个和籽粒色泽相关的候选基因,其中仅8 个基因在籽粒中表达,包括已知的 Pinb 基因。进一步通过基因多态性分析发现仅有Pinb 基因和UDP-葡萄糖/GDP-甘露糖脱氢酶基因TraesCS5B02G399800与目标性状显著关联。单倍型分析挖掘到位于TraesCS5B02G399800基因内部的差异单倍型与b*显著关联。本研究挖掘到的候选基因对小麦籽粒色泽的分子辅助选择和全基因组预测提供了参考。
Wheat kernel color related traits L*, a*, and b* are important indicators of wheat quality. In order to reveal the genetic loci that regulate wheat kernel color related traits, we investigated phenotypes of L*, a*, and b* in a natural population that composed of 243 wheat accessions in Huanghuai wheat region in four environments, and characterized the population by the wheat 660K SNP assay. Furtherly, genome-wide association studies (GWAS) was performed for wheat kernel color related traits. Results indicated that, 785 significant SNP associated with kernel color traits were identified, explaining 11.4%~23.4% phenotypic variation. SNP associated with L* are mainly distributed on chromosome 1A and 2B; SNP associated with a* are mainly distributed on chromosome 2A, 2B, 2D, 4B, 5B, 5D, 7A, and 7D; SNP associated with b* are mainly distributed on chromosome 2B, 5B, 5D, 7A, 7B, and 7D. Of them, 51 SNP were pleiotropic. Further, a total of 36 potential candidate genes related to the kernel color traits were identified by gene annotation. Only 8 of the 36 potential candidate genes are expressed in the kernel, including the known gene Pinb. Furtherly, gene polymorphism analysis showed that Pinb and UDP-glucose/GDP-mannose dehydrogenase gene TraesCS5B02G399800 were significantly associated with the target traits. Differential haplotypes of TraesCS5B02G399800 were detected significantly associated with b* by haplotype analysis. Therefore, this study provided a reference for marker-assisted selection and whole genomic prediction for wheat kernel color traits.