高丹草营养品质性状的QTL定位及候选基因筛选
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内蒙古农业大学

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国家自然科学基金项目(面上项目,重点项目,重大项目)


QTL Mapping and Screening of Candidate Gene for Nutritional Quality Traits in Sorghum Sudangrass Hybrid
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Inner Mongolia Agricultural University

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The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)

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    摘要:

    品质性状是衡量牧草优劣的重要指标,在牧草的定向改良和品种选育有着举足轻重的地位。优质牧草可有效改善牲畜饲喂效率,促进畜牧业的可持续发展。本研究以150个高丹草F2群体及其亲本为材料,在4个不同环境情况下,基于前期构建的高丹草超高密度遗传连锁图谱,采用区间作图法对其P、WSC、NDF、ADF和K五个品质性状做QTL定位分析,并在置信区间内筛选与其相关的候选基因。结果显示,以LOD≥3为阈值,共定位到198个QTLs,其中,有16个QTLs在多个环境下被重复检测到,主要分布在LG3、4、6、7和8连锁群上,单个贡献率为10% ~ 13.7%,包含7个主效位点(PVE>10%),与WSC含量相关的1个,与ADF和K含量相关的各3个;通过基因预测和功能注释筛选出13个与高丹草品质性状相关的候选基因,这为今后深入开展高丹草分子育种及功能基因研究提供了理论依据和新思路。

    Abstract:

    Quality trait is an important index to measure the forage quality, which plays an important role in the directional improvement and variety breeding of forage. High-quality forage grass can effectively improve the feeding efficiency of livestock and promote the sustainable development of animal husbandry. In this study, using 150 F2 individuals of Sorghum sudangrass hybrrid and their parents as materials. the QTL analysis was performed on five nutritional quality traits WSC, NDF, ADF, P and K by interval mapping methods in four different environments based on the previous constructed high density genetic linkage map of Sorghum sudangrass hybrid. and the candidate genes related to these traits were also screened within the confidence interval. The results showed that a total of 198 QTLs were detected with the threshold of LOD ≥3, of which 16 QTLs were repeatedly detected in different environments, The 16 stable QTLs mainly distributed on LG3, 4, 6, 7 and 8, with a single contribution rate from 10% to 13.7%, of which seven were major QTLs ( PVE > 10% ), including one QTL related to WSC content, three related to ADF content and three for K content. In addition, 13 candidate genes related to quality traits of Sorghum sudangrass hybrid were screened by gene prediction and functional annotation. It will provided theoretical basis and new ideas for further molecular breeding and functional gene research of Sorghum sudangrass hybrid.

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  • 收稿日期:2024-10-29
  • 最后修改日期:2024-12-19
  • 录用日期:2024-12-27
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